Last Updated 1 March 2007
Q. I use the antibody goat anti-mouse RAGE on formalin-fixed, paraffin-embedded sections of mouse brain. The primary antibody comes from R&Dsystems AF 1179 goat anti-mouse RAGE. The secondary antibody comes from Dako Cytomation, Code E 0466, polyclonal rabbit anti-goat, biotinylated. I expect to see an imunhistological staining in the cytoplasma of neurons and in the epithels of blood vessels. I have worked with a lot of pretreatments, but always with the result of wrong pos. localisation in the nucleus instead of the cytoplasm of the neurons. Can anyone help me? Thanks, Susanne—Posted 1 March 2007
Q. I am trying to find the following presentation: Aisen, P. Mehran, M et al.: Clinical data on Alzheimed after 12 months of treatment in patients with mild to moderate Alzheimer's disease. Presented at the 9th International Conference on Alzheimer's Disease and Related Disorders. July 18, 2004, Philadelphia PA. I am willing to pay to have a copy. Thank you for your help and cooperation. Penny Philpot—Posted 25 February 2007
Q. Hi, I am searching for an antibody against Br-cadherin (also called cadherin 12 or neuronal cadherin-2). This antibody should be suitable for immunohistochemistry and react on human tissue.
Thanks, Mihovil—Posted 25 February 2007
Q. Hi! I am trying to block Cdk5 expression in primary neuronal
cultures by RNA intereference, using Dharmacon smart pools and
oligofectamine but without great success. Does anybody have any suggestions/protocols???
Thank you, Xenia Antoniou—Posted 10 February 2007
Q. Hi, I have difficulties with the DsRed Polyclonal Antibody
(#632397) from BD Biosciences on cryosections: I get a very unspecific
staining, but my positive control is fine. For further information: I have
only 1-2 copies of the dsRed transcript (knock-in). What are your
experiences with that antibody? Would/can you recommend any other dsRed
antibody that is working on cryo sections?
Thanks, Kristina—Posted 18 January 2007
Q. I am searching for an antibody against presenilin-1. This antibody
must be human specific and must not react with mouse material
Thanks, Savioz—Posted 18 January 2007
Q. Hi, I'm looking for a monoclonal antibody against the terminus
of either alpha-secretase product, i.e. 1-16 or 17-42. The antibody must
not cross-react with full-length beta amyloid 1-42 or APP. If you have
ever heard of such a thing, please let me know
Thanks, John Schloendorn,
Arizona State University—Posted 18 January 2007
Q. I am desperately looking for an m266 antibody to apply in primary
neuronal cultures. Does anyone know if it is commercially available or how
could I get it otherwise?
Thanks a lot, Inna Slutsky, PhD
Dept. of Physiology and Pharmacology
Sackler School of Medicine, Tel Aviv University
Ramat Aviv, Tel Aviv 69978, Israel—Posted 11 January 2007
Q. I am desperately seeking a DYRK1a antibody that can IP and
immunodeplete DYRK1a from rat brain high speed centrifuged supernatant.
Ideally an antibody that does NOT cross react with anything else in rat.
What concentration/conditions would you use to for best immunodepletion
results? Reference: Kentrup et al., "Dyrk, a Dual Specificity Protein
Kinase", No.7, Vol.271, J.Biol. Chem, 1996.
King's College, London—Posted 14 December 2006
Q. I am in need of endothelial nitric oxide synthase polyclonal
antibody raised in rabbit or mouse against bovine.
Thanks, Pramod—Posted 14 December 2006
Q. I am desperately seeking a specific antibody for the protein
sAPPalpha (secretory product of APP protein and a product of alpha secretase enzyme).
With reference to literature "Rene etal., 2004, Therapeutic effects of
PKC activators in Alzheimer's disease transgenic mice, PNAS 101(30)", I was
aware of the monoclonal antibody 6E10 to detect sAPPalpha.
Please let me know at the earliest, it would help to purchase the antibody for my experiments.
Thanks, Maria Juliet—Posted 8 December 2006
Q. Dear AD reseachers, to validate our recent studies of FAD mutations in presenilins and ER Ca
signaling (Tu, H. et al. (2006) Cell 126, 981-993) we would like to
perform experiments with fibroblasts from human PS1 and PS2 FAD patients.
Would you be interested to collaborate with us on these studies and provide
us with samples of human PS1 and PS2 FAD fibroblasts?
The only cells we could get from Coriell are PS1-A246E, but we would like to test more FAD mutants.
Thank you, Ilya Bezprozvanny—Posted 28 October 2006
Q. I'd like to buy the antibody against PS2 N-terminal from
Alexis (monoclonal antibody to presenili 2 (APS 21) ) for western blotting.
Does anybody know if it is a good antibody?
Thanks in advance, Anna Paterlini—Posted 28 October 2006
Q. Does anyone know of antibodies that specifically recognize
Abeta n-38? How about anti-P3? (must be selective in each
case for the species noted)
Thanks, Bill Netzer—Posted 28 October 2006
Q. Hi. I'm looking for a good antibody against mouse presenilin 2
N-terminal for western blot!
Thanks in advance, Anna Paterlini—Posted 28 October 2006
Q. Hi! I'm desperately looking for the cell line named 7PA2 (CHO
cells) and I would be very grateful if you could either provide me with a
vial or if you'd know where to purchse them from.
Cheers, Barbara Bennani-Baiti—Posted 16 October 2006
Q. Does anyone know of a commerical source for monoclonal
antibodies against IAPP (amylin)?
Thanks, Louise Scrocchi—Posted 3 October 2006
Q. I'm looking for purified neuron-axon filament protein (NAFP).
Thanks, Anand Swamy—Posted 26 September 2006
Q. Hi all, I'm looking for the 3D structure of monoclonal antibody
raised against abeta(1-42). If possible, Please send me the 3D strcuture of
this abeta immunoglobulin structure in .pdb format.
Thanks and regards, Raj—Posted 21 September 2006
Q. Please, could you send me information about coss-reactivity for
measurements of chromogranin-A, RIA in pig serum or plasma.
Yours sincerely, Jelena Raukar—Posted 10 September 2006
Q. I am looking for a few brain tissue samples for alzheimer's to
aid my research. Is there anybody out there who can help me procure some? I
think I have found something quite relevant but I need some more
samples to confirm it.
