Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS. Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain. J Neurochem. 2010 Oct;115(1):47-57. PubMed Abstract

REAGENTS/MATERIAL:
Antibodies used in this study include:
Rabbit anti-NEP (Chemicon, Temecula, CA, USA), anti-IDE (Abcam, Cambridge, MA, USA), goat anti-ECE-1 (R&D Systems, Minneapolis, MN, USA), synaptophysin monoclonal antibody, a generous gift from Dr William Honer, University of British Columbia, Vancouver, Canada, anti-β-Actin (Amersham, Piscataway, NJ, USA) and and anti-α-actin (Sigma-Aldrich, St Louis, MO, USA).
Sodium dodecyl sulfate–page acrylamide gel electrophoresis, western blot and protein quantification: mouse anti-Ab (Clone Ab9, a gift from Dr T. Golde, Mayo Clinic, Jacksonville, FL, USA), rabbit anti-NEP antibody (Chemicon, Temecula, CA, USA), mouse anti-IDE (Abcam, Cambridge, USA), goat anti-ECE-1 (R&D Systems), mouse anti-synaptophysin (EP10), mouse monoclonal antibody BAN50 (recognizing the amino terminus of Ab), mouse anti-b-actin (Amersham, Piscataway, NJ, USA) or rabbit anti-a-actin antibodies (Sigma-Aldrich).
Insulin-degrading enzyme-specific activity: ELISA plates were coated with capture IDE antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA).