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REAGENTS/MATERIAL:
Antibodies used include:
rabbit anti-AMPKα phosphorylated at Thr-172 (Abcam Inc., Cambridge, MA, USA) and
rabbit monoclonal anti-AMPKα phosphorylated at Thr-172 (40H9) (Cell Signaling Technology, Danvers, MA, USA).
Mouse monoclonal antibodies against tau and Phosphorylated tau:
anti-total tau (CP27),
anti-tau phosphorylated at Ser-396/404 (PHF1),
anti-tau phosphorylated at Ser-202 (CP13),
anti-tau phosphorylated at Thr-231 (2E12),
anti-tau phosphorylated at Ser-235 (MC6),
anti-tau phosphorylated at Ser-409 (PG5) and
anti-tau phosphorylated at Ser-214 (CP3).
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