Please help!!!!!! Tessi Sherrin—Posted 29 August 2006
Q. I am currently seeking antibodies that will recognise mouse VEGF
isoforms (188,164 and 120) on a western blot. Any info will be
greatly appreciated. I have tried a couple of different antibodies
to date and can only see 164.
Thanks. Sheila Harris—Posted 29 August 2006
Q. We are desperately seeking somebody who works with recombinant
alpha-1-antichymotrypsin as we have projects running but no more
rACT. Please, if you can spare some ACT contact me.
Thanks in advance. Henrietta Nielson
Lund University, Wallenberg Laboratory 2nd floor
Dept of Clinical Sciences Malmö, Malmö University Hospital entrance 46
205 02 MALMÖ, SWEDEN
Phone: +46-40-331414 or +46-704-953991; Fax: +46-40-337041—Posted 3 August 2006
Q. Hi, I am looking for an antibody against rat brain Cyp46 (CHOLESTEROL 24-HYDROXYLASE) for Westerns and ICH.
Any suggestions are welcome!!!
Thanks. Selma Kanazir, PI
Inst. for Biological Research, Despota Stefana Blvd. 142
11060 Belgrade, SCG
Tel: +11 2078 344, Fax: +11 2761 433—Posted 12 June 2006
Q. Hi, I am looking for an antibody to detect the C terminal tail of presenilin
(using as an inmunogen the few terminal amino acids).
I've tried with P7854 from Sigma, but it didn't work well. Does anybody know where can I find a good antibody?
Thanks. Violeta Lamarca Gay—Posted 11 April 2006
Q. I am looking for an antibody to selenoprotein S. It is a surface protein of the ER,
and is involved in the processing and removal of misfolded proteins.
Thanks. Kathleen L. Rea Fureigh, Ph.D.
Department of Geriatrics,
University of Arkansas for Medical Sciences
Donald W. Reynolds Institute on Aging
Tel: (501) 526-5810, (501) 526-5830 (fax)—Posted 27 March 2006
Q. Hi. I'm looking for an antibody (for western blot and immunohistochemistry) aganist
human 17β-hydroxysteroid dehydrogenase type 1and 2 . I would appreciate it if any one could let me know if there is any source for these antibodies.
Thanks to you all. Salama
UTMB, Galveston, TX—Posted 16 March 2006
Q. Hi. I am seeking antibodies to ptpn21 and Nrg3, both Human.
Thanks. Janice Lam
University of Hong Kong—Posted 16 March 2006
Q. Hi. I am looking for a monoclonal antibody specific against the so-called A-beta globulomer
(a 12-mer molecule generated by incubation with –of all things- SDS!!).[Ref: J Neurochem, 2005, 95, 834-847].
Does anyone work with/on this type of oligomeric form of A-beta? If anyone does have this oligomeric form,
can you please tell me how to generate it from the monomeric form? I have tried it but can’t get a clean band on the gel.
Thank you. Rajshenkhar Kore
Dept. of Physiology & Biophysics, Slot #505, Univ. of Arkansas for Medical Sciences,
4301 W. Markham Street, Little Rock, AR 72205—Posted 4 March 2006
Q. Hi. I'm looking for an ELISA kit for rat's CSF melatonin.
Thank you. Liguanghua—Posted 4 March 2006
Q. Hi. I am looking for an antibody against mouse or human Huntingtin gene for this region of the mouse genome:
Thank you very much, Mohammed Idris, PhD Student
Stazione Zoologica 'Anton Dohrn'
Villa comunale 1, Naples, Italy 80121
Phone 00-39-081-5833228 Mobile 00-39-3472686839—Posted 2 March 2006
Q. Hi. Does anyone know a commercial source of polyclonal antibodies to melatonin. I need these antibodies
for immunohistochemical studies in the mouse.
Thank you very much. Oscar Rojas-Espinosa PhD
Mexico—Posted 24 February 2006
Q. Hi all. I am looking for anti chicken ovalbumin antibody which I can use for Facs analysis.
If someone knows anything about it the help is very welcome.
Thanks in advance. Satwinder
VU University Medical Center, Netherlands—Posted 16 January 2006
Q. Our lab is in need of monoclonal DsRed antibody.
We have tried to buy some from clontech, but it has been on backorder for the past two months.
If anyone could send us a small aliquot, we could replace it when we get our order.
Thank you. Natasha Naumova
Fox Chase Cancer Center—Posted 11 January 2006
Q. I'm working on a project with protein AIPL1, but I don't have the
antibody. I would appreciate if anyone can give me some information
were can I get it.
Thanks. Josif Mirceski—Posted 11 January 2006
Q. I am looking for an antibody that recognizes residues 23-29 of beta amyloid
Thanks. Antonella Prisco
Istituto di Genetica e Biofisica "A. Buzzati Traverso", CNR
via Pietro Castellino 111, 80131 Napoli, Italy—Posted 28 December 2005
Q. Hi, I am looking for a reliable antibody for the detection of HMG-CoA reductase in WB. Thanks for the help.
Jakub Golab, M.D., Ph.D.
Department of Immunology, Center of Biostructure Research
The Medical University of Warsaw
tel.: (*4822) 599-2198 fax: (*4822) 599-2194—Posted 28 December 2005
Q. Hi, I would like to try some anti-phospho-tau immunostaining on parafin-embedded mouse tissues.
Would anyone have specific ordering information for Innogenetics?
Their AT8 antibody seems to be the most popular. Thanks for any help. Steve Crocker—Posted 9 December 2005
Q. Hi, I would be highly thankful if somebody could provide us with Amyloid beta specific oligomer
antibody for detecting/quantitating invitro oligomers formed in presence of our test molecule. Thank you. Veer Bala Gupta
CFTRI, Mysore, India—Posted 9 December 2005
Q. Hi, I am about to complete a set of experiments for which I need
anti-phosphoserine 880 GluR2 antibody.
This antibody is not commercially available anymore. Has anybody got any?
Thank you very much. Elsa Martinez—Posted 2 December 2005
Q. Hi! I am begining to work within this subject and would like to
know how to handle gamma secretase inhibitors such as L685458,
commercialized by SIGMA. Is it reliable to resuspend it for example in PBS
and freeze it at minus 20? for how long? is it stable?
