 |
 |
   |
|
| Alzforum Home Active Topics Email Notify Search Help Register Login |
| Antibodies | |
 Alzforum Bulletin Boards : Desperately Seeking : Antibodies |
| Topic: amyloid antibody |   |
| Author | Message |
|
Rufus Akinyemi FMCP
Posts: 1 |
   Topic: amyloid antibodyPosted: 30 Oct 2008 at 9:26am |
|
How specific is 4G8 for insoluble amyloid fibrils in amyloid plaques? I'm getting significant intracellular IR (suggesting intracellular amyloid presence) while using 4G8 to stain for extracellular amyloid.
|
|
 IP Logged |
|
|
jim ttttttt
Posts: 9 |
Posted: 06 Nov 2008 at 1:58pm |
|
have you seen this research group? check out their message board they are out of the university of washington ..someone there might be able to help you ..... http://boinc.bakerlab.org/rosetta/ check out this group also http://imminst.org/
|
|
|
IP Logged |
|
|
Antibodies Moderator
Posts: 12 |
Posted: 10 Nov 2008 at 8:10pm |
|
Professor Irina Alafuzoff of Kuopio University Institute of Clinical Medicine, Kuipo, Finland, has very graciously sent us the following information (professor Alafuzoff is a member of the Alzheimer Research Forum):
"We have dealt with this problem in a recent MS we sent to Laboratory Investigation. I hope it will be concidered for acceptance as this issue surely needs clarifications.
"Shortly, 4G8 is not specific as it cross reacts with APP and thus even in a child one will see a lot of intracellular labelling. Based on biochemical analyses all or at least most of the intracellular labelled protein is in soluble form. The only way to test this issue in a study is in addition to an amyloid-beta antibody includ antibodies to APP C and N terminals. Regarding the solubility one has to carry out biochemical analyses."
|
|
|
IP Logged |
|
| |
||
Bulletin Board Jump |
||
