Tools

Back to the Top

Antibodies

511 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Actin Related From manufacturers (100)
Cell Signaling polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
IH, IP, WB Human, Monkey, Mouse, Rat, Xenopus, Drosophila immunogen = synthetic peptide (KLH-coupled) corresp. to residues surrounding Asp244 of human beta-actin detects endogenous levels of total actin (all isoforms); also detected actin fragment (30kDa) cleaved at glutamate 107 -
Cell Signaling monoclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
FC, IC, IF, IH, WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the N-terminus of human beta-actin detects endogenous levels of total β-actin; may cross react with γ-actin; no cross reaction with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms -
Cell Signaling polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
IH, IP, WB Bovine, Mink, Chicken, Human, Monkey, Hamster, Mouse, Rat, Xenopus immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the N-terminus of human beta-actin detects endogenous levels of β-actin; may cross react with γ-actin; no cross reaction with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms -
BD Pharmingen polyclonal
Rabbit
(aqueous buffered solution, azide)
IF, IH, WB Human immunogen = human β-actin 240-245 Peptide recognizes the 32 kD cleaved fragment of human actin and does not recognize intact actin -
LifeSpan Biosciences, Inc. polyclonal
Chicken
(DEAE-column chromatography)
IC, IH, WB Human immunogen = synthetic peptide corresp. to aa1-375 β-actin -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(affinity purified)
IC, WB Human, Mouse, Rat immunogen = 16 aa peptide from near the N-terminus of human β-actin β-actin -
LifeSpan Biosciences, Inc. monoclonal
Mouse
(liquid, ascites)
IC, IF Bovine, Cat, Dog, Porcine, Rabbit, Chicken, Carp, Sheep, Human, Guinea Pig, Mouse, Rat, Xenopus immunogen = synthetic peptide(D D D I A A L V I D N G S G L) coupled to KLH, corresp. to aa 1-14 of Xenopus laevis beta isoform of actin β-actin; does not react with adult cardiac or skeletal muscle -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(DEAE-column chromatography)
IH, WB Bovine, Rabbit, Chicken, Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa 151-320 β-actin -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(protein G purified)
WB Human, Mouse, Rat immunogen = aa 2-16 (C D D D I A A L V I D N G S G) of actin protein β-actin -
LifeSpan Biosciences, Inc. polyclonal
Rabbit
(DEAE-column chromatography)
IC, IF, IP, WB Fish (various), Bovine, Cat, Dog, Rabbit, Chicken, Yeast, Sheep, Human, Mouse, Rat, Xenopus, Chinese hamster immunogen = synthetic peptide corresp. to aa 1-100 of human β-Actin β-actin; does not react with adult cardiac, smooth or skeletal muscle actin. -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, serum, no preservative)
IC, IH, WB Human, Mouse, Rat immunogen = K-Y E L P D peptide representing the last five aa of the C-terminus of the 32 kDa Fractin fragment of beta-Actin 32kDa Fractin fragment but not full length beta actin -
A B R - Thermo Scientific monoclonal
IgG
Mouse
(liquid, ascites, azide)
ELISA, WB Bovine, Zebra fish, Arabidopsis, Yeast, Sheep, Human, Mouse, Rat, Xenopus, Drosophila immunogen = purified Arabidopsis actin protein actin -
A B R - Thermo Scientific polyclonal
Rabbit
(liquid, Tris, BSA, azide, affinity purified antisera)
IP, WB Human, Mouse immunogen = N-terminus of human beta actin using the numbering given in Swiss-prot entry P60709 and is highly conserved with gamma actin 40 kDa Actin -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(liquid, prediluted)
IH, IP Human immunogen = synthetic peptide corresponding to the N-terminal residues of human beta actin β-Actin -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified IgG)
WB Human immunogen = recomb., full length human beta Actin β-Actin -
A B R - Thermo Scientific polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified IgG)
IH Human immunogen = synthetic peptide corresponding to the N-terminal residues of human beta actin β-Actin -
Sigma Aldrich polyclonal
IgG
Rabbit
(liquid, IgG fraction, PBS, azide)
