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Antibodies

230 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Growth Factors From labs (1)
antibodies developed by W Stallcup, Reference: Ziskin et al,, 2007 polyclonal
Rabbit
IH - - PDGFαR -
Growth Factors From manufacturers (99)
BD Pharmingen monoclonal
IgG2a
Mouse
(liquid, aqueous, buffered, azide, affinity purified)
FC, IF, IH - immunogen = mouse PDGF Receptor α chain -human IgG1 fusion protein PDGFR α chain; binds to PDGF A and B chains -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRbeta PDGFR β chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified; conj. Biotin)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRβ PDGFR β chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified; conj. APC)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRβ PDGFR β chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified; conj. PE)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRβ PDGFR β chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRα PDGFR α chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified, conj. Biotin)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRα PDGFR α chain -
Biolegend monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified, conj. PE)
FC Human immunogen = NIH-3T3 cells transfected with human PDGFRα PDGFR α chain -
Biolegend polyclonal
IgG
Mouse
(liquid, affinity purified PBS, azide, glycerol)
IF, WB Human, Mouse, Rat immunogen = C- terminal peptide, KLH conj. recognizes EGF receptor -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, purified Ig, phosphate buffer, glycerol)
IH, IP Human, Mouse immunogen = human epidermal growth factor purified from urine reacts with EGF -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, PBS, stabilizers, Tween, ProClin, IHC Select®, ready to use)
IH Human immunogen = synthetic peptide from C-terminus of human EGFR reacts with EGF receptor -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, purified Ig, phosphate buffer, saline, glycerol, azide)
ELISA, IC, IH, WB Human immunogen = intracellular, activated EGFr Reacts strongly and specifically with the intracellular domain of the activated and phosphorylated EGFr; believed to recognize a phosphorylation-dependent conformational on the C-terminal domain -
Upstate and Chemicon are Millipore polyclonal
Rabbit
(lyophilized powder)
Neut, WB Mouse immunogen = receptor grade mouse EGF reacts with 6 kDa EGF -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(lyophilized, PBS, azide/PEG, sucrose)
IP, WB Human, Mouse immunogen = peptide containing the Y1173 motif (NAE[pY1173]LRV) of the human EGF receptor recognizes the motif NAE[pY]LRV corresp. to the major auto phosphorylation site of the human EGF receptor; this antibody does not interact with the non- phosphorylated EGF receptor -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, azide)
ELISA, FA, IP, RIA Human immunogen = native protein EGF (6kD) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
ELISA, Neut, WB Human immunogen = recombinant protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
IC, IP, Neut, WB Mouse - EGF (6kD) -
LifeSpan Biosciences, Inc. monoclonal
IgG2b
Mouse
(lyophilized, ion exchange chromatography)
ELISA, IP, WB Human immunogen = recombinant protein EGF -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(lyophilized, unpurified serum)
ELISA, IC, WB Human immunogen = synthetic peptide EGF; no reaction to IGF-1 (human), IGF-2 (rat), TGF-α (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), somatostatin and vasoactive intestinal peptide -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, purified)
ELISA, IC, WB Human immunogen = synthetic peptide EGF; no reaction to IGF-1 (human), IGF-2 (rat), TGF-α (human), ACTH (human, 1-39), Calcitonin (human), PTH (bovine, 1-84), somatostatin and vasoactive intestinal peptide -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(purified)
IC, WB Human immunogen = recombinant protein EGF; no reaction to TGF alpha, hIGF-1 and hIGF-2 -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS)
ELISA, FA, WB Human immunogen = recombinant human EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(lyophilized, affinity purified)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(lyophilized, affinity purified, conj. biotin)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
ELISA, WB Human immunogen = recombinant human EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
Goat
(lyophilized, affinity purified, conj. biotin)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(lyophilized, affinity purified)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(lyophilized, affinity purified, conj. biotin)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, ion exchange chromatography, conj. biotin)