Thanks very much for any information. Karen Amelia Nahmod—Posted 22 November 2005
Q. I would appreciate it if someone could give us antibodies for detecting phosphorylation dependent
and independent forms of Tau and of senile plaques. This is basically to do our pilot studies in a mouse model that is
deficient in phosphatase but shows neurofibrillary tangles and senile plaques. We wish to check whether the observed
NFTs are phosphorylated Tau or not.
Thanks! Subramaniam Ganesh
Biological Sciences and Bioengineering
Indian Institute of Technology, Kanpur 208016, INDIA—Posted 19 November 2005
Q. We want to see if a new compound can target on RAGE. We need the plasmid full-length RAGE (RAGE-FL) and the RAGE
cytoplasmic deletion mutant (RAGE-Δ). Can someone help us, and send these plasmids and their antibodies?
Thank you! Hanxin—Posted 8 November 2005
Q. I need reliable antibodies to two postsynaptic proteins: PSD-95 and GABA-A alpha subunit. And if someone has
a good idea for another postsynaptic protein I would like to interchange protocols.
Thanks! Jorge Parodi—Posted 3 November 2005
Q. Has anyone ever seen a reduction of amyloid plaques in mice after a SHORT (1 month or less) immunization? (Could you provide a reference, please? )
Thanks. Antonella Prisco
Istituto di Genetica e Biofisica "A. Buzzati Traverso", CNR
via Pietro Castellino 111, 80131 Napoli, Italy—Posted 3 November 2005
Q. I'm doing a masters thesis about rheumathoid artritis! I'm going to do
ELISA to investigate ferritin! Could you recommend to me some reliable antibodies to L- and
H-chain ferritin, please?
Thanks! Barbara Muz—Posted 19 October 2005
Q. I'm looking for the famous 12E8 antibody against tau phosphorylated
at S262, in KXGS motif. Does anyone know if it is commercially available
or how could I get it otherwise?
Thanks a lot! Carlos Fitzsimons, PhD
LACDR/Medical Pharmacology Dpt., Leiden University
Einsteinweg 55, P.O. Box 9502, 2300 RA Leiden, The Netherlands—Posted 30 September 2005
Q. I would like to know if there are commercially available human and mouse chitinase-1 chintinase-2 antibodies?
Thanks! Lifeng—Posted 30 September 2005
Q. I'm looking for an antibody that will on Western Blot pick up
neurofilament M in mouse tissue that is specific for the phosphorylated form of the protein.
If you are aware of some place I could purchase this antibody, please reply.
Thanks! Anita Gnezda
Department of Pathology, IUSOM, indianapolis IN—Posted 30 September 2005
Q. I'm looking for anti human presenilin 1 antibody with epitope located between 105-280 AA residues
( between first transmembrane domain and putative site for 'presenilinase' activity ).
Thanks in advance! Lukasz Bojarski
International Institute of Molecular and Cell Biology, Warsaw, Poland—Posted 19 September 2005
Q. I’m interested in working with the IGFBP3 antibody from Gene Tex
(product id = 16328),
but I need some more information about how/ if it works in Western blotting and eventually immunoprecipitation.
Has anybody already used it and could ensure me that it really works?
Thanks a lot! Heide Vogel
Institute for Neuropathology, Munster Germany—Posted 31 August 2005
Q. I am using OBTOO30-Monoclonal Rat -anti-BrdU (as primary antibody) on
mouse brain. The pregnant mums or animals were injected with Brdu in
vivo and then their embryos were processed for paraffin. I am not
getting any results and I think this is because of the primary antibody
(OBT0030). Is it the right antibody for this kind of experiment?
Thanks! Noura—Posted 23 August 2005
Q. Does anyone know of a good rabbit antibody to ApoE in mouse tissue that
can be used for immunohistochemistry?
Thanks! Emily Choi
UBC, Canada—Posted 23 August 2005
Q. I am looking for an alpha7 beta integrin that reacts to mouse
myoblasts. I will use it for flow cytometry and hopefully it is conjugated to a flourochrome. Could someone help me out?
Thanks! Leslie So
UBC, Canada—Posted 18 August 2005
Q. We are desperately seeking for Amyloid AA antibody that would work on
Western blots and immunohistochemistry in mouse tissues.
Research Scholar, Bioorganic and Neurochemistry Lab,
Central Leather Research Institute, Chennai-600 020. INDIA—Posted 18 August 2005
Q. Does anyone have experience with unfixed (frozen only) brain samples and alpha synuclein antibodies?
I have problems detecting the antigen in this tissue. Could someone help me out and get in contact with me?
Daniel Havas M.Sc.
JSW-Research Forschung GmbH,
Rankengasse 28, 8020 Graz, AUSTRIA—Posted 13 July 2005
Q. Dr. Sergiu Calmanovici advises that he has created a new antibody, 480 Tamra, which produces, in various animal models,
neuropathology that is indistinguishable from Alzheimer's pathology. Limited amounts are available.
Contact Sergiu for more information.—Posted 8 July 2005
Q. I am looking for a human kalikrein 6 (neurosin, protease M) antigen (native or recombinant protein) for calibration
of my ELISA method. Does anybody know where I can buy this antigen? Does anyone out there donate any such protein, preferably
commercial, or generated (purified) by individual researchers? Could someone help me out? Any info would be greatly appreciated.
Tomasz Wielkoszynski MD, PhD
Medical University of Silesia, Zabrze, Poland—Posted 1 July 2005
Q. I need an anti-ovalbumin antibody for research use. I
wonder which companies have this kind of product?
New York University—Posted 15 June 2005
Q. I am looking for information regarding the availability of F(ab')2 fragments of 3D6, and techniques on how to
label antibody with dye. I really appreciate any response.
Thanks. Wellington Pham
Massachusetts General Hospital, Charlestown, MA—Posted 31 May 2005
Q. I am doing my PhD at the Hacettepe University, Faculty of Medicine,
Department of Medical Biology, Ankara, Turkey. I am searching for an
antibody that is specific for human ST6Gal1. I learned that it is not
commercalized yet. I will be grateful if anyone could help me!
