Boland B, Smith DA, Mooney D, Jung SS, Walsh DM, Platt FM. Macroautophagy is not directly involved in the metabolism of amyloid precursor protein. J Biol Chem. 2010 Nov 26;285(48):37415-26. PubMed Abstract

REAGENTS/MATERIAL:
Primary antibodies were supplied by the following sources: mouse anti-APP/APP-CTF (C1/6.1) (a generous gift from Dr. Paul Mathews, Nathan Kline Institute, NY, U.S.A.), mouse anti-MAP1 LC3 (2G6) (nanoTools, Teningen, Germany), rabbit anti-MAP1 LC3 (Cell Signaling, Danvers, MA, U.S.A.), rabbit anti-EEA1 (Cell Signaling), rabbit anti-CI-MPR (Abcam, Cambridge, U.K.), mouse anti-LAMP-1 (anti-mouse, Abcam), mouse anti-P70S6 kinase phospho-Thr389 (Cell Signaling), rabbit anti-total P70S6 kinase (Cell Signaling) and rabbit anti-NPC1 (Novus Biologicals, Littleton, CO, U.S.A.).

Murine Aβ Enzyme-linked immunosorbent assay (ELISA) Assay: Capture antibodies (C-terminal specific antibodies for Aβ40 and Aβ42, JRF/cAβ40/10 and JRF/cAb42/26, (Centocor, PA, U.S.A.) were used and a HRP-conjugated detection antibody JRF/rAb/2 (Centocor) that recognizes amino acids 1-17 of Aβ was used.