Jak2 phos Tyr1007/1008 (C80C3)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Bovine, Human, Mouse
immunogen = synthetic phospho peptide (KLH-coupled) corresp. to a region surrounding Tyr1007/1008 of human Jak2
detects endogenous levels of Jak2 when phosphorylated at Tyr1007/1008. This antibody can also detect single phosphorylation at either 1007 or 1008. Cross-reactivity may be observed with phospho. Tyk2
-
Jak2 phos Tyr221
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Mouse
immunogen = synthetic phospho peptide (KLH-coupled) corresp. to a residues surrounding Tyr221 of human Jak2
detects endogenous levels of Jak2 when phospho. at tyrosine 221
-
Jak2 phos Tyr1007/1008
Cell Signaling
polyclonal
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Mouse, Rat
immunogen = synthetic phospho peptide (KLH-coupled) corresp. to a residues surrounding Tyr1007/1008 of human Jak2
detects endogenous levels of Jak2 only when phosphorylated at tyrosine 1007/1008; may cross-react with phospho-Jak1
-
Jak2 (D2E12)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Pro841 of Jak2
detects endogenous levels of total Jak2 protein; no cross was observed with other family members
-
p70 S6 Kinase phos Thr389
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr389 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389; also detects p85 S6 kinase when phosphor. at the analogous site (Thr412) and possibly S6KII phosphor. at Thr401
-
p70 S6 Kinase phos Thr421/424
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr421/Ser424 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr421/Ser424. This antibody also detects p85 S6 kinase when phosphor. at the corresponding sites (Thr444/Ser447)
-
CKIIα
Novus Biologicals
polyclonal
Rabbit
(antisera, affinity purified, Tris-saline, BSA, azide)
-
Human
immunogen = human CKIIα1; epitope maps to a region between residue 300 and 350
detects CKIIα
-
CKIIα prime
Novus Biologicals
polyclonal
Rabbit
(antisera, affinity purified, Tris-citrate, phosphate buffer, azide)
-
Human
immunogen = human CKIIα2; epitope maps to a region between residue 300 and the C-terminus (residue 350)
detects CKIIα
-
CKIδ
Novus Biologicals
polyclonal
Rabbit
(antisera, affinity purified, Tris-saline, BSA, azide)
-
Human
immunogen = human CKIIδ; epitope in an internal region (C-DREERLR HSRNPATR )
detects CKIIδ 48kDa in WB
-
JNK/SAPK phos Thr183/Tyr185 (G9)
Cell Signaling
monoclonal
IgG1
Mouse
(affinity purified, sodiumHEPES, BSA, glycerol, azide)
-
Human, Hamster, Mouse, Rat, S. cerevisiae
immunogen = synthetic phospho peptide (KLH-coupled) corresp. to residues surrounding Thr183/Tyr185 of human SAPK/JNK
detects endogenous levels of p46 and p54 SAPK/JNK dually phosphorylated at Thr183 and Tyr185; does not recognize endogenous levels of phosphorylated p44/42 MAPK or p38 MAP kinase
-
SAPK/JNK
Cell Signaling
polyclonal
Rabbit
(affinity purified, sodium HEPES, BSA, glycerol)
-
Bovine, Zebra fish, Human, Hamster, Mouse, Rat, S. cerevisiae
immunogen = GST/human JNK2 fusion protein
detects endogenous levels of total SAPK/JNK protein
-
Jak2 phos Tyr1007/1008
Calbiochem EMD Biosciences
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, BSA, azide)
-
Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresp. to aa surrounding the Tyr1007/1008 phosphorylation sites of human JAK2
recognizes the ~130 kDa JAK2 protein phosphorylated at Tyr1007/1008 in LIF-treated 3T3-L1 cells
-
Jak2 phos Y1007/Y1008
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, no preservative, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide surrounding to the epitope -Ey yK- with dual phosphorylation sites at Tyr1008 and Tyr1009 of JAK2 from human, mouse and rat origins.
