Tau P301L tg (Goetz) mice under control of the neuron-specific mThy1.2 promoter were used in this study.
Antibody HT7 (Innogenetics, 1:600) was used to detect human tau; AT100 (Innogenetics, 1:300) was used to detect tau phosphorylated at Thr-212/Ser-214; CP13 (P. Davies, 1:500) for detection of tau phosphorylated at Ser-202/Thr-205; and a monoclonal antibody (Innogenex, 1:500) was used to detect glial fibrillar acidic protein (GFAP). GLO-specific antisera were from S. Ranganathan and S. Staros (Fox Chase Cancer Center, Philadelphia) (1:50) and P. J. Thornalley (University of Essex, Wivenhoe Park, Colchester, U.K.) (1:10). For peroxidase/diaminobenzidine stainings, secondary antibodies were obtained from Vector Laboratories. Western Blots employed the following antibodies: HT7 (1:4,000), anti-GLO antiserum (S. Ranganathan and S. Staros; 1:500), anti-actin monoclonal antibody (Abcam, 1:5,000), and anti-GAPDH monoclonal antibody (BioDesign, 1:6,000).