Arrasate M, Mitra S, Schweitzer ES, Segal MR, Finkbeiner S.
Inclusion body formation reduces levels of mutant huntingtin and the risk of neuronal death.Nature.
2004 Oct 14;431(7010):805-10.
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Used PC12 and HEK-293 cultured cells and primary cultures of rat straiatal neurons prepared from embryos.
Transfected striatal neurons were probed with anti-GFP (1:500 dilution; Chemicon), anti-Htt EM48 (1:50 dilution; Chemicon) and anti-chicken or anti-rabbit Cy3-labelled antibodies (1:300 dilution; Jackson Immunochemical).