Studied insulysin-deficient (IDE) mice.
Prepared rIDE4020 antibody by immunization with nickel agarose-purified recombinant rat insulysin with an N-terminal six-histidine tag, which was expressed by a baculovirus system in Sf9 insect cells. The hIDE7-24 antibody was elicited by immunization with a synthetic peptide that corresponds to amino acids 7-24 (relative to the second ATG) of human insulysin that was coupled to keyhole limpet hemocyanin by a C-terminal cysteine residue.
These antibodies were used for immunodetection.
Sandwich ELISA for Ab40 and Ab42 were performed using the BNT77/BA27 and BNT77/BC05 antibody systems. BNT77, BA27, and BC05 were gifts of Takeda (Osaka).
In Western blotting the O443 antibody against the C-terminal 20 residues of APP was used for immunodetection of APP, CTF, and CTF.