Polyclonal rabbit anti-huntingtin (P7), and anti-sheep antibody S830 against huntingtin were gifts J.C. Dorsman. Anti-huntingtin mouse monoclonal MAB2166 was from Chemicon. Anti-caveolin 1 was from Transduction Laboratories and anti-tubulin and anti-vimentin mouse monoclonal antibodies were from Sigma. Mouse and rabbit antibodies against a nine-residue fragment derived from influenza HA were purchased from BabCo.
Western Blot immunoprecipitations were performed with anti-huntingtin mouse monoclonal antibody (Chemicon) or anti-myosin light chain mouse monoclonal antibody (Sigma). Proteins were transferred onto nitrocellulose membranes and probed with anti-arfaptin 2 rabbit polyclonal antisera or anti-ubiquitin mouse monoclonal antibody.
Immunohistochemistry on brain sections were labelled with anti-arfaptin 2 rabbit polyclonal antisera and affinity-purified anti-huntingtin sheep polyclonal antibody.