Ribé EM, Pérez M, Puig B, Gich I, Lim F, Cuadrado M, Sesma T, Catena S, Sánchez B, Nieto M, Gómez-Ramos P, Morán MA, Cabodevilla F, Samaranch L, Ortiz L, Pérez A, Ferrer I, Avila J, Gómez-Isla T. Accelerated amyloid deposition, neurofibrillary degeneration and neuronal loss in double mutant APP/tau transgenic mice. Neurobiol Dis. 2005 Dec;20(3):814-22. PubMed Abstract

REAGENTS/MATERIAL:

Mice studied were crosses of Tg2576 and TauVLW to give double mutant APP/tau+/+ (hereafter termed APPsw-tauvlw), mutant APP+/− (APPsw), mutant tau−/+ (tauvlw), and non-transgenic−/− animals.

Mouse coronal brain sections were probed with primary antibody (4G8 and 6E10 mouse anti-Ab 1:500, Chemicon, Temecula, CA; AT8 mouse anti-human PHF tau 1:50, Innogenetics, Belgium) and the appropriate secondary antibody (anti-mouse IgG1 1:200, Southern Biotechnology, Birmingham, AL; Vector ABC Kit). Sections were processed by Nissl staining for neuronal counts, Thioflavin-T and modified Bielschowsky.