IB, IF, IH Various species immunogen = N-terminal actin peptide (amino acid residues 20-33) attached to a multiple antigen peptide (MAP) backbone shows a broad reactivity among actin isoforms and across a range of organisms; specifically stains typical stress fibers in cultured cells using an indirect IF labeling technique and can be used as a probe of the N-terminal region of actin -
Sigma Aldrich monoclonal
IgG2a
Mouse
(liquid, ascites)
ELISA, IC, IH, WB Various species immunogen = synthetic actin C-terminal peptide, Ser-Gly-Pro-Ser-Ile- Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone 42 kDa actin; recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms -
Rockland polyclonal
Rabbit
(liquid, antiserum, sterile filtered, affinity purified, phosphate buffer, saline, azide)
ELISA, IH, WB Human - β-actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
FC, IF, IH, IP, WB Human, Mouse, Rat, Xenopus immunogen = peptide mapping at the C-terminus of human actin broad range of Actin isoforms -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ChIP, FC, IF, IP, WB Mammal (various), Dog, Zebra fish, Cow, Horse, Pig, Human, Mouse, Rat, Xenopus, C. elegans, S. cerevisiae immunogen = peptide mapping at the C-terminus of human actin broad range of Actin isoforms -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
FC, IF, IP, WB Human, Mouse, Rat immunogen = aa180-375 mapping at the C-terminus of human actin broad range of Actin isoforms -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
FC, IF, IP, WB Zebra fish, Human, Mouse, Rat, Xenopus, C. elegans, Drosophila immunogen = aa76-375 mapping at the C-terminus of human actin broad range of Actin isoforms -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
FC, IF, IP, WB Rabbit, Human, Mouse, Rat immunogen = monocytes and U-937 histolytic lymphoma of human origin Actin; no cross-reaction with the extreme N-terminal 40 aa -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
IF Human immunogen = K-562 human erythroleukemia cell line cytoplasmic Actin bundles -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, WB Chicken, Human, Mouse, Rat immunogen = purified chicken gizzard Actin Actin -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
FC, IF, IP, WB Human, Mouse, Rat immunogen = monocytes and U-937 histolytic lymphoma of human origin Actin; no cross-reaction with the N-terminal 40 aa -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
FC, IF, IP, WB Human, Mouse, Rat immunogen = monocytes and U-937 histolytic lymphoma of human origin Actin; no cross-reaction with the N-terminal 40 aa -
Santa Cruz monoclonal
IgM
Mouse
(liquid, supernatant, azide)
IF, WB Chicken immunogen = skeletal muscle-cell preparation of chicken origin Actin -
Santa Cruz monoclonal
IgM
Mouse
(liquid, supernatant, azide)
IF, WB Chicken immunogen = skeletal muscle-cell preparation of chicken origin Actin -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IH Human, Mouse, Rat immunogen = synthetic peptide corresp. to the N-terminal aa1-10 of human Actin Actin -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
IF, IH Rabbit, Human, Mouse, Rat immunogen = purified rabbit actin α-Actin; not Actin from fibroblasts (α-smooth muscle and β-cytoplasmic), aorta (α-smooth mucle) or chicken gizzard (γ-smooth muscle) -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = aa1-10 mapping at the N-terminus of smooth muscle α-Actin of human origin α-Actin; no cross reaction with Actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric) and myocardium (β-myocardial) -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = aa1-10 mapping at the N-terminus of human smooth muscle α-Actin α-Actin (smooth muscle) -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = aa1-10 mapping at the N-terminus of smooth muscle α-Actin of human origin α-Actin (smooth muscle) -
Santa Cruz monoclonal
IgM
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, WB Rabbit, Human, Mouse, Rat, Xenopus - α-skeletal and α-cardiac Actin; not α-Actin from smooth muscle tissue -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Chicken, Human, Mouse, Rat immunogen = purified chicken gizzard Actin pan-Actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Cow, Human, Mouse, Rat immunogen = cytoplasmic γ-Actin from cow brain tissue γ-Actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Cow, Human, Mouse, Rat immunogen = cytoplasmic γ-Actin from cow brain tissue γ-Actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Cow, Human, Mouse, Rat immunogen = cytoplasmic γ-