ELISA, WB Human immunogen = recombinant human EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Sheep
(lyophilized, iaffinity purified)
ELISA, WB Rat immunogen = recombinant rat EGF protein EGF -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, supernatant)
ELISA, WB Human immunogen = native protein EGF; no reaction to TGFα, hIGF-1 and hIGF-2 -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, supernatant)
ELISA, WB Human immunogen = native protein EGF; no reaction to TGFα, hIGF-1 and hIGF-2 -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, purified)
ELISA, WB Human immunogen = native protein EGF; no reaction to TGFα, hIGF-1 and hIGF-2 -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(lyophilized, affinity purified, conj. biotin)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(liquid, PBS)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Goat
(liquid, PBS, conj. Biotin)
ELISA, WB Mouse immunogen = recombinant mouse EGF protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, conj. biotin)
ELISA, WB Human immunogen = recombinant human EGF protein EGF -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, thimerosal, glycerol, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant); no reaction with other cytokines TGFβ, IL-1B, IL-8, MCP-1 and SAA -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein G purified)
ELISA Human immunogen = recombinant human EGF protein EGF (natural and recombinant) -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, thimerosal, glycerol, affinity purified, conj. biotin)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant) -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified)
ELISA Human immunogen = recombinant protein EGF -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(lyophilized, affinity purified)
ELISA Human immunogen = recombinant protein EGF (natural and recombinant) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, Tris-HCl, affinity purified)
ELISA Human immunogen = recombinant protein recombinant EGF (oxidized or reduced form) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, Tris-HCl, affinity purified)
ELISA Mouse immunogen = recombinant protein recombinant EGF (oxidized or reduced form) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
ELISA Human - EGF -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(conj. biotin)
ELISA Human immunogen = recombinant protein EGF -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(azide, no preservative)
RIA Human, Mouse, Rat immunogen = native protein EGF native -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(no preservative)
RIA Mouse immunogen = native protein EGF native -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
IH Human immunogen = synthetic peptide C-terminal of human EGFR EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
IH Human immunogen = synthetic peptide C-terminal of human EGFR EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, serum)
ELISA, IH, IP, WB Human immunogen = synthetic peptide, C S L D N P D Y Q Q D F F P K E and corresp. to aa1166-1180 of the EGFR near the C-terminus identical in human, mouse and rat EGFR EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, KPBS, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic peptide aa1189-1199 of human EGFR EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, KPBS, azide, affinity purified)
ELISA, IH, WB Human immunogen = synthetic peptide aa1189-1199 of human EGFR EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified)
ELISA, FC, IF, IH, IP, WB Human immunogen = HC2 20 d2 cells; epitope maps to the extracellular domain of EGFR EGFR; recognizes the 170kD (wild type) and 145kD (VIII variant) forms -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, no preservative, no glycerol, affinity purified)
ELISA, FC, IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction other sites of tyrosine phosphorylation on EGF receptor; no cross-reaction with other tyrosine-phosphorylated proteins -
LifeSpan Biosciences, Inc. polyclonal
IgG
Sheep
(liquid, PBS, no preservative, glycerol, protein G purified)
ELISA, IC, IF, IP, WB Chicken, Human, Mouse, Rat immunogen = recombinant 20aa residues representing a portion of the internal domain of EGFR proximal to the phosphorylation region EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, no glycerol, affinity purified)
FC, IF, IH, IP, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other proteins of the ErbB family -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, no glycerol, affinity purified)
ELISA, IC, IF, IH, WB Human immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1045 of human EGF receptor, KLH coupled detects levels of EGF receptor only when phosphorylated at Tyr1045; may cross-react with other activated EGF receptor -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, glycerol, affinity purified)