Thanks. Burcu BALCI, MSc
Hacettepe University Faculty of Medicine
Department of Medical Biology, 6th floor, 06100
Sihhiye, Ankara, TURKEY
Tel: +90 312 305 2541 Fax:+90 312 309 6060—Posted 18 April 2005
Q. I am looking for an antibody that recognizes Ser 159 phosphorylated Cdk5.
I know that Santa Cruz biotechnology has one already, but I need to try one other from a different supplier.
Does anyone know if any other company supplies the same...?
Best regards. Senthil Saravanamuthu
NIH, Bethesda—Posted 28 March 2005
Q. I need an ab for immunocytochemistry for Dax1/NROB1 in mouse, rat and
human tissues. Can anyone recommend any commercial one?
Thank you! Markku Pelto-Huikko
Professor, Department of Developmental Biology
FIN-33014 Tampere University
Phone: +358-(0)3-2156644 Fax: +358-(0)3-2156170—Posted 25 Ferbruary 2005
Q. We are desperately seeking for anti-ApoE Ab that would work on Western blots with RAT brain proteins,
We had no success with Calbiochem Abs.
Thanks, Selma Kanazir
Laboratory for Molecular Neurobiology
Institute for Biological Research
Belgrade,Serbia and Montenegro—Posted 18 Ferbruary 2005
Q. Has anyone had any success in using Chemicon's MAB348 (formely Roche
22C11) to cause neuronal toxicity in the last year or so? We have tried
three different lots of this antibody and have not been able to see the
same effect on neuronal death that we and others have published when
using the 22C11 from Roche.
Thanks in advance, Donna McPhie
Belmont MA , 02478—Posted 21 January 2005
Q. I need an antibody directed against poly-alanine repeats. Does anyone know
if one exists and if so where I can get it from? Any info would be greatly appreciated.
Thanks, Sabrina Mosaheb—Posted 6 January 2005
Q. We are looking for an anti-sAPPbeta. Would you please give us some help?
Thanks a lot! Lin Zou
Shanghai Institute of Biological Sciences,
Chinese Academy of Sciences,
Shanghai 200031, P.R.C.—Posted 20 December 2004
Q. We are looking for an anti-OVA (chicken egg ovalbuimn) antibody that can be used in Immunofluorescence (IF) applications.
We can pay the shipping !
Thanks in advance. CEPPI Maurizio, PhD
Lab for Dendritic Cells biology - P.Pierre group, Centre d'Immunologie de Marseille-Luminy
Parc Scientifique de Luminy case 906, 13288 Marseille cedex 09, FRANCE
Tel: +33 4 9126 9158, Fax: +33 4 9126 9430—Posted 3 December 2004
Q. I am a scientist from EnVivo Pharmaceuticals. I am asking if people know any immunostaining protocols for anti-Abeta42
antibodies without using formic acid treatment.
Thanks a lot, Hsin-Pei Shih, PhD
480 Arsenal Street, Building 1, Watertown, MA 02472
Phone: 617-225-4272 Fax: 617-2254267—Posted 3 December 2004
Q. I wish to contact other researchers who have, and would be willing to send me, an APP gene . I wish to make Tg mouse expressing this gene.
Thank you, Chunyi Yung
Department of Pharmacology, Sun Yat-sen University of Medical Sciences
74 zhongshan road, Guangzhou,Guangdong
P.R.China, 510080—Posted 3 December 2004
Q. If anyone has a staining protocol for KMn04 congo Red for AA and AL amyolid I sure would like to have a copy of it.
Thanks, Patricia Lewis—Posted 20 November 2004
Q. I am looking for anti-rat C5a and serum amyloid A. An ELISA kit would be even better.
Thank you, Denis Gris
Robarts Research Institute,
Box 5015, 100 Perth Drive
London, Ontario N6A 5K8—Posted 10 November 2004
Q. I am looking for paravulbumin antibody; is a Ca2+ binding protein, is excellent new neuroanatomical markers which
can be utilized to selectively visualize certain neurons and pathways in CNS.
Best! Vandana Dass
Dept. of Anesthesia, University of Arkansas for Medical Science
Little Rock, AR 72205.   Phone: (501) 526-7150—Posted 2 November 2004
Q. I am desperately seeking phosphorylation-independent neurofilament H
antibody that stains NFs well in formaldehyde fixed cultured neurons
(using immunofluorescence) with no cross reactivity with M or L. I tried some commercially available antibodies from Sigma and Chemicon
but none of them worked for my purpose.
Thanks! Yanping Yan
Center for Molecular Neurobiology, The Ohio state University
056 Rightmire Hall, 1060 Carmack Road, Columbus, OH 43210
Phone: (614) 292-1357 Fax: (614) 292-5379—Posted 20 October 2004
Q. I wish to contact other researchers who have, and would be willing to send me, a Thy-1 promoter construct.
I wish to make Tg mouse using this promoter.
Thanks. Dr. Gaku Sakaguchi
Discovery Research Laboratories,
SHIONOGI & CO., LTD.
1405, Gotanda, Koka, Shiga 520-3423, Japan
Phone: +81-748-88-6529 Fax: +81-748-88-2783—Posted 19 October 2004
Q. We are desperately seeking HMG-CoA reductase antibodies. We will pay shipping!
Thanks! Dr. Gunter Eckert
ZAFES-coordinator & project manager, Interdisciplinary Pharmacology
University of Frankfurt, Biocentre N260 R 1.09, Marie-Curie-Str. 9
D-60439 Frankfurt / Germany
Phone: +49-69-798-293-78; Fax: +49-69-798-293-74—Posted 6 October 2004
Q. I am looking for IgG from rhesus monkey serum, should be 95% essentially
salt free (SDS-PAGE or HPLC). Please let me know if you have some available for immediate use.
Thanks! Sunita Sharma—Posted 16 September 2004
Q. I am desperately seeking antibody raised against SEVKM (666-670) residues of
APP corresponding to C-terminus of sAPP-beta.
Thank you, Neelima Chauhan
Assistant Professor: Department of Anesthesiology,
Department of Anatomy and Cell Biology, University of Illinois at Chicago
Health Scientist: R&D, Jesse Brown VA Medical Center Chicago
Phone: 312-569-7747 (Direct); 312-569-6166/7426 (Department)
—Posted 7 September 2004
Q. I wish to contact other researchers who have, and would be willing to send me, an APP promoter
construct. I wish to carry out a luciferase reporter assay using this promoter.
