Cdc2-CRK7
Novus Biologicals
polyclonal
Rabbit
(liquid, serum, PBS, azide)
-
Human
immunogen = synthetic peptide made to the internal region of cdc2-CRK7
CDC2-related protein kinase 7 (CRK7)
-
Cdc2L5 (1B3-1B11)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = CDC2L5 (AAH01274, 1 a.a. ~ 325 a.a) full length recombinant protein with GST tag
CDC2L5 - cell division cycle 2-like 5 (cholinesterase-related cell division controller)
-
Cdc2L5 (3G1)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = paartial recombinant CDC2L5
CDC2L5 - cell division cycle 2-like 5 (cholinesterase-related cell division controller)
-
CDK2 (2E8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDK2
CDK2 - cyclin-dependent kinase 2
-
CDK2 (3A2-3G6)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = full length recombinant CDK2
CDK2 - cyclin-dependent kinase 2
-
CDK2
Novus Biologicals
polyclonal
Mouse
(liquid, serum, glycerol, no preservative)
-
Human
immunogen = full length recombinant CDK2.
CDK2 - cyclin-dependent kinase 2
-
CDK2
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, sterile filtered, azide)
-
Human, Rat
immunogen = synthetic peptide (human) conjugated to KLH
CDK2 - cyclin-dependent kinase 2; no reaction to other related cyclin dependent kinases
-
CDKL1 (1F12)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL1
CDKL1 - cyclin-dependent kinase-like 1 (CDC2-related kinase)
-
CDKL1 (2H6)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL1
CDKL1 - cyclin-dependent kinase-like 1 (CDC2-related kinase)
-
CDKL1 (3G8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL1
CDKL1 - cyclin-dependent kinase-like 1 (CDC2-related kinase)
-
CDKL1 (5B11)
Novus Biologicals
monoclonal
IgG2a , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL1
CDKL1 - cyclin-dependent kinase-like 1 (CDC2-related kinase)
-
CDKL2 (6H8)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL2
CDKL2 - cyclin-dependent kinase-like 2 (CDC2-related kinase)
-
CDKL2
Novus Biologicals
polyclonal
Mouse
(liquid, serum, glycerol, no preservative)
-
Human
immunogen = partial recombinant CDKL2
CDKL2 - cyclin-dependent kinase-like 2 (CDC2-related kinase)
-
CRKRS (2F6)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CRKRS
CRKRS - Cdc2-related kinase, arginine/serine-rich
-
CDKL1
Novus Biologicals
polyclonal
Mouse
(liquid, serum, glycerol, no preservative)
-
Human
immunogen = partial recombinant CDKL1
CDKL1 - cyclin-dependent kinase-like 1 (CDC2-related kinase)
-
CDKL2 (1F6)
Novus Biologicals
monoclonal
IgG1 , κ
Mouse
(liquid, purified, no preservative)
-
Human
immunogen = partial recombinant CDKL2
CDKL2 - cyclin-dependent kinase-like 2 (CDC2-related kinase)
-
Cdc2 phos Tyr15
Phospho- solutions
polyclonal
Rabbit
(serum, affinity purified)
-
Human, Xenopus
immunogen = phospho peptide corresp. to aa residues surrounding the phospho Tyr15 of rat cdc2
Specific for the ~38k cdc2 protein phosphor. at Tyr15
-
Cdk5(p31)
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, filtered, azide)
-
Human, Mouse, Rat
immunogen = peptide corr. to C-terminus of human Cdk5 (p31) conj. to KLH
35kDa Cdk5 (p31), not other Cdk's
-
Cdk4
Biotrend
polyclonal
Rabbit
(liquid, IgG fraction)
-
Bovine, Dog, Porcine, Sheep, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat
-
cdk4
-
Cdk4
Biotrend
polyclonal
Rabbit
(liquid, IgG fraction)
-
Bovine, Dog, Porcine, Sheep, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat
-
cdk4
-
Cdk4
Biotrend
kit
Unknown Host
