Leyssen M, Ayaz D, Hébert SS, Reeve S, De Strooper B, Hassan BA.
Amyloid precursor protein promotes post-developmental neurite arborization in the Drosophila brain.EMBO J.
2005 Aug 17;24(16):2944-55.
REAGENTS/MATERIAL: Immunohistochemistry:The APPL antibody (used in a dilution of 1:10 000) was obtained from K White, the PDF antibody (1:1000) from P Taghert, the Elav (1:500) antibody from the Developmental Studies Hybridoma Bank (University of Iowa). For Myc staining the polyclonal antibody (Upstate), for GFP staining rabbit anti-GFP (Molecular Probes) (1:1000), and for APP the 4G8 antibody (1:100) from Abcam or the B63.5 antibody (1:8000) were used. For actin labelling, brains were incubated with 1:1000 TRITC-Phalloidin (SIGMA) in PBT for 2 h at room temperature.