ELISA, FC, IC, IF, IP, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr845 of human EGF receptor, KLH coupled detects endogenous levels of EGF receptor only when phosphorylated at Tyr845; may cross-react with other activated EGF receptor family members (e.g., ErbB2) but does not cross-react with other tyrosine-phosphorylated proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG
Mouse
(liquid, HEPES, NaCl, BSA, azide, glycerol)
FC, IC, IH, IP, WB Human immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled detects overexpressed levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other sites of tyrosine phosphorylation on the EGFR; no cross-react with other tyrosine-phosphorylated proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(lyophilized, affinity purified)
IC, IH, IP, Neut, WB Human immunogen = human A431 membrane proteins recognizes the external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells. Recognizes both phosphorylated and non-phosphorylated human EGF receptors -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, glycerol, purified)
ELISA, IC, IH, IP, WB Human immunogen = tyrosine-phosphorylated epidermal growth factor receptor reacts strongly and specifically with the intracellular domain of the activated and phosphorylated EGFR in human cell lines; no reaction with other phosphorylated proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, NaCl, azide)
FC, IH, IP, WB Human immunogen = A431 vulva epidermoid carcinoma cell line recognizes the EGF binding site of the extracellular domain of human EGFR (epidermal growth factor receptor; HER-1) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, glycerol, affinity purified)
ELISA, IC, IF, IH, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr992 of human EGF receptor, KLH coupled detects levels of EGF receptor only when phosphorylated at Tyr992 (175kDa); no cross-reaction with other phosphorylated receptor tyrosine kinases -
LifeSpan Biosciences, Inc. monoclonal
IgG
Rabbit
(liquid, Tris-glycine, NaCl, BSA, azide, glycerol)
ELISA, IF, IH, IP, WB Human immunogen = synthetic peptide corresp. to the aa residues including and surrounding tyrosine 1173 of the human EGF Receptor (EGFR) detects levels of EGF receptor only when phosphorylated at Tyr1173 -
LifeSpan Biosciences, Inc. monoclonal
IgG2a
Rat
(liquid, PBS, ion exchange chromatography)
ELISA, FC, IH, IP, WB Human immunogen = cells EGF receptor -
LifeSpan Biosciences, Inc. monoclonal
IgG2b, κ
Mouse
(liquid, PBS, BSA, azide, affinity purified)
ELISA, FC, IF, IH Equine, Human immunogen = A431 human epidermoid cells EGFR (170kD) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified)
IH, IP, WB Human immunogen = extracellular domain of human recombinant EGFR protein recognizes Epidermal Growth Factor Receptor (erbB, EGFR) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, protein G purified)
IH, IP, WB Human immunogen = extracellular domain of human recombinant EGFR protein; MW of antigen:170kD (wild type) and 145kD (vIII variant) recombinant EGFR protein; blocks the binding of EGF to EGFR -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH, IP, WB Human immunogen = synthetic peptide from the extracellular domain of human EGFR; MW of antigen: Wild Type: 170kD, vIII Variant: 145kD Epidermal Growth Factor Receptor (erbB, EGFR) -
LifeSpan Biosciences, Inc. monoclonal
IgG2a
Rat
(liquid, PBS, azide, ion exchange chromatography)
FC, IH, IP, WB Human immunogen = extracellular domain of human EGF-receptor from head and neck carcinoma Epidermal Growth Factor Receptor (erbB, EGFR) -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(lyophilized, purified, conj. PE)
ELISA, IC, IP, WB Dog, Human, Mouse immunogen = synthetic peptide containing the Y1173 motif (...N A E [pY 1173 ] L R V...) of the human EGF receptor, KLH-conjugated recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(lyophilized, purified, conj. PE)
ELISA, IC, IP, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(lyophilized, purified, conj. biotin)
ELISA, IC, IP, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(lyophilized, purified, conj. FITC)
ELISA, IC, IP, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E pY L R V) of EGFR (epidermal growth factor receptor) phosphorylated at Tyr1173 recognizes the motif N A E [pY] L R V corresp. to the major auto-phosphorylation site of EGFR; Does not recognize the non-phosphorylated EGFR or other unrelated phosphotyrosine proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
ELISA, IC, IP, WB Human, Mouse immunogen = synthetic peptide corresp. to aa 1170-1176 (N A E Y L R K) of EGFR (epidermal growth factor receptor) recognizes the motif N A E Y L R V corresp. to the major auto-phosphorylation site of EGFR; no cross-reaction with phosphorylated EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
