Researchers led by Christian Haass at the University of Munich, Germany, discovered the stepwise cleavage with some twenty-first-century logic that Occam might have appreciated. Endoproteolysis of presenilin occurs in a cytoplasmic loop that stretches between transmembrane domains 6 and 7 of the protein. Processing splits the protease into two large, membrane-bound polypeptides. First author Akio Fukumori and colleagues realized that if they added a second protease site downstream of the usual break point, they could generate small polypeptides that could be easily analyzed by mass spectrometry (MS), uncovering the exact site, or sites, where normal presenilin cleavage takes place. Using amino acid substitution, they created a tobacco etch virus (TEV) protease site at the C-terminal end of the cytoplasmic loop, and after letting γ-secretase mature in HEK293 cells, they added TEV. The list of polypeptides they detected by MS was revealing.

The researchers found that endoproteolysis generates a major C-terminal protein fragment beginning with alanine 299, which is in agreement with previous findings. But they also found several longer, albeit less common, C-terminal fragments that start with amino acids as far upstream as methionine 292. Their presence suggests that presenilin endoproteolysis does not proceed by a simple exact cut, but results from successive cleavages, as in the case of APP processing. Several cleavage sites exist in APP (see Zhao et al., 2004) and Yasuo Ihara and colleagues at Doshisha University, Kizugawa, Japan, showed that γ-secretase cuts APP in a stepwise fashion, successively removing three-to-four amino acid peptides (see Takami et al., 2009).

If presenilin processing does proceed in an autocatalytic fashion, then one might expect that familial AD PS mutations, which alter the proteolytic processing of APP, would also alter PS endoproteolysis. In fact, this is exactly what the researchers found. Alanine 299 fragments still dominated the mix of peptides generated from FAD PS mutants, but the abundance of fragments starting with valine 296 and valine 293 grew when the most aggressive PS mutations were introduced. The analysis indicates that PS, like APP, is cleaved in successive steps, each removing three amino acids, strengthening the case that presenilin cleavage is autocatalytic. The researchers even found an explanation for one of the best arguments against autocatalysis. Mutations at glycine 384 in PS1 abolish γ-secretase activity but have no effect on PS1 cleavage. That alone would suggest a different protease is responsible for PS1 processing. But Fukumori and colleagues found that mutations at glycine 384 dramatically alter PS1 cleavage. Instead of the protein being fully processed to leave alanine 299 in the N-terminus of transmembrane loop 7, processing stops at either amino acid 292 or 296, rendering it a poor γ-secretase. The authors conclude that PS cleavage is autocatalytic, and furthermore, that stepwise endoproteolysis may be a common mechanism for processing intramembrane proteins.—Tom Fagan.

Reference:
Fukumori A, Fluhrer R, Steiner H, Haass C. Three-amino acid spacing of presenilin endoproteolysis suggests a general stepwise cleavage of gamma-secretase-mediated intramembrane proteolysis. J. Neuroscience 2010 June 9; 30:7853-7862. Abstract