Methods: DB, WB
Source: anti β-Amyloid oligomers
(paper, Mary Lambert et al.,
Northwestern University, Evanston, IL, USA)
De Felice, 2009
Reacts in: Human, Mouse
immunogern = Aβ oligomers (amyloid β-derived diffusible ligands, ADDLs), mixed with Freund's adjuvant (we suggest that the NU-1 and NU-4 epitope is three-dimensional, in which aggregation of Aβ1–28 to form a dimer is necessary to align correct amino acids in the binding pocket, perhaps several from each single peptide)
The species recognized by NU-4 in western blots included lower order and prominent higher order oligomers (12–24mers). Aβ1–40 monomer was not recognized in immunoblots by NU-4. NU-4 binds strongly to Alzheimer's disease brain samples but not to control brain samples
- De Felice FG, Vieira MN, Bomfim TR, Decker H, Velasco PT, Lambert MP, Viola KL, Zhao WQ, Ferreira ST, Klein WL. Protection of synapses against Alzheimer's-linked toxins: insulin signaling prevents the pathogenic binding of Abeta oligomers. Proc Natl Acad Sci U S A. 2009 Feb 10;106(6):1971-6. PubMed.
- Jimenez S, Baglietto-Vargas D, Caballero C, Moreno-Gonzalez I, Torres M, Sanchez-Varo R, Ruano D, Vizuete M, Gutierrez A, Vitorica J. Inflammatory response in the hippocampus of PS1M146L/APP751SL mouse model of Alzheimer's disease: age-dependent switch in the microglial phenotype from alternative to classic. J Neurosci. 2008 Nov 5;28(45):11650-61. PubMed.
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