(detection and purification kit1, 96T)
-
Human
-
detects cdk4
-
Cdk5 (DC17 + DC34)
Biotrend
monoclonal
IgG1
Mouse
(liquid, IgG fraction)
-
Mouse, Rat
peptide corresponding to the C-terminus of the human protein conjugated to KLH
cdk5
-
Cdk5
Biotrend
monoclonal
IgG1
Mouse
(liquid, IgG fraction)
-
Mouse, Rat
-
recognizes cdk5
-
Cdk4
Rockland
polyclonal
Rabbit
(liquid, azide, delipidized, unconjugated)
-
Human, Mouse, Rat
immunogen = cdk4 peptide corresp. to the C-terminus of the human protein, conj. to KLH
cdk4, not related Cdk's
-
Cdk4 Inhibitor /p16
Rockland
polyclonal
Rabbit
(liquid, azide, no buffers or stabilizers)
-
Human
immunogen = human p16 protein (missing the first 7 amino acids)
will specifically react with a p16/CDK4I protein from human tissue. Cross reactivity may occur with p15 when immunoprecipitating in vitro translated product but not in immunoblots
-
Cdk5 (p31)
Rockland
polyclonal
Rabbit
(liquid, azide, delipidized)
-
Human, Mouse, Rat
immunogen = peptide corresp. to the C-terminus of the human protein, conj. to KLH
35 kDa cdk5, not related Cdk's
-
Cdk4 (DCS-31.2)
AbD Serotec
monoclonal
IgG1
Mouse
(liquid, PBS, azide, purified)
-
Human, Mouse, Rat
immunogen =recombinant full length protein (human)
specifically with Cdk4; not other Cdk types; does not co-precipitate the D type cyclins bound to cdk4
-
Cdk4 (4F11)
AbD Serotec
monoclonal
IgG
Mouse
(liquid, affinity purified, PBS, no preservative or stabilizers)
-
Human, Mouse, Rat
immunogen =recombinant protein corresponding to aa 211 - 304 of human CDK4
specific for human Cdk4
-
Cdk5
AbD Serotec
polyclonal
IgG
Rabbit
(serum, liquid, azide)
-
Human, Mouse, Rat
immunogen =peptide corresp. to C-terminus of human Cdk5, conjugated to KLH
detects human cyclin dependent kinase 5 (Cdk5)
-
Ki-67
Novocastra
polyclonal
Ig
Rabbit
(serum, azide, lyophilized)
-
Human, Mouse, Rat
immunogen = prokaryotic recombinant fusion protein corr. to 1086bp Ki67 motif-containing cDNA fragment
Ki67 nuclear antigen expressed on all proliferating cells during late G1, S, M andG2 phases of the cell cycle
-
Ki-67 (MM1)
Novocastra
monoclonal
IgG1
Mouse
(liquid, supernat, azide)
-
Human, Mouse, Rat
immunogen = prokaryotic recombinant fusion protein corr. to 1086bp Ki67 motif-containing cDNA fragment
Ki67 nuclear antigen expressed on all proliferating cells during late G1, S, M andG2 phases of the cell cycle
-
Ki-67 (MM1)
Novocastra
monoclonal
IgG1
Mouse
(supernat, azide, lyophilized)
-
Human, Mouse, Rat
immunogen = prokaryotic recombinant fusion protein corr. to 1086bp Ki67 motif-containing cDNA fragment
Ki67 nuclear antigen expressed on all proliferating cells during late G1, S, M and G2 phases of the cell cycle
-
Ki-67 (MM1)
Novocastra
monoclonal
IgG1
Mouse
(liquid, supernatant, PBS, azide, predilute)
-
Human, Mouse, Rat
immunogen = prokaryotic recombinant fusion protein corr. to 1086bp Ki67 motif-containing cDNA fragment
Ki67 nuclear antigen expressed on all proliferating cells during late G1, S, M andG2 phases of the cell cycle
-
Src, pan
Biosource Invitrogen
polyclonal
IgG1
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
immunogen = a chemically synthesized peptide derived from the amino acid region 31-49 of human Src protein
detects pan Src (31-49)
-
Src (EG107)
Abcam
monoclonal
IgG
Rabbit
(liquid, glycerol, BSA, saline, Tris-glycine, azide)
-
Human, Mouse
immunogen = a synthetic peptide corresponding to residues to the C terminus of human Src.
