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REAGENTS/MATERIAL:

The following primary antibodies were used: polyclonal anti-PS1 N-terminal fragment (NTF) (95.23), monoclonal anti-v-ATPase antibody (Osw2), monoclonal anti-lysobisphosphatidic acid (LBPA) (6C4), monoclonal anti-COP-I antibody CM1A10, monoclonal antibody WO2 (The Genetics Company) directed to residues 4-10 of human Amyloid, and monoclonal anti-C-terminal residues of Amyloid-beta 40 (G2-10)(The Genetics Company) and Amyloid-beta 42 (G2-11) (The Genetics Company). Polyclonal antibody PS1 CTF (29414) was provided by C. Elle. Monoclonal anti-calnexin and monoclonal anti-BiP/GRP78 antibodies were from Transduction Laboratories, monoclonal anti-PS1-loop antibody was from Chemicon, and polyclonal PS2 antibody was from New England Biolabs.