Reduced hippocampal neurogenesis in adult transgenic mice with chronic astrocytic production of interleukin-6. - AlzForum Alzheimer Research Paper of the Week


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Annotation

	Vallières L, Campbell IL, Gage FH, Sawchenko PE. Reduced hippocampal neurogenesis in adult transgenic mice with chronic astrocytic production of interleukin-6. J Neurosci. 2002 Jan 15;22(2):486-92. PubMed Abstract

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REAGENTS/MATERIAL:

Hemizygous offspring of the transgenic mouse line G167 which expresses IL-6 under control of the glial fibrillary acidic protein (GFAP) promoter were used for this study.

Mouse brain sections were incubated in primary antibodies: monoclonal rat anti-BrdU (Harlan Sera-Lab); polyclonal rabbit anti-GFAP (Chemicon); polyclonal guinea pig anti-GFAP (Advanced ImmunoChemical); monoclonal mouse anti-neuron-specific nuclear protein (NeuN, Chemicon); polyclonal rabbit anti-S100 (Swant); and polyclonal rabbit anti-ionized calcium-binding adapter molecule 1 (Iba-1 provided by Dr. Y. Imai).

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