Thank you, Emma Jones—Posted 31 August 2004
Q. I need a TrkA negative cell line.
From the literature I found SK-N-MC, IMR-32, CHP134, IMR5. I ordered
SK-N-MC but it was not negative for both transcript and protein. Is there
any other well known cell line tested/used/known to be used as a TrkA
Thank you for your time, Korcan Ayata—Posted 30 August 2004
A. The following paper might be helpful to you:
Green SH, Rydel RE, Connolly JL, Greene LA. (1986) "PC12 cell mutants that possess low- but not high-affinity nerve growth factor receptors
neither respond to nor internalize nerve growth factor." J Cell Biol. 102(3):830-43.
Four mutant PC12 pheochromocytoma cell lines that are nerve growth factor (NGF)-nonresponsive (PC12nnr) have been
selected from chemically mutagenized cultures by a double selection procedure: failure both to grow neurites in the presence of NGF
and to survive in NGF-supplemented serum-free medium. The PC12nnr cells were deficient in all additional NGF responses
surveyed: abatement of cell proliferation, changes in glycoprotein composition, induction of ornithine decarboxylase, rapid changes
in protein phosphorylation, and cell surface ruffling. However, PC12nnr cells closely resembled non-NGF-treated PC12 cells in
most properties tested: cell size and shape; division rate; protein, phosphoprotein, and glycoprotein composition; and cell surface morphology.
All four PC12nnr lines differed from PC12 cells in three ways in addition to failure of NGF response: PC12nnr cells failed to internalize bound
NGF by the normal, saturable, high-affinity mechanism present in PC12 cells. The PC12nnr cells bound NGF but entirely, or nearly entirely,
at low-affinity sites only, whereas PC12 cells possess both high- and low-affinity NGF binding sites. The responses to dibutyryl cyclic AMP
that were tested appeared to be enhanced or altered in the PC12nnr cells compared to PC12 cells. Internalization of, and responses to,
epidermal growth factor were normal in the PC12nnr cells ruling out a generalized defect in hormonal binding, uptake, or response mechanisms.
These findings are consistent with a causal association between the presence of high-affinity NGF receptors and of NGF responsiveness and
internalization. A possible relationship is also suggested between regulation of cAMP responses and regulation of NGF responses or NGF receptor affinity.
Dr. Elliot Mufson,
Rush-Pre?byterian-St. Luke’s Medical Center, Chicago—Posted 3 September 2004
Q. I am searching for a 10D5 antibody to amyloid
for testing rabbit brain beta-amyloid. Please tell me if you know of one.
Thanks! Chantelle Sephton—Posted 24 August 2004
Q. I am seeking antibodies for detection of PS1, GAP43 and/or Kif5b, mainly
by immunocytochemistry and if possible also by western blotting.
Hopefully is there anybody who has experience with these antibodies I need and eventually
spare some to test them in my cells. Any information would also help a lot.
Thanks a lot. Anca Laura Tirniceriu
University of Munster, Institute for Neuropathology
Domagkstr. 19, 48149 Munster Germany
Phone: 0049 251 83-52362 Fax: 0049 251 83-56971—Posted 22 August 2004
Q. I am a biochemistry PhD candidate and I want to produce a mAb against melatonin.
I would apreciate if you kindly let me know about production, application and availabilty of this product in the market.
Thanks. M. Sokhtanloo—Posted 15 August 2004
Q. I am despartely seeking an APC conjugated gamma delta TCR antibody for
staining murine thymocytes, does anyone know of a source?
Much thanks in advance. --Susan C. McKarns, Ph.D.
Laboratory of Cellular and Molecular Immunology
BG 4 / Room 111 - MSC 0420
National Institute of Allergy and Infectious Diseases, NIH
Phone: 301-451-2058 or (lab) 301-451-3812 Fax: 301-496-0877—Posted 11 August 2004
Q. Does anyone know of a corporation that sells diagnostic Kit for Luteinizing Hormone (Colloidal
Gold) for a moderate price?
Any replies would be appreciated. Li Zhiguo—Posted 11 August 2004
Q. I'm desperately seeking antibodies to detect selectively Ab 1-40 and Ab 1-42,
by western blotting and immunocytochemistry. The antibodies we had used at the lab are A-8326 (by SIGMA)
wich has a weak detection by western blotting and the other one is an anti-human amyloid b 1-42
by Pharmingen (BD Biosciences) which is supposed to be suitable for immunocytochemistry but we've had poor results with it
and it definitely doesn't work in western blot. I really need advice in this matter and, even better, I hope that some one
could spare some good antibodies.
In advance, thanks a lot. Mary Carmen Vázquez Rodríguez
PhD Student, Center for Cell Regualtion and Pathology "Joaquín Luco V."
Faculty of Biological Sciences, Pontifical Catholic University of Chile, Alameda #340
Phone: 56-2-686 2722 Fax: 56-2-686 2717—Posted 22 July 2004
Q. I am writing to ask for your help. I am studying for a Master's degree in neuroscience in Wuhan University in China.
At present, I am engaged in investigating the Hyperphosphorylated tau leading to Alzheimer disease with hippocampus of Sprague Dawley(SD) rat.
During the experiment, however, I encountered a great difficulty for lack of Tau monoclonal antibodies including PHF-1, Tau-1, 12E8, AT-8
with which we can illustrate and locate the site of Hyperphosphorylated tau by immunohistochemistry. The specific functions of these antibodies
are as follows: PHF-1 is an antiphospho Ser396/404 tau mAb,12E8 is an antiphospho Ser262/356 tau mAb,AT-8 is an antiphospho Ser202/Thr205 tau mAb.
But only Tau-1 recognized the protein when Ser199/202 are dephosphorylated. Could you possibly provide these antibodies for me?
Also,I would be very grateful if you could tell me how to get access to these antibodies, so that I could achieve perfect experiment results with your help.
I look forward to hearing from you.
Thank you very much indeed. YangBo—Posted 19 July 2004
Q. I am looking for an antibody to beta-Amyloid 1-40/42(10D5)
for testing beta-Amyloid in rabbit brain.