detects Src
-
PCNA (PC10)
Zymed/ Invitrogen
monoclonal
IgG2a
Mouse
(liquid, purified ascites, PBS, azide)
-
Insect, Various vertebrate, S. pombe
immunogen = rat recombinant PCNA polypeptides
reacts with PCNA (36 kDa)
-
PCNA (PC10)
Invitrogen Corporation
monoclonal
IgG2a
Mouse
(liquid, PBS, azide, BSA added for conjugated versions)
-
Insect, Various vertebrate
immunogen = rat recombinant PCNA
detects PCNA (36 kDa)
-
Cdc2
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, serum, no buffer, azide)
-
Human, Mouse, Rat
immunogen = synthetic peptide (human) conjugated to KLH
detects CDC2; no reaction to other related cyclin dependent kinases; inhibits activation of p34cdc2 kinase by cyclins
-
Cdc2
Novus Biologicals
polyclonal
IgG
Rabbit
(liquid, IgG purified, PBS, azide)
-
Human, Mouse, Rat
immunogen = C-terminal synthetic peptide (human)
detects CDC2 p34
-
PCNA (PC10)
Zymed/ Invitrogen
monoclonal
IgG2a
Mouse
(liquid, PBS, glycerol, BSA, azide, Biotin conj.)
-
Chicken, Insect, Various vertebrate, Human, Mouse, Rat, Xenopus, S. pombe
immunogen = recombinant PCNA polypeptides
reacts with PCNA (36 kDa)
-
Rac1
Proteintech Group, Inc.
polyclonal
Rabbit
(liquid, affinity purified, PBS, glycerol, azide)
-
Human, Mouse
Immunogen = ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
recognizes Rac1
-
Sestrin 2
Proteintech Group, Inc.
polyclonal
Rabbit
(liquid, affinity purified, PBS, azide, glycerol)
-
Human, Mouse
immunogen = recombinant SES2
detects SES2, 54kDa
-
Ki-67 (MIB-1)
DAKO
monoclonal
IgG1 ,κ
Mouse
(liquid, supernatant, Tris/HCl, BSA, azide;also available purified, FITC conj:Cat# F7268)
-
Mammal (various), Human
immunogen = human recomb. peptide corresp. to a 1002 bp Ki-67 cDNA fragment
labels 345 and 395 kDa Ki-67; recognizes native Ki-67 antigen and recombinant fragments of the Ki-67 molecule
-
Ki-67 (Ki-67)
DAKO
monoclonal
IgG1 κ
Mouse
(liquid, purified, BSA, saline, azide, FITC conj.)
-
Human
immunogen = crude nuclear fraction of L428 cells
Ki-67 in cells in the proliferative cycle, but not quiescent cells
-
Ki-67 (MIB-1)
DAKO
monoclonal
IgG1 ,κ
Mouse
(liquid, buffered, stabilizing protein, azide, ready-to-use)
-
Human
immunogen = human recomb. peptide corresp. to a 1002 bp Ki-67 cDNA fragment
labels 345 and 395 kDa Ki-67
-
Ki-67 (MIB-1)
DAKO
monoclonal
IgG1 ,κ
Mouse
(liquid, buffered, stabilizing protein, azide, ready-to-use Dako Autostainer /Autostainer Plus)
-
Human
immunogen = human recomb. peptide corresp. to a 1002 bp Ki-67 cDNA fragment
labels 345 and 395 kDa Ki-67
-
Ki-67 (TEC-3)
DAKO
monoclonal
IgG2a
Rat
(culture supernatant, Tris, saline, azide)
-
Mouse
immunogen = parts of mouse Ki-67-equivalent cDNA bacterially expressed as recomb. protein
Ki-67 in mouse cells
-
Ki-67 (MIB-5)
DAKO
monoclonal
IgG1
Mouse
(liquid, supernatant, Tris/HCl, azide)
-
Mouse, Rat
immunogen = bacterially expressed parts of human Ki-67 cDNA
Ki-67; identifies cycling cells in embryonic and adult rat tissue
-
Ki-67 (PP-67)
Diagnostic Biosystems
monoclonal
IgM
Mouse
(liquid, diluted ascites, azide)
-
Non-specific
immunogen = a HeLa cell nuclear preparation
reacts with Ki-67; staining pattern similar to anti Ki-67/MIB-1 reagents
-
Ki-67 (MM1 /MB67)
Diagnostic Biosystems
monoclonal
IgG1 ,κ
Mouse
(tissue culture supernatant, azide)
-
Human
immunogen = prokaryotic recomb. protein corresp. to a 1086 bp of Ki-67 motif containing cDNA fragment
reacts with Ki-67
-
Ki-67
Diagnostic Biosystems
polyclonal
Rabbit
(purified Ig fraction of rabbit antiserum, azide, concentrated)
-
Mammal (various), Human
immunogen = synthetic peptide from the 62 base pair region of human Ki-67
reacts with Ki-67
-
Ki-67 (MM1 /MB67)
Diagnostic Biosystems
monoclonal
IgG1 ,κ
Mouse
