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Bosco DA, Morfini G, Karabacak NM, Song Y, Gros-Louis F, Pasinelli P, Goolsby H, Fontaine BA, Lemay N, McKenna-Yasek D, Frosch MP, Agar JN, Julien JP, Brady ST, Brown RH.
Wild-type and mutant SOD1 share an aberrant conformation and a common pathogenic pathway in ALS. Nat Neurosci.
2010 Nov;13(11):1396-403.
PubMed Abstract
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REAGENTS/MATERIAL:
Antibodies and reagents: In our experiments, we used antibodies to SOD1
(sheep PC077, the Binding Site;
sheep 574597, Calbiochem;
mouse SDG6 clone, Sigma Aldrich),
mutant SOD1 (C4F6 and A9G3,
Urushitani et al., 2007),
monoclonal mouse anti-KHC (H2)
(Morfini et al., 2009),
monoclonal rabbit anti-phospho-p38 MAPK (Cell Signaling #9215),
mouse anti-phospho-ERK (Santa Cruz) and
goat anti-phospho-GSK3 (Santa Cruz).
Secondary antibodies included horseradish peroxidase (HRP)-conjugated rabbit antibody to sheep IgG (Upstate, 12-342) and HRP-conjugated rabbit antibody to mouse IgG (Sigma, A9044).
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Paper Alert: SOD1 Deformed in Sporadic ALS, Suggests Common Pathway
