Requirement of an intact microtubule cytoskeleton for aggregation and inclusion body formation by a mutant huntingtin fragment. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Muchowski PJ, Ning K, D'Souza-Schorey C, Fields S. Requirement of an intact microtubule cytoskeleton for aggregation and inclusion body formation by a mutant huntingtin fragment. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):727-32. PubMed Abstract

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REAGENTS/MATERIAL:

Double-label immunofluorescence was performed with monoclonal mouse anti-c-myc (clone 9E10, Santa Cruz Biotechnology), rabbit anti-Tub2 antisera (gift of Frank Solomon), goat anti-rabbit IgG-FITC conjugate (Santa Cruz Biotechnology), and goat anti-mouse IgG-AlexaFluor594 conjugate (Molecular Probes) as primary and secondary Abs.