Lee JH, Lau KF, Perkinton MS, Standen CL, Rogelj B, Falinska A, McLoughlin DM, Miller CC. The neuronal adaptor protein X11beta reduces amyloid beta-protein levels and amyloid plaque formation in the brains of transgenic mice. J Biol Chem. 2004 Nov 19;279(47):49099-104. PubMed Abstract, View on AlzSWAN

REAGENTS/MATERIAL:

X11b transgenic mice were developed using mouse prion protein promoter and a carboxyl-terminal Myc-tagged full-length mouse X11b cDNA. APPswe Tg2576 mice were used for crossing with X11b mice.

Mouse brains were immunostained for X11b by using a rabbit X11 polyclonal antibody or mouse monoclonal antibody 9B11 to the Myc tag on the transgene; Ab deposits were detected using antibody 1E8.

Ab species were assayed using commercial ELISA kits and following the manufacturers' instructions. Ab(1-40) was assayed using humanAb(1-40)-(N) ELISA (IBL), and Ab(1-42) was detected using Innotest b-amyloid(1-42) ELISA (Innogenetics).