Tomizawa K, Omori A, Ohtake A, Sato K, Takahashi M. Tau-tubulin kinase phosphorylates tau at Ser-208 and Ser-210, sites found in paired helical filament-tau. FEBS Lett. 2001 Mar 16;492(3):221-7. PubMed Abstract

REAGENTS/MATERIAL:
TPKI and TPK II were pruified from bovine brain as described in (Ishiguro et al, Takahashi et al.) Human recombinant tau-441 purchased from Panvera. Tau peptide K2 (191-224), partial phosphopeptides of tau pS202, pT205, pS208 (CPGDPGTPGSPRSRTPSL), pS210 (CPGDPGTPGSRSPRTPSL) and pS208/210 (CPGDPGTPGSPRSPRTPSL) were chemically synthesized employing solid-phase method and Biosearch Model 9500 or ABI Model 431A peptide synthesizer. The antibodies pS199 and pT205 as described in (Ishiguro et al.) The antibodies against pS208, pS210, pS208/210, and TTK-C were diluted to 15 ug/ml.

FUTURE DIRECTION:
in vivo study on the precisely timed appearance of TTK in the regulation of the hyperphosphorylation of tau by TPKI/GSK-3b is needed. The physiological role of TTK needs further elucidation.