Del Villar K, Miller CA. Down-regulation of DENN/MADD, a TNF receptor binding protein, correlates with neuronal cell death in Alzheimer's disease brain and hippocampal neurons. Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4210-5. PubMed Abstract

REAGENTS/MATERIAL:

DENN polyclonal antibody has been described(Zhang, Y., Zhou, L. & Miller, C. A. (1998) Proc. Natl. Acad. Sci. USA 95, 2586–2591).

Following antibodies were used in this study: JNK3 and phospho-c-Jun (Upstate Biotechnology, 1:1,000 and 1:200, respectively);

TRADD, FADD, and RIP (BD Pharmingen; 1:250, 1:200, and 1:1,000, respectively);

TNFR1 (sc-8436), TRAF2 (sc-7346), and JNK1 (FL) (sc-571) (Santa Cruz Biotechnology; 1:100, 1:200, and 1:1,000, respectively);

phospho-JNK and Myc (Cell Signaling Technology, 1:500 and 1:1,000, respectively);

GFP (BD Clontech; 1:2,000);

MAP2, b-actin, and NF200 (Sigma; 1:2,000, 1:1,000, and 1:500, respectively);

monoclonal AT8 (Endogen, 1:1,000);

and monoclonal 22C11, which recognizes the Ab region of APP (Chemicon; 1:1,000).