Thanks, Huf—Posted 8 June 2004
Q. I am looking for an antibody that recognizes human RIL protein.
Anti-human TRIP6 antibody will also be extremely helpful.
Thank you in advance. Olga Guryanova—Posted 8 June 2004
Q. For my ELISA I am searching an
HRP-labeled antibody that can recognize the part of APP before the beta
cleavage site (so recognise sAPPbeta). Does anybody know where I can buy such an antibody?
Thanks, Elke Van Rossen
Galapagos, Gen. De Wittelaan L11 A3, 2800 Mechelen
Phone: +32/15.342.925—Posted 27 May 2004
Q. I am looking for any information on polyclonal, preferably rabbit, antibodies to polyglutamine.
Has anyone had one of these or any experience in trying to generate one?
Thanks, Alex Osmand—Posted 18 May 2004
Q. I am looking for an antibody to the intravesicular/extracellular domain (amino-terminal
epitope) of mouse/human APP that does not cross-react with APLP1 or APLP2. It should work
great in immunocytochemistry and immunohistochemistry. Ideally, it should be a mouse
Thank you for your help. Virgil Muresan—Posted 25 April 2004
Q. If anyone out there knows where I can purchase m266 for a good price, I
would really appreciate it.
Thank you. Brian Barr—Posted 6 March 2004
Q. I am looking for an ELISA for secreted sAPPalpha / full length APP.
Thank you. Andreas Ebneth—Posted 6 March 2004
Q. I am looking for a monoclonal antibody that recognises mouse
Thank you. Sebastien Hebert
Center for Human Genetics, O.& N.,
Herestraat 49, B-3000 Leuven, Belgium
Tel:+32 16 345878, Fax:+32 16 347181—Posted 24 February 2004
Q. I am seeking rat apo-A1 and an antibody against rat apo-A1. The antibody may be raised in mouse,
rabbit, horse, dog, pig, etc.
Thank you. Yaoming Wei—Posted 20 February 2004
Q. I am looking for an antibody to LBPA (lysobisphosphatidic acid). I prefer
a monoclonal antibody that recognises the LBPA.
Thanks a lot. XingJie Liang
National Cancer Institute, Lab of Cell Biology, Tel:301-435-6305—Posted 20 February 2004
Q. I'm looking for an antibody (preferably monoclonal) that recognises the human FE65 factor.
Thank you. Stephen McIlroy
Queens University of Belfast, Department of Geriatric Medicine
Whitla Medical Building, 97 Lisburn Road
Belfast, BT9 7BL UK
Phone +44(0)2890 272034, Fax +44(0)2890 325839—Posted 16 February 2004
Q. We are looking for Ab's suitable for western blots that target enzymes in the lipogenic,
lipolytic, gluconeogenic and ketogenic pathways.
If anyone could help with possible sources we would appreciate it.
Thank you. Adam Kennedy
Obesity Research Station
Joslin Diabetes Center, Harvard Medical School
1 Joslin Place, Boston MA—Posted 12 February 2004
Q. I am looking for an ELISA to measure ADAM 10 protein levels and an assay
for ADAM 10 activity in human brain extracts
Thank you. Ken Moya
CEA-CNRS URA 2210
Service Hospitalier Frederic Joliot
4, Place du General Leclerc, 91406 Orsay France—Posted 29 January 2004
Q. Hello, I need to get some information regarding
immunohistochemistry of 11beta Hydroxysteroid Dehydrogenase Ezzyme. What
antibodies and technique could be used?
Thank you. Aida Al-Wahaibi—Posted 29 January 2004
Q. Can anyone provide me some information on how to
find the NF-M purified protein (human) since I want to use it as my western blotting positive control!
Thank you. Si Wu—Posted 29 January 2004
Q. We have just purchased some Merck GFAP, NFKB antibodies but there is no data available for the use of the
antibody for immuohistochemistry. Could anyone let me know if they have used it for this purpose and if so could they provide me
with protocol regarding the fixatives and concentrations for LM and or TEM using fluorescence and immunoperoxidase
Thank you. E.Philip Jesudason, Senior Research Fellow
C/O Dr. R. Jayakumar,
Bioorganic and Neurohemistry Laboratory
Central Leather Research Institute,
Adyar, Chennai 600 020. Tamil Nadu, India—Posted 24 January 2004
Q. I am looking for an antibody specific for polyglutamine oligomer that does not recognize monomeric polyglutamine.
Could someone help me out?
Thanks in advance. Gomathi Kannayiram, Research Scholar
C/O Dr. R. Jayakumar,
Bioorganic and Neurohemistry Laboratory
Central Leather Research Institute,
Adyar, Chennai 600 020. Tamil Nadu, India—Posted 21 January 2004
Q. I am looking for an antibody directed against the c-myc epitope used as a tag, obtained from a
different species than mouse or rabbit.
Thanks for your help. Veronique Boyer, PhD
Tel: National: 04 76 76 88 81 ou 93 12 ou 5518
International: +33 4 76 76 88 81 or 93 12 or 5518
FAX: National: 04 76 76 58 22
International: +33 4 76 76 58 22—Posted 9 January 2004
Q. I am desperately seeking monoclonal antibodies raised against EFRH (3-6) residues of N-terminal A-beta peptide with
IgG2a and IgG2b isotypes.
Thank you. Neelima Chauhan, PhD
Research Health Scientist,
Research & Development (537/151), VA Chicago Health Care System
820 South Damen Avenue, Chicago, IL 60612
Phone: 312-569-7747 (Direct); 312-569-6166/7426 (Department)—Updated 5 January 2004, posted 15 December 2003
Q. I have been seeking an antibody to anti amyloid domain (for e.g KLVYA) in beta amyloid and amylin,
in order to look at its inhibition state. Does anyone out there donate any such antibody, preferably commercial,
or generated by individual researchers?
Thank you. Dr. Seema Jagota
C/O Dr. R. Jayakumar, Research Scholar,
BioOrganic and Neurochemistry laboratory,
Central Leather Research Institute, Adyar, Chennai 600 020.Tamil Nadu, INDIA—Posted 17 December 2003
Q. Does anyone know of a corporation that sells 4G8 crude for a moderate price?
Any replies would be appreciated. Brian Barr—Posted 8 December 2003
Q. We are looking for an anti mouse Abeta antibody for the pursuit of brain immunoblot and/or
ELISA experiments with NON-APP transgenic mice.
