Western Blot analysis was performed using goat anti-human jagged 1 antibody from Santa Cruz at 1:500 dilution.
For immunohistochemical staining on postmortem tissues the antibodies used were monoclonal anti-Notch 1, 1:200 (Labvision), polyclonal rabbit anti-Hes5, 1:2000 (Chemicon), monoclonal anti-GFAP 1:100 (Dako), polyclonal rabbit anti-S100beta, 1:50 (Dako), monoclonal 04, 1:50 (Chemicon), polyclonal rabbit anti-NG2 1:50 (Chemicon), monoclonal anti-PDGFRalpha,1:25 (R&D Systems) and monoclonal anti-TGFb1, 1:50 (Chemicon). Development was with biotinylated secondary antibodies and standard ABC protocol (Vector Labs) using either DAB or nitroblue tetrazolium for color staining.
Immunocytochemistry on astrocyte cultures used the same anti-jagged1 antibody as above at a 1:500 dilution. For Oligodendrocyte-3T3 cell cocultures monoclonal anti-MBP, 1:100 (Chemicon) or polyclonal anti-CNPase, 1:500 (gift of F.A.McMorris) were used, followed with biotinylated secondary antibodies and developed with ABC method and DAB.