Drug: Tauroursodeoxycholic acid(TUDCA)(Calbiochem) vs vehicle injected into wild-type mouse or transgenic mouse (R/6/2) model of Huntington's Disease (HD)(Jackson Labs).
Histology: Brain measurements. Apoptotic cells assayed with ApopTag in-situ detection kit (Intergen, Purchase, NY). Anatomic evaluation and volumetric analysis was performed on 30µm sections and stained according to the Nissl protocol using polyclonal antibody to anti-ubiquitin (1:1000)(Dako) and ABC kit (Vector Laboratories). Quantitiation of brain volume by Cavalieri method.