Thanks for your help. Danny Michaelson
Department of Neurobiology, Tel-Aviv University, Israel 69978—Posted 1 December 2003
Q. I am looking for an antibody specific for Abeta oligomer that does not recognize Abeta monomer. Could someone help me out?
Thanks a lot, Dr. Nobuyuki Suzuki
DAIICHI PHARMACEUTICAL CO..LTD. Tokyo R&D Center
16-13, Kita-Kasai 1-Chome, Edogawa-ku, Tokyo 134-8630 Japan
Tel: +81-3-3680-0151 Fax: +81-3-5696-8718—Posted 23 November 2003
Q. I am looking for an antibody recognizing MOUSE serum amyloid A, ideally raised in rabbit or goat.
Thank you, Elena Galea
Anesthesiology, University of Illinois at Chicago—Posted 16 November 2003
Q. I am looking for an ELISA kit for measuring APP in rat brain extracts.
Thanks, Ken Moya—Posted 5 November 2003
Q. I am looking for a company that makes an ELISA kit for measuring APLP1.
Thanks, Jun Guan—Posted 23 October 2003
Q. I am looking for an antibody specific for GFAP (rat) for ELISA but done on the untouched cells
after fixation and permeabilisation. Can anyone help with protocol and where to get antibodies.
Thanks a lot. Dr. Anna Price
ECVAM, Institute for Health and Consumer Protection
European Commission Joint Research Center
Via Fermi 1, 21020 - Ispra, Italy
Tel. +39 0332 789412 Fax: +39 0332 785336—Posted 20 October 2003
Q. I am looking for a monoclonal anti-mouse APP CTF antibody that can be used
on Western blot. Can anyone help me out?
Thanks! Jos Tournoy, MD
Center for Human Genetics, KUL, VIB
Herestraat 49, 3000 Leuven Belgium
Tel 32/16/346227 fax: 32/16/347181—Posted 17 October 2003
Q. I am looking for an antibody specific for the Amyloid intracellular domain
(AICD) without detecting any other C-terminal fragments (ie antibody against
aa 49-60 of APP, numbering from the first residue of Abeta). Could someone
help me out?
Cheers, Giuseppe Verdile
Department of Psychiatry and Neurogenetics, University of Western Australia,
Sir James McCusker Alzheimer's Disease Research Unit
Phone: +61 8 9346 6656/6388 Fax: +61 8 9346 6666—Posted 15 October 2003
Q. We are currently looking for recombinant sAPPbeta'. I.e. secreted APP that
is derived from full length APP processed by BACE at the Glu11 position
(sAPPbeta+ amino acids D A E F R H D S G Y). Can someone inform us where to
buy this recombinant protein.
Thanks a lot, Kurt Spittaels—Posted 25 September 2003
Q. I need an antibody that CAN distinguish the NFkB p105 form and not the p50.
Thanks! Honor Hugo—Posted 25 September 2003
Q.I am and have been desperately seeking an antibody to phosphorylated
fyn, in order to look at its activation state. Does anyone out there
know of any such antibody, preferably commercial, or generated by
Thanks! Dr. Gil Ho
University of California, San Diego, Dept. of Neurosciences, La Jolla, CA—Posted 3 September 2003
Q. I am looking for human or murine Myostatin cDNA. Please inform me
where I can buy them or get them.
Thanks! Nathalie Scamuffa
—Posted 27 August 2003
Q. I am looking for an antibody to melatonin receptor 1/2. Does anyone know
a commercial supplier or lab producer?
Thanks a lot! Fides Meier
Psychiatrische Universittsklinik, Neurobiologisches Labor, Wilhelm Klein-Str. 27, 4025 Basel—Posted 4 August 2003
Q. I need to find an antibody to cdx-2 that works very well in
immunohistochemistry on paraffin sections.
Thanks, Ivana Saroto—Posted 1 August 2003
Q. I am seeking a kit to detect Bovine Il-1beta, can anybody help please?
Thanks, Lisa Scott—Posted 24 July 2003
Q. Has anyone used 4G8 or 6E10 in flow cytometry? If so, can you recommend the best commercial supplier
of the antibodies for this purpose, and are they available fluorescently labeled?
Thanks, Rosalynde—Posted 10 July 2003
Q. I would like to use monoclonal antibodies specific for beta amyloid, clone Nos. 3D6 and 21F12.
I'd appreciate if anyone would inform me of the commmercial sources.
Thanks, Yuji Yamada—Posted 10 July 2003
Q. Can anyone recommend an antibody against the N-terminal of human APP that
can be used in an ELISA?
Thanks, Almar Kuipers—Posted 10 July 2003
Q. Has anyone ever tried 4G8 or any other ABeta in adult guinea pig brain by
immunohistochemistry? The only published record of any AD type pathology in
GP brain dates from 1910 (Perusini) - surely someone has looked in the last 97 years.
Thanks, Jim Finley—Posted 6 July 2003
Q. I am looking for an insulin antibody measuring kit, i.e. human insulin measured
in rat (after dosing with human insulin). Are there ELISA kits for quantitating insulun antibodies?
Thank you, Vinaya Chaitanya—Posted 12 June 2003
Q. For a trial on differentiating mouse embryonic stem cells I am looking
for an antibody against Neurofilamen 68 kDa (NF-L). If anybody could share some AB
with me I would greatly appreciate it.
Thanks, Kathrin Banach
Research Assistant Prof., Dept. of Physiology, Loyola Univ.
Phone (708) 216 9113 Fax (708) 216 6308—Posted 2 May 2003
Q. We have just purchased some Bachem Guinea-pig Galanin antibody but there is
no data available for the use of the antibody for immuohistochemistry.
Could anyone let me know if they have used it for this purpose and
if so could they provide me with information regarding the fixatives
and concentrations for LM and or TEM using fluorescence and
immunoperoxidase detection systems?