ELISA, IC, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 1165-1185 of EGFR (epidermal growth factor receptor) recognizes the C-terminus (aa 1165-1186) of EGFR -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, no preservative, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to residues surrounding Tyr1068 of human EGF receptor, KLH coupled detects endogenous levels of EGF receptor only when phosphorylated at Tyr1068; no cross reaction with other sites of tyrosine phosphorylation on EGFR; no cross-reaction with other tyrosine-phosphorylated proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, azide, sucrose, glycerol, conj. to PE)
ELISA, IC, IP, WB Dog, Human, Mouse immunogen = synthetic peptide corresp. to a portion of human EGFR (epidermal growth factor receptor) phosphorylated at Ser1047 recognizes human EGFR phosphorylated at Ser1047; no cross-reaction with non-phosphorylated EGFR or other unrelated serine-phosphorylated proteins -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, azide)
FC, IF, IH, IP Human immunogen = HC2 20 d2 cells EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Rabbit
(liquid, PBS, Tris-glycine, NaCl, BSA, glycerol, azide, purified)
FC, IF, IP, WB Human immunogen = synthetic peptide corresp. to the C-terminal region of human EGF Receptor (EGFR) recognizes the C-terminal domain of EGFR and does not cross-react with any other ErbB family member -
LifeSpan Biosciences, Inc. polyclonal
Sheep
(liquid, protein G purified)
IC, IP, WB Chicken, Human, Rat immunogen = 20 aa synthetic peptide of human EGF receptor; sequence is proximal to the phosphorylation region (near the N-terminal sequence) recognizes the internal domain of the receptor molecule and will block the phosphorylation but not EGF -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified)
ELISA, IP, WB Equine, Human immunogen = synthetic peptide corresp. to residues 985-996 (D V V D A D E Y L I P Q) of the human EGF-Receptor recognizes the 170kD (wild type) and 145kD (vIII variant) forms of human EGF receptor. The epitope maps to the cytoplasmic domain of EGFR. Reacts with both the unphosphorylated and phosphorylated form of EGFR. Does not react with erbB-2, erbB-3 or erbB-4. -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, affinity purified)
ELISA, IH, IP Equine, Human immunogen = synthetic peptide corresp. to residues 985-996 (D V V D A D E Y L I P Q) of the human EGF-Receptor recognizes the 170kD (wild type) and 145kD (vIII variant) forms of human EGF receptor. The epitope maps to the cytoplasmic domain of EGFR. Reacts with both the unphosphorylated and phosphorylated form of EGFR. Does not react with erbB-2, erbB-3 or erbB-4. -
LifeSpan Biosciences, Inc. monoclonal
Rat
(liquid, PBS, azide, ion exchange chromatography)
IH, IP Human immunogen = squamous cell carcinoma EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG2b
Rat
(liquid, PBS, azide, ion exchange chromatography)
IH, IP Human immunogen = breast carcinoma cells EGFR; binds to epitope D -
LifeSpan Biosciences, Inc. monoclonal
IgG2a
Mouse
(liquid, Tris-glycine, NaCl, azide, affinity purified)
IC, IP, WB Human immunogen = A431 membrane proteins recognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kD -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, azide, glycerol, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide derived from the region of human EGFR that contains tyrosine 1173 EGFR phosphorylated at Tyr1173 -
LifeSpan Biosciences, Inc. polyclonal
IgG
Sheep
(liquid, PBS, azide, affinity purified)
IH, WB Human, Rat immunogen = synthetic peptide (Arg-Glu-lle-Pro-Asp-Leu-Leu-Glu-Lys-Gly-Glu-Arg-Leu-Pro-Gln-Pro-Pro-lle-Cys) from the cytoplasmic domain of human c-erbB-2 (KLH) EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, glycerol)
ELISA, IP, WB Human immunogen = extracellular and cytoplasmic domains of recombinant human EGFR protein EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG2a
Mouse
(liquid, PBS, BSA, azide, glycerol, affinity purified)
ELISA, IP, WB Human immunogen = extracellular domain of human recombinant EGFR protein, purified EGFR; (Mr 170kD and 145kD, corresponding to the intact and vIII variant); no cross-reactivity with c-erbB-2, c-erbB-3 or c-erbB-4. Epitope maps to the extracellular domain of EGFR (cell membrane) -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
ELISA, IH, WB Human immunogen = synthetic peptide from C-terminus of human EGFR protein EGFR -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(lyophilized)
ELISA, IP, WB Human, Mouse immunogen = synthetic peptide corresp. to EGFR (epidermal growth factor receptor) phosphorylated at Tyr1045, conj. to KLH detects EGF receptor only when phosphorylated at Tyr1045 -