Wyss-Coray T, Lin C, Yan F, Yu GQ, Rohde M, McConlogue L, Masliah E, Mucke L. TGF-beta1 promotes microglial amyloid-beta clearance and reduces plaque burden in transgenic mice. Nat Med. 2001 May;7(5):612-8. PubMed Abstract

REAGENTS/MATERIAL:
Mice expressing TGF-b1 in astrocytes from GFAP promoter has been described in Wyss-Coray et al. Mice express hAPP from the PDGF b-chain promoter have been described in Games D. et al. Mice and AD patient brain sections were stained by standard immunoperoxidase techniques with ABC kits (Vector Labs) and 3,3'-diaminobenzidine plus hydrogen peroxide or by immunofluorescence-labelling procedures with FITC- and Cy5-tagged secondary antibodies (Vector Labs) Primary antibody 3D6 against human Ab1-3 (Elan Pharmaceuticals) FCA3340 [1:1000] against human Ab40, FCA3542 [1:1000] against human Ab42 (provided by F. Checler) F4/80, [1:60] against microglia F4/80 antigen (Serotec) Glut-1, [1:1000], anti-glucose-transporter-1 (Chemicon) AT8 against phosphorylated tau (Biosource) Antibody 266, anti-human Ab13-28, (Elan Pharmaceuticals) Mouse BV-2 microglia cell culture (gift from E. Blasi) exposed to Ab1-42 peptide (Bachem) [32P]-labeled antisense riboprobes identifying specific mRNAs (protected nucleotides, GenBank accession number): murine b-actin (nt 480-559, #M18194) porcine TGF-b1 (nt 999-1412, #M23703) murine TGF-b1 (nt 500-752, #M13177) human APP (nt 2468-2657, #X06989 of human APP fused with a notI linker to nt 2532-2636, #M24914 of SV40)

FUTURE DIRECTION:
Additional investigation will be necessary to characterize all cellular and molecular processes involved in the redistribution and overall clearance of amyloid induced by TGF-b1.