Thanks, Dr Susan E Luff,
Transmission electron microscopy and training consultant,
Monash Micro Imaging
PO Box 13C, Clayton Campus, Monash University,
Vic 3800 Australia
Phone 61 3 99052746 Fax 61 3 99052733—Posted 30 April 2003
Q. I am looking to buy an anti-alpha 7 integrin antibody that cross-reacts ?ith human tissue.
The mouse reactive version, clone 6a11, is commercially available now but does not react with human. Help!
Many Thanks, Andrea Sinanan
020-7915-1199 TELEPHONE AND VOICEMAIL
020-7915-1213 or 1199 FAX
07957-144210 MOBILE—Posted 25 April 2003
Q. I am looking for antibodies which can stain endogenous Presenilin-1
in mouse IHC. If you know the sources (either commercially available
or the labs), please drop me a line.
Thanks, Yonghua Pan—Posted 2 April 2003
Q. I am looking for a non-Rabbit mAb against p35.
Thanks, Othman Ghribi—Posted 20 March 2003
Q. I am a graduate student working for my Ph.D. I am in need of antibody for the
A beta region of Amyloid precursor Protein. I will be using the antibody for my western
blotting studies in mice tissues. Can anyone provide me a small aliquot.
I will acknowledge the kind help whenever necessary
Thanks, T. Mani—Posted 19 March 2003
Q. In my IP and western-blot experiments about APP, I find that
there are many bands recognised by APP N-ternimal and C-ternimal
antibodies. I can't comfirm what the types of these bands really are. Who
can tell me
(1) the molecular weights of APP ,including
APP695 (immature APP695, mature APP695 such as
N-gly-APP, N-O-Gly-APP......) APP751, APP770, APLP1, APLP2.
(2) Is APP659 more abundant than APP751 and APP770 in brain?
Thanks, Shen Chengyong—Posted 18 March 2003
Q. Does anyone know which company or lab could supply me with the 8E5 antibody for APP?
Thanks, Julia Kellersmann—Posted 13 March 2003
Q. We are planning to produce Fab fragments of an antibody against
the C-terminus of sAPPalpha (equivalent to N-terminus of Abeta).
As we could not find any report of Fab fragments of a suitable
antibody (only of 4G8, which does not serve) in the literature, we
are somewhat apprehensive, because there is the possibility of
papain destroying the antigen-binding site. Has anybody done this with 6E10?
10D5 would serve, too, but I am unable to discover who, if anybody,
sells 10D5 at the moment. Is it Elan or Athena Diagnostics, or does
Athena Neurosciences exist as independent company? Thanks, Matthias Gralle
Laboratorio de Biofisica Quimica de Proteinas
Bioquimica Medica, ICB, CCS
Cidade Universitaria, CCS, bl.H2, sl. 19
Rio de Janero, Brazil
Phone: (0055)(0xx21) 2562-6790,
Fax: (0055)(0xx21) 2270-6789—Posted 10 March 2003
Q. I am looking for an antibody capable of detecting by
immunocytochemistry epitopes in the mouse APP region corresponding to
the amino-terminus of the A beta peptide. Does anyone know of an
antibody similar to the 6E10 antibody (recognizes the first 17
amino-terminal amino-acids in human Abeta) that would work with mouse
APP? Thanks, Virgil Muresan
Department of Physiology & Biophysics.
School of Medicine, E553
Case Western Reserve University
Lab Phone: 216-368-6309,
Office Phone: 216-368-4766—Posted 6 March 2003
Q. I am research scholar looking for a laboratory that
can spare a small amount of abeta antibody (recognize both the abeta 1-40 and 1-42 in rat cell lines),
working in immunocytochemistry and Western blotting (astrocytes and glial cells). Thanks, Jayaraman Murali—Posted 1 March 2003
Q. I'd like to find out if?there's a commercial supplier of antibodies
to serum cleaved Tau (C-Tau) similar to the ones used by Zemlan's group.
I'm interested in using them for both ELISA & Westerns. Thanks, Alice Loo—Posted 28 February 2003
Q. Can anybody recommend to me a commercial or laboratory source for GFAP and its
Thanks, Louis F M Campos—Posted 24 February 2003
Q. Can anybody tell me which company or lab could supply me with the two
antibodies used in western blot and immunohistochemistry: Ab against human alpha-synuclein
and Ab against rat alpha-synuclein?
Thanks, Tong Liu—Posted 18 February 2003
Q. Does anyone have a comercial or laboratory source for glypican 3 antibody useful in cell sorting?
Thanks, Please contact Miki Blumenberg—Posted 10 February 2003
Q. I am looking for an antibody against galanin that is raised in an animal
other than a rabbit, for immunohistochemical studies. If anyone is aware
of a commerically or privately available antibody I would be greatful if
they could contact me. Dr Susan E Luff
Transmission electron microscopy and training consultant,
Monash Micro Imaging
PO Box 13C, Clayton Campus, Monash University,
Vic 3800 Australia
Phone 61 3 99052746 Fax 61 3 99052733—Posted 5 February 2003
Q. Do you know if there are commercially available kits for beta-catenin and GSK-3 beta?
Many thanks and kind regards, Pierre—Posted 3 February 2003
A. For antibodies I would suggest that you purchase the antibodies from commercial
sources. The most reliable antibodies to beta-catenin and GSK that we
have used are from Transduction laboratories (now owned by BD Biosciences
I believe) and Upstate biotech. Upstate also sells purified protein,
like GSK. Finally, we buy the phospho-specific catenin antibodies
from Cell Signaling (from New England Biolabs). Edward Koo—Posted 4 February 2003
Q. Is there a commercially available antibody that immunoprecipitates both Ab40/42?
Is the 1E8-4b or similar clone about? I need this ab to wrap up my PhD.
Thanks, David Anderson—Posted 3 February 2003
Q. Does anybody know which MW for APP I should expect by using MAB348 from
Chemicon (clone 22C11) and MAB343 (still from Chemicon) in Western blot? I use mouse
embryonic cells transiently overexpressing human APP751. And in your opinion which is
the best antibody to detect human APP and Abeta1-42 in the same kind of cells?
Thanks in advance, Fulvio Celsi—Posted 29 January 2003
Q. Is it possible to use FLIP as an inhibitor of the FAS pathway, does anyone
know whether there is commercially available FLIP which will enter cells and
inhibit. Alternatively, how can we inhibit specifically the FAS pathway? Thanks, Brendon Noble—Posted 20 January 2003