JAK2 with dual phosphorylated sites of Tyr1007/1008; no crossreaction with non-phosphospecific peptide
-
Jak2 phos Tyr1007/1008 (E132)
Abcam
monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic phospho peptide corresp. to a region surrounding Tyr1007/1008 of human Jak2
only detects JAK2 phosphorylated on Tyrosine 1007 and 1008; detects a band of ~120 kDa (predicted molecular weight: 130 kDa)
-
Jak2 phos Y1007/Y1008
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, no preservative, affinity purified, conj. biotin)
-
Human, Mouse, Rat
immunogen = synthetic peptide surrounding to the epitope -EyyK- with dual phosphorylation sites at Tyr1008 and Tyr1009 of JAK2 from human, mouse and rat origins
JAK2 with dual phosphorylated sites of Tyr1007/1008; no crossreaction with non-phosphospecific peptide
-
Jak2 phos Y1007
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, , azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 1007 (K-E-Yp-Y-K)
detects endogenous levels of JAK2 only when phosphorylated at Tyrosine 1007
-
Jak2 phos Y221
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, , azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 221(Q-D-Yp-H-I)
detects endogenous levels of JAK2 only when phosphorylated at Tyrosine 221
-
Jak2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from adjacent to the C-terminus of JAK2 protein; seq. is identical in human, rat and mouse
JAK2; no cross reaction with JAK1 or JAK3
-
Jak2
Abcam
polyclonal
IgG
Rabbit
(liquid, TBS, no preservative, affinity purified, conj. biotin)
-
Human, Mouse, Rat
immunogen = synthetic peptide surrounding to the epitope -EyyK- without any phosphorylation of JAK2 protein from human, mouse and rat origins
JAK2 without any phosphorylated sites of Tyr1007/1008; no crossreaction with phosphospecific peptide
-
Jak2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
-
Human, Mouse
immunogen = synthetic peptide: F R D L A L R V D Q I R D N M A G (C-terminal region of human JAK2) conj. to KLH
JAK2
-
Jak2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse
immunogen = synthetic peptide (human) derived from JAK2 around the phosphorylation site of Tyrosine 221
detects endogenous levels of total JAK2 protein
-
Jak2
Abcam
polyclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 1007 (K-E-Yp-Y-K).
detects endogenous levels of total JAK2 protein and may cross react with phospho Jak1
-
Jak2 (691R5)
Abcam
monoclonal
IgG2b , κ
Mouse
(liquid, PBS, BSA, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = recombinant fragment from human JAK2 expressed in E.coli
JAK2
-
Jak2 (M126)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa190-315 mapping at the N-terminus of mouse JAK2
JAK2
-
Jak2 (HR-758)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope mapping within an internal region of mouse JAK2
JAK2
-
Jak2 (C-20)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of mouse JAK2
JAK2
-
Jak2 (C-14)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping within an internal region of mouse JAK2
JAK2
-
Jak2 (N-17)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the N-terminus of human JAK2
JAK2
-
Jak2 phos Tyr1007/1008
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the N-terminus of human JAK2
detection of dually phosphorylated Tyr 1007 and Tyr 1008 of JAK2
-
Jak2 phos Tyr1007/1008
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the N-terminus of human JAK2
detection of dually phosphorylated Tyr 1007 and Tyr 1008 of JAK2
-
Jak2 phos Tyr1007/1008
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Tyr 1007/Tyr 1008 of human JAK2
detection of dually phosphorylated Tyr 1007 and Tyr 1008 of JAK2
-
Jak2 phos 221
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Tyr 221 of human JAK2
detection of phosphorylated Tyr 221 of JAK2
-
Jak2
Novus Biologicals
polyclonal
IgG
Goat
(liquid, serum, TBS, BSA, azide, affinity purified)
-
Human
immunogen = synthetic peptide C-R V D Q V R D N M A G, representing the C-terminus of the human protein sequence according to AAC23653
JAK2
-
Jak2
Novus Biologicals
polyclonal
IgG
Goat
(liquid, serum, BSA, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide C-R V D Q V R D N M A G, representing the C-terminus of the human protein sequence according to AAC23653
detects endogenous levels of JAK2 protein.
