Adeno-associated virus effectively mediates conditional gene modification in the brain. - AlzForum Alzheimer Research Paper of the Week


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Home: Papers of the Week

Annotation

	Kaspar BK, Vissel B, Bengoechea T, Crone S, Randolph-Moore L, Muller R, Brandon EP, Schaffer D, Verma IM, Lee KF, Heinemann SF, Gage FH. Adeno-associated virus effectively mediates conditional gene modification in the brain. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2320-5. PubMed Abstract

	Comments on Paper and Primary News

	Primary News: Adenovirus-Mediated Local Modification of Brain Genotype Comment by: Karen Duff, ARF Advisor
	Submitted 28 February 2002 \| Permalink	Posted 28 February 2002
	I think this is a good paper, and it adds to our transgenic toolbelt. It may, for example, allow us to turn pathology and off, to see if lesions such as plaques can be resolved once the source (e.g., elevated Aβ from an AβPP or PS1 transgene) is removed. Inducible transgenics have great advantages over constitutive ones, although there are also problems associated with the use of viral vectors. To be useful, though, it is important that new transgenic models in general be made with 'floxed' transgenes so that cre-directed control of transgene expression can be applied without having to make new mice. View all comments by Karen Duff

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REAGENTS/MATERIAL:

Multiple immunofluorescent labeling used antibodies against beta-galactosidase (b-gal; rabbit, 1:1,000 Cortex or mouse, 1:1,000 Promega), the neuron-specific nuclear protein NeuN (mouse, 1:50, R. Mullen, Univ. Utah), tyrosine hydroxylase (rabbit, 1:1,000, Chemicon), anti-GFAP (glial fibrillary acidic protein, guinea pig, 1:1,000, Advanced Immunochemical), choline acetyltransferase (ChAT; goat, 1:250, Chemicon), anti-CD4 and CD8 (mouse, 1:1,000, PharMingen), and anti-GFP (rabbit, 1:500, CLONTECH) to enhance detection of the reporter gene.