Transgenic mice AD11 were used in this study.
Brain sections from AD11 mice and control mice were stained for neurofibrillary pathology using Gallyas silver impregnation method; the amyloid deposits were stained using Congo red, thioflavin S and the Campbell-Switzer silver method.
Amyloid deposites were immunohistochemically stained with monoclonal Abeta(17-24)4G8 (Signet), Abeta(1-40) from Sigma and Abeta(1-42) from Biosource.
Sandwich ELISA used Abeta 4G8 as the capture antibody and Abeta(1-40) and Abeta (1-42) for detection. For total Abeta the anti-R3660 against the NH-terminus of Abeta was used.
Antibody 4G8 was also used in Western blot.