For western blots:
the Klc (63-90 and Klc1), Kif5b, APP (22C11), APP (4G8), tubulin, dynein intermediate chain (Dic), Kif3a, synaptotagmin, synaptophysin and Gap43 antibodies were used.
Additional antibodies used for western blots:
APP C-terminal (Chemicon), synapsin-I (Stressgen), Cox1 (Molecular Probes), A[beta]40 (Chemicon) or A[beta]42 (Chemicon), TrkA (Research Diagnostics; this antibody may cross-react with TrkB and TrkC), neurofilament-H (Smi-31, Sternberger), Bace goat polyclonal (Bace1, Chemicon), Bace1 rabbit polyclonal (Oncogene), Bip (Grp78) (Stressgen) and Pdi (Affinity Bioreagents).
The N-terminal presenilin-1 (NT) antibody, and the Aplp1 (Ct11) and Aplp2 (D2II) antibodies were provided by S. Sisodia. Presenilin-1 polyclonal antibody (Chemicon) gave the same results as the NT antibody from S. Sisodia; all blots shown are for NT.
For immunofluorescence staining:
APP C-terminal (Chemicon), Bace goat polyclonal (Chemicon), presenilin-1 (NT), and Cox1 (Molecular Probes) antibodies were used. In general, samples were analysed by Tris-glycine SDS-PAGE and western blots; for the A[beta] blots, Tris-tricine gels were used.
Sciatic nerves, DRGs or corpus callosum from three pairs of age-matched nonlittermate wild-type and APP-null mutant mice 17 were dissected. For double-ligation experiments, sciatic nerves of wild-type mice were ligated at the most accessible proximal and distal ends simultaneously