Paula-Lima AC, Adasme T, Sanmartín C, Sebollela A, Hetz C, Carrasco MA, Ferreira ST, Hidalgo C. Amyloid β-peptide oligomers stimulate RyR-mediated Ca2+ release inducing mitochondrial fragmentation in hippocampal neurons and prevent RyR-mediated dendritic spine remodeling produced by BDNF. Antioxid Redox Signal. 2011 Apr 1;14(7):1209-23. PubMed Abstract

REAGENTS/MATERIAL:
Primary antibodies used in this study: Rabbit anti-RyR3 (Chemicon, Temecura, CA), and mouse anti-MAP2 (Chemicon, Temecura, CA), mouse anti-RyR2 (Pierce Biotecnology, Rockford, IL), mouse anti-mHSP70 (Pierce Biotecnology, Rockford, IL), monoclonal anti-β−actin from Sigma (St Louis, MO), anti-glial fibrillary acidic protein (GFAP) from DAKO (Carpinteria, CA), rabbit anti-Drp-1 (Thermo Scientific, Rockford, IL), mouse anti-cytochrome C (BD Biosciences, San Jose, CA), and rabbit anti-COX IV (Cell Signaling, Danvers, MA).