The paper by Ziv et al. brings together two often disparate fields of study: immunology and neuroscience. The group of Michal Schwartz is one of a relatively few in the world who draws on tools of both trades to study how the immune and nervous systems intersect to influence brain function.

The authors propose the interesting hypothesis that the hippocampal (and olfactory) neurogenesis required for optimal functioning of the adult brain is dependent on cues from peripheral immune cells. It had been shown previously that inflammatory activation of the peripheral immune system can diminish neurogenesis in the brain. This work suggests that the converse, that is, that neurogenesis depends in some way on immune support, may also hold true.

The authors' use of SCID and nude mice for these studies is quite innovative, and the experiments carefully control for differences in genetic background that are known to influence adult neurogenesis. The decrement in BrdU+ cells, and specifically BrdU/DCX and BrdU/NeuN cells, in the immune-deficient mice is consistent with their...  Read more

The paper by Ziv et al. brings together two often disparate fields of study: immunology and neuroscience. The group of Michal Schwartz is one of a relatively few in the world who draws on tools of both trades to study how the immune and nervous systems intersect to influence brain function.

The authors propose the interesting hypothesis that the hippocampal (and olfactory) neurogenesis required for optimal functioning of the adult brain is dependent on cues from peripheral immune cells. It had been shown previously that inflammatory activation of the peripheral immune system can diminish neurogenesis in the brain. This work suggests that the converse, that is, that neurogenesis depends in some way on immune support, may also hold true.

The authors' use of SCID and nude mice for these studies is quite innovative, and the experiments carefully control for differences in genetic background that are known to influence adult neurogenesis. The decrement in BrdU+ cells, and specifically BrdU/DCX and BrdU/NeuN cells, in the immune-deficient mice is consistent with their hypothesis.

However, it is the reconstitution experiments examining neurogenesis after replenishing the immune system with normal or T cell-depleted splenocytes that to me forms the crux of this study. Restoration of normal neurogenesis by the introduction of donor splenocytes is the definitive proof that the neuronal precursor cell population is intact and simply requires external activation from the added immune cells. The description of these experiments might have benefited from more detailed display of this data on which to evaluate the results. For instance, in Figure 2 showing the first of the reconstitution experiments, the number of BrdU/DCX+ cells per dentate gyrus after addition of normal splenocytes (panel d) appears comparable to the number of BrdU/DCX+ cells found in unreconstituted SCID mice at the same age (panel a). The intended comparison is to SCID mice reconstituted with T cell-depleted splenocytes, but data for the level of neurogenesis in untreated mice would have been a good control to include in the same panel.

The reconstitution experiments are especially important in evaluating the data shown in Figure 4, which presents data of the study of neurogenesis in nude mice. The very disrupted DCX staining in the nude mice of Figure 4c suggests that the gene defect in these mice may affect neurogenesis in ways independent of T cell function. After all, the mice are also nude, and have hair follicle deficits that may have nothing to do with alterations in the immune system. For these experiments, the authors do plot the data one would have liked to see for the SCID experiments; specifically, they show untreated nude vs. nude + splenocytes, demonstrating that there is significant recovery of newly dividing cells in the hippocampus. Here it is worth noting that PCNA staining does not equal neurogenesis (PCNA, like BrdU, does not distinguish between cell types), and the experiment could be stronger if it provided the same comparison (untreated nude vs. nude + splenocytes) for BrdU/DCX+ double labeled cells to show a specific effect on neuronal production.

One goal to tackle for follow-up work is to convincingly connect the function of peripheral T cells to the effector microglia in the brain, and to explain how microglia then act on progenitor cells to increase neurogenesis. The present work shows T cells in the ventricles, where they might be able to directly influence the turnover and/or differentiation of precursor cells in the subventricular zone (the source of neurogenesis for olfactory bulb interneurons). It would be fascinating to know how they signal to microglia in the parenchyma of the brain to activate precursor cells deep in the dentate gyrus. Identifying the signaling factors used to communicate between these distant areas will solve the spatial paradox that exists based on the data available so far.

This paper should serve to start the neuroscience community thinking more seriously about the interaction of body and mind. There may be a lot more to it than most researchers or clinicians now realize.

View all comments by Joanna Jankowsky

Excellent article, and studies done were well-controlled. This article provides further validation of the communication between the central nervous system and the immune system. In this article, the authors showed that T cells (of the immune system) that reside in the central nervous system (CNS) can influence both neurogenesis and cognitive functioning independently. Under normal conditions, the resident T cells and microglia in the CNS are barely detectable, but when neurogenesis was enhanced by housing rats in an enriched environment, both T cells and microglia were activated. When neurogenesis was examined in mutant mice deficient in T cells, the authors found that neurogenesis was decreased compared to the control mice. It is interesting that even when the mutant mice were housed in the enriched environment, this did not help in increasing neurogenesis, as is usually seen in normal animals. However, when the mutant mice were injected with "splenocytes" containing replenished T cells, increased neurogenesis was seen when compared to mice depleted for T cells. Furthermore, the...  Read more

Excellent article, and studies done were well-controlled. This article provides further validation of the communication between the central nervous system and the immune system. In this article, the authors showed that T cells (of the immune system) that reside in the central nervous system (CNS) can influence both neurogenesis and cognitive functioning independently. Under normal conditions, the resident T cells and microglia in the CNS are barely detectable, but when neurogenesis was enhanced by housing rats in an enriched environment, both T cells and microglia were activated. When neurogenesis was examined in mutant mice deficient in T cells, the authors found that neurogenesis was decreased compared to the control mice. It is interesting that even when the mutant mice were housed in the enriched environment, this did not help in increasing neurogenesis, as is usually seen in normal animals. However, when the mutant mice were injected with "splenocytes" containing replenished T cells, increased neurogenesis was seen when compared to mice depleted for T cells. Furthermore, the authors found that the influence of CNS T cells in neurogenesis is partly mediated by microglial cells, because when they gave the mice minocycline (a drug that inhibits microglial activity), a significant decrease in neurogenesis was seen compared to controls.

What is also interesting in this study is that mere activation of resident T cells in the CNS does not result in increased neurogenesis. The authors showed that the antigen (or protein) that induces T cell activation has to be specific (i.e., proteins involved in brain plasticity) for effective involvement in neurogenesis. This is demonstrated when they examined two different types of transgenic mice (one that expresses T cell receptors that recognize myelin basic protein—protein that is involved in axonal growth—and another that expresses T cell receptors that recognize ovalbumin—general protein); they found increased neurogenesis and enhance learning ability only in the transgenic mice that express T cell receptors that recognize myelin basic protein when compared to their control counterparts. The thoroughness of the experiments presented in this article provides good evidence that the immune system is involved in maintaining CNS integrity. As the authors suggest, the results of their experiment may partially explain the association between age-related decline in neurogenesis and decreased immune functioning related to aging.

Further corroboration is needed before the evidence can be convincingly accepted. With regard to its applicability in Alzheimer disease, the results of this study may be limited. It should, however, trigger thoughts on the role of the aging immune system in influencing brain function, as well as on the bidirectional communication between the CNS and the immune system.

View all comments by Teresita Briones