Gijselinck I, Engelborghs S, Maes G, Cuijt I, Peeters K, Mattheijssens M, Joris G, Cras P, Martin JJ, De Deyn PP, Kumar-Singh S, Van Broeckhoven C, Cruts M. Identification of 2 Loci at chromosomes 9 and 14 in a multiplex family with frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Arch Neurol. 2010 May;67(5):606-16. PubMed Abstract

REAGENTS/MATERIAL:
After 3 months’ fixation in 10% buffered formalin, the brain was embedded in paraffin. Sections of 5μm were prepared from 3 frontocortical regions and Brodmann area 9, the olfactory bulb and tracts, cingulate gyrus, corpus callosum, superior temporal gyrus, hippocampus, parahippocampal gyrus, lateral occipitotemporal gyrus, occipital cortex with area striata, cerebellum, midbrain, and pons with locus coeruleus.
Histochemistry: The sections were examined using routine histopathological methods and immunostained using antibody AT8 against hyperphosphorylated tau (Innogenetics, Zwijnaarde, Belgium), anti-amyloid β (4G8) against residues 17 through 24 of (Signet, Dedham, Massachusetts), anti-prion protein (3F4) (Signet), anti-glial fibrillary acidic protein (Dako, Glostrup, Denmark), anti-CD68 (Dako), anti-ubiquitin (Dako), anti-p62 (Progen Biotechnik), rabbit anti-TDP-43 (Proteintech Group Inc, Chicago, Illinois), and anti-phospho-TDP-43 serines 409/410 [and see Labs].