Drosophila stocks and GAL4 lines were obtained from the Bloomington Drosophila stock center. The UAS-tau(R406W) and the Cha-GAL4 lines were kindly provided by Drs. Mel Feany and Paul Salvaterra, respectively. Transheterozygous flies from a cross between a UAS line and a GAL4 line were used.
IHC analysis used primary antibodies: 3E6 monoclonal against GFP (1:1,000), rabbit polyclonal against nonphosphorylated form of h-tau (1:2,500), 12E8 monoclonal against h-tau (1:4,000), AT100 monoclonal antibody against PHF tau (1:1000). For MARCM analysis the primary antibodies used were rabbit anti-GFP and 12E8.
Western Blots were probed with primary antibodies: T14 (1:1000), 12E8 (1:4000), AT270 (1:1000), AT8 (1:500), AT100 (1:500), CP13 (1:500), PHF1 (1:500), Tau1 (1:1000), and 22C10 (1:200).