REAGENTS/MATERIAL: BAN50/BA27 and BAN50/BC05 antibody systems that detect Ab40 and Ab42, respectively. (Takeda)
BNT77 antibody was raised against amino acids 11-29 and recognizes amino terminally modified or truncated peptides of Ab
ECE-1a and ECE-1b were cloned by RT-PCR in 2 fragments jointed by a unique Pvu II site
ECE-1a forward 5' CAGGAATTCGCCACCATGCCTCTCCCAGGGCCTGGGCCTGC 3'
ECE-1b forward 5' CAGGAATTCGCCACCATGCGGGGCGTGTGGCCGCCC 3'
ECE-1 Pvu II reverse 5' CAGCAGCTTCCCCAGCTGGACC 3'
ECE-1 Pvu II forward 5' GGTCCAGCTGGGGAAGCTGCTG 3'
ECE-1 reverse 5' GCTCTAGATTACCAGACTTCGCACTTGTGAGGCGGG 3'
Antibody 22C11 antibody that detects secreted APP (Boehringer Manheim)
FUTURE DIRECTION: Analysis of ECE activity in AD and control individuals is necessary to determine the extent of the involvement of this enzyme family in the development of AD.