(tissue culture supernatant, azide, Preclin 300, pretitered)
-
Human
immunogen = prokaryotic recomb. protein corresp. to a 1086 bp of Ki-67 motif containing cDNA fragment
reacts with Ki-67
-
Ki-67
Diagnostic Biosystems
polyclonal
IgG1
Rabbit
(liquid, pretitered, ready to use)
-
Human
immunogen = synthetic peptide from the 62 base pair region of human Ki-67
reacts with Ki-67
-
Ki-67 (SP6)
Diagnostic Biosystems
monoclonal
IgG
Rabbit
(liquid, pretitered)
-
Human, Rat
immunogen = a synthetic peptide from the C-terminus of human Ki-67
reacts with Ki-67
-
Ki-67 (SP6)
Diagnostic Biosystems
monoclonal
IgG
Rabbit
(liquid, tissue culture supernatant, azide, concentrated)
-
Human, Rat
immunogen = a synthetic peptide from the C-terminus of human Ki-67
reacts with Ki-67
-
TSG101 (4A10)
GeneTex
monoclonal
IgG1 ,κ
Mouse
(liquid, purified from ascitic fluid, PBS, no preservative)
-
Human, Mouse
immunogen = aa. 167-374 of TSG101 protein expressed in E. coli
46 kDa TSG101 protein (Tumor Susceptibility Gene 101)
-
PCNA (PC10)
Zymed/ Invitrogen
monoclonal
IgG2a
Mouse
(liquid, purified ascites, PBS, BSA, azide)
-
Primate (various), Insect, Yeast, Human, Mouse, Rat
immunogen = rat recombinant PCNA
reacts with PCNA (36 kDa)
-
PCNA (PC10)
Accurate
monoclonal
Mouse
(liquid, ascites.Also available purified FITC, RPE, HRP, prediluted, ready to use, supernatant, lyophilized supernatant;see List at Accurate Chemical)
-
Insect, Yeast, Various vertebrate, Human, Monkey
immunogen = primate Epithelial Cells,
reacts with PCNA (36 kDa)
-
PCNA (5A10)
Accurate
monoclonal
Mouse
(liquid, purified)
-
Human, Rat
-
reacts with PCNA (~36 kDa)
-
Src, phosY418
Biosource Invitrogen
polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purifiedAlso available Alexa Fluor® 488 for IF, Cat# 44-660A1)
-
Chicken, Human, Mouse
immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine)
detects Src; also reacts with Fyn and Yes
-
Src, phosY529
Biosource Invitrogen
polyclonal
IgG
Rabbit
(liquid Ig, Dulbecco’s PBS, glycerol, BSA, azide, affinity purified)
-
Chicken, Human, Mouse, Rat
immunogen = a chemically synthesized phosphopeptide derived from the region of human Src that contains tyrosine 529 (tyrosine 530 including the initiating methionine).
detects Src; not tested for reaction with Fyn and Yes
-
Fyn (15)
Santa Cruz
monoclonal
IgG1
Mouse
(liquid IgG, PBS, azide, gelatinAlso available as sc-434PE for FC, and sc-434AC for IP)
-
Human, Mouse, Rat
epitope mapping between amino acids 85-206 of Fyn p59 of human origin
detects Fyn p59
-
Fyn
Upstate and Chemicon are Millipore
polyclonal
IgG
Rabbit
(liquid, antiserum)
-
Avian (various), Human, Mouse, Rat
immunogen = synthetic peptide corresponding to aa 35-51 (TPQHYPSFG VTSIPNYN) of human Fyn
detects Fyn p59
-
Src phos Tyr416
Cell Signaling
polyclonal
Rabbit
(liquid, protein A and peptide affinity purified, HEPES, saline, BSA, glycerol)
-
Chicken, Human, Mouse, Rat, Xenopus
immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src
detects endogenous levels of Src only when phosphorylated at Tyr416; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites
-
Src phos Tyr416 (100F9)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, protein A and peptide affinity purified, HEPES, saline, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src
detects endogenous levels of Src only when phosphorylated at Tyr416; antibody may cross-react with other Src family members such as Yes, Fyn, Fgr and Yrk when phosphorylated at the equivalent sites
-
p35 (ES19, ES24)
Upstate and Chemicon are Millipore
monoclonal
IgG
Mouse
(frozen liquid, protein G purified, Tris-glycine, saline, azide)
-
Bovine, Human
immunogen = recombinant human p35
recognizes 33 kDa p35
-