-
Jak2 phos Y1007/Y1008 (E132)
Novus Biologicals
monoclonal
IgG
Rabbit
(liquid, ascites, Tris-glycine saline, BSA, azide, glycerol, purified)
-
Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr 1007 and Tyr 1008 of human JAK2
detects JAK2 phosphorylated on Tyrosine 1007 and 1008
-
Jak2 phos TYR1007
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, antiserum, azide, protein A purified)
-
Human, Mouse, Rat
-
detects endogenous levels of p-JAK2 (Y1007) protein
-
Jak2
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized non-phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 221 (Q-D-Yp-H-I)
detects endogenous levels of total JAK2 protein
-
Jak2 phos TYR1007
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 1007 (K-E-Yp-Y-K)
detects endogenous levels of JAK2 only when phosphorylated at tyrosine 1007
-
Jak2 phos TYR221
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthesized phosphopeptide derived from human JAK2 around the phosphorylation site of tyrosine 221 (Q-D-Yp-H-I)
detects endogenous levels of JAK2 only when phosphorylated at tyrosine 221
-
Jak2
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, glycerol, PBS, azide, affinity purified)
-
Human, Rat
immunogen = synthesized non-phosphopeptide derived from human Jak2 around the phosphorylation site of tyrosine 570 (G-D-Yp-G-Q)
detects endogenous levels of JAK2 protein.
-
Jak2 phos Tyr1007/Tyr1008
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic phospho peptide (human)
detects JAK2 phosphorylated on Tyrosine 1007 and 1008
-
AMPKα (F6)
Cell Signaling
monoclonal
IgG2b
Mouse
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
immunogen = purified recombinanthuman AMPKα1 protein
recognizes endogenous levels of total AMPKα, 62 kDa
-
AMPKα
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified)
-
Human, Monkey, Hamster, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled)derived from the amino-terminal sequence of humanAMPKα.
recognizes endogenous levels of AMPKα detects both the α-1 and α-2 isoforms of the catalytic subunit, butit does not detect the regulatory β or γ subunits
-
AMPKα, phos Thr172 (D79.5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat, S. cerevisiae, Drosophila
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingThr172 of human AMPKa
detects endogenous AMPKα only whenphosphorylated at threonine 172. The antibody detects bothα-1 and α-2 isoforms of the catalytic subunit, but does notdetect the regulatory β or γ subunits
-
AMPKα, phos Thr172 (D79.5E)
Cell Signaling
monoclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingThr172 of human AMPKa
detects endogenous AMPKα only whenphosphorylated at threonine 172. The antibody detects bothα-1 and α-2 isoforms of the catalytic subunit, but does notdetect the regulatory β or γ subunits
-
AMPKα1, phos Ser485 (45F5)
Cell Signaling
monoclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingSer485 of human AMPKα1
detects endogenous levels of AMPKα1only when phosphorylated at Ser485. The antibody does not cross-react with phosphorylated AMPKα2 or other relatedproteins
-
AMPKα1, phos Ser485
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingSer485 of human AMPKα1
detects endogenous levels of AMPKα1only when phosphorylated at Ser485. The antibody does not cross-react with phosphorylated AMPKα2 or other relatedproteins
-
AMPKα1, phos Ser485 and AMPKα2, phos Ser491
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol, affinity purified)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingSer491 of human AMPKa2.
recognizes endogenous levels ofAMPKα1/α2 only when phosphorylated at serine 485 or serine 491. This antibody does not cross-react with otherrelated proteins
-
PAK1+2+3 phos Thr402
Novus Biologicals
polyclonal
IgG
Rabbit
(affinity pruified rabbit antisera, Hepes, saline, BSA, glycerol)
-
Primate (various), Human, Mouse, Rat
immunogen = Phosphopeptide corresp. to amino acid residues surrounding the phospho-Thr402 of p21 Activated Kinase 2
detects PAK phos at Thr402, a band ~68-70 kDa in WB
-
PAK1 (EP656Y)
Novus Biologicals
monoclonal
IgG
Rabbit
(rabbit ascites, Tris-glycine, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresponding to internal residues in the human PAK1 protein
detects PAK1
-
Dlg /discs-large (4F3)
DSHB U. Iowa
monoclonal
IgG1 ,κ
Mouse
-
Drosophila
immunogen = Discs-Large PDZ2 domain
recognizes Dlg
-
PAK1
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Human, Monkey, Guinea Pig, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) corresp. to the amino-terminus of human PAK1
detects endogenous levels of total PAK1 protein; does not react with PAK2, PAK3 or other PAK family members.
-
PAK1/2/3
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
-
Human, Monkey, Guinea Pig, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) corresp. to residues at the carboxy-terminus of human PAK1
detects endogenous levels of PAK1, PAK2 and PAK3 protein; does not react with PAK4, PAK5 or PAK6.
-
JNK/SAPK phospho
A B R - Thermo Scientific
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = phosphorylated peptide (pT183-P-pY185) based on human JNK/SAPK
detects Phospho-JNK/SAPK
-
JNK/SAPK phospho
A B R - Thermo Scientific
polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = phosphorylated peptide (pT183-P-pY185) based on human JNK/SAPK
detects Phospho-JNK/SAPK
-
JNK/SAPK
A B R - Thermo Scientific
polyclonal
IgG
Rabbit
(liquid, PBS (without Mg2+ and Ca2+), saline, azide, glycerol)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresponding to human SAPK/JNK, around the phosphorylation site of tyrosine 185(T-P-Y-P-V-V)
detects JNK/SAPK
-
EphA4
Upstate and Chemicon are Millipore
polyclonal
Rabbit
(liquid, (NH4 )SO2 precipitated rabbitIgG, glycerol, BSA, azide)
-
Mouse, Rat
immunogen = GST EphA4 fusion protein corresp. to the SAM domain and C-terminal tail(amino acids 909 to 987) of mouse EphA4.
Recognizes EphA4 tyrosine kinasereceptor at 120kDa, and degradation products at95kDa and 50kDa
-
AMPKα1 (D-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping within an internal region of AMPKα1
AMPKα1
-
AMPKα1/2 phos Thr172
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope corresp. to phosphorylated Thr 172 of AMPKα2
detection of Thr 172 phosphorylated AMPK α1 and α2 isoforms
-
AMPKα1 (71.54)
Santa Cruz
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
-
Human
immunogen = recombinant human AMPKα1
AMPKα1
-
AMPKα1/2 (H-300)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa 251-550 mapping at the C-terminus of human AMPKα1
detection of AMPK α1 and α2 isoforms
-
AMPKα1/2 (D-6)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
-
Human, Mouse, Rat
immunogen = epitope corresp. to aa 251-550 mapping at the C-terminus of human AMPKα1
detection of AMPK α1 and α2 isoforms
-
AMPKγ (Y307)
Abcam
monoclonal
IgG
Rabbit
(liquid, Tris glycine sa;ine, BSA, glycerol, azide, protein A purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide corresp. to residues from the N-terminus of human AMPK gamma 1 subunit
reacts with an epitope located in the N-terminal region of AMPK gamma 1
-
AMPKα1 (C-20)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
-
Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human AMPKα1
AMPKα1
-
JNK, paired183/185
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse
immunogen = a synthetic peptide corresponding to the sequence around Thr183 and Tyr185 of human JNK (MMTPYVVT)
detects JNK
-
JNK, phos Thr183/Tyr185
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide corresponding to the phospholyted human JNK at Threonine 183 and Tyrosine 185 (MM-pT-P-pY-VVT)
detects JNK phos at Thr183 and Tyr185
-
JNK-1/2/3, CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, MOPS, BSA, glycerol)
-
Zebra fish
immunogen = a synthetic peptide corresponding to C-terminal (CT) region of zebrafish JNK-1
detects JNK
-
JNK, CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide corresponding to the C-terminal portion of human JNK
detects JNK
-
JNK, NT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Chicken, Human, Monkey, Mouse, Rat
immunogen = a synthetic peptide corresponding to the N-terminal portion of human JNK
detects JNK
-
JNK, NT-1
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse
immunogen = a synthetic peptide corresponding to the N-terminal portion of human JNK1
detects JNK
-
PAK4 CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide corresponding to the amino acids 470-485 of human PAK4
detects PAK4
-
PAK-α
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the amino acids 525-545 of rat PAK-α
detects PAK-α
-
PAK2 CT
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = a 14 amino acid peptide from near the C-terminus of human PAK2
detects PAK2
-
PAK4 phos Ser474
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (RRKpSLVG) corresponding to human PAK4 with a phosphorylated site at Serine 474
detects PAK4
-
PAK-γ
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
-
Human, Mouse, Rat
immunogen = a synthetic peptide corresponding to the N-terminus of human PAK-γ protein
detects PAK-γ
-
PAK4 phos Ser474
Anaspec
polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
-
Human
immunogen = a synthetic peptide (RRKpSLVG) corresponding to human PAK4 with a phosphorylated site at Serine 474
detects PAK4
-
PAK6
Anaspec
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
-
Human
immunogen = a 13 amino acid peptide from near the center of human PAK6
