Transgenic mice used in this study were wildtype human a-synuclein (LHS), wildtype mouse synuclein (M1m), A53T mutant human a-synuclein (M53), first A30P mutant human a-synuclein line (TS2) and second A30P mutant human a-synuclein line (M30).
Transgenic mice (Syntg) were crossed to CSPa heterozygous Ko mice to generate CSPa KO/a-synuclein double heterozygous (CSP+/-Syntg). These mice in turn were bred to CSP+/- mice to obtain littermate CSP+/+, CSP+/- and CSP-/- mice that either express or lack an a-synuclein transgene. Similar crosses were done with CSPa and a/b-synuclein KO mice.
Antibodies for immunofluorescence staining were Q698 and Lb509. Immunoblotting used antibodies to CSPa, a-synuclein, g-synuclein, VCP, SNAP-25, synaptobrevin/VAMP 2, syntaxin 1 and complexins.