detects PAK6
-
PAK5
Anaspec
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
-
Human
immunogen = a 13 amino acid peptide from near the center of human PAK5
detects PAK5
-
PAK4 IN
Anaspec
polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
-
Human
immunogen = a 13 amino acid peptide from near the center of human PAK4
detects PAK4
-
CASK (K56A)
Chemicon and Upstate are Millipore
monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
-
Human, Mouse, Rat
Immunogen = CASK-GST fusion protein (aa318-415)
CASK
-
GRK2 (C-15)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatinAvailable as agarose (sc-562 AC) conjugate for IP)
-
Bovine, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 2 of human origin
for detection of GRK2
-
GRK3 (C-14)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatin)
-
Cow, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 3 of bovine origin
for detection of GRK3
-
GRK6 (C-20)
Santa Cruz
polyclonal
IgG
Rabbit
(liquid, affinity purified IgG, PBS, azide, gelatinAvailable as agarose conjugate for IP, sc-566 AC,)
-
Bovine, Human, Mouse, Rat
immunogen = a peptide mapping at the C-terminus of GRK 6 of human origin
for detection of GRK6
-
CK-IIα' (C-20)
Santa Cruz
polyclonal
IgG1
Goat
(liquid, PBS, azide, gelatin, affinity purified Also available as agarose conj. for IP, Cat# sc-6481 AC)
-
Bovine, Human, Mouse, Rat
immunogen = epitope mapping at the C-terminus of human Casein Kinase IIα '
detects Casein Kinase IIα'
-
CK-2β (6D5)
Sigma Aldrich
monoclonal
IgG1
Mouse
(liquid, PBS, azide)
-
Bovine, Human, Hamster, Mouse, Rat
immunogen = recombinant human casein kinase 2β (CK2β). (the antibody epitope resides within amino acids 123-127)
recognizes Casein Kinase 2β
-
CK-2α
Pierce Thermo Scientific
monoclonal
Mouse
-
Bovine, Hamster, Mouse, Rat
immunogen = denatured purified human recombinant CK2, alpha subunit
reacts with Casein Kinase 2 alpha-subunit
-
AMPKα, phos Thr172
Abcam
polyclonal
IgG
Rabbit
(liquid, antiserum, affinity purified, glycerol, PBS, saline, azide)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptidederived from human AMPK alpha 1 around the phosphorylation site of threonine 172 (L-R-phosphoT-S-C)
detects endogenous AMPKα only whenphosphorylated at SDGEFLR(pT)SCGSPNY. The antibody detects both alpha1 and alpha2 isoforms
-
AMPKα, phos Thr172 (40H9)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Hamster, Mouse, Rat, S. cerevisiae, Drosophila
immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingThr172 of human AMPKα
detects endogenous AMPKα only whenphosphorylated at threonine 172. The antibody detects bothα-1 and α-2 isoforms of the catalytic subunit, but does notdetect the regulatory β or γ subunits
-
p70 S6 Kinase total (49D7)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase total (49D7)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol.Also available Biotinylated, Cat# 5707)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide corresponding to residues surrounding the amino-terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase total
Cell Signaling
polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
-
Human, Monkey, Mouse, Rat
immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding the carboxy terminus of human p70 S6 kinase
detects endogenous levels of total p70 S6 kinase protein. This antibody also recognizes p85 S6 kinase
-
p70 S6 Kinase phos Thr389 (1A5)
Cell Signaling
monoclonal
IgG2a
Mouse
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues around Thr389 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at Thr389; also detects p85 S6 kinase when phosphor. at the analogous site (Thr412) and possibly S6KII phosphor. at Thr401
-
BRSK1
Acris Antibodies
polyclonal
Rabbit
(liquid, prrified Ig fraction, PBS, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = a 28 amino acid peptide from near the center of humanBRSK1
recognizes BRSK1
-
JNK1+2, phos T183/Y185
Abcam
polyclonal
IgG
Rabbit
(liquid, affinity purified, glycerol, BSA, PBS, azide)
-
Human, Mouse
immunogen = Synthetic peptide (Human) derived from the region of JNK 1 + 2 that contains threonine 183 and tyrosine 185, based on the human sequence (conservd among many species)
detects JNK 1 + 2 phosphorylated at threonine 183 and tyrosine 185
-
CASK (7)
BD Transduction
monoclonal
IgG1
Mouse
(liquid, affinity purified)
-
Dog, Frog, Human, Mouse, Rat
Immunogen = aa 353 to 486 from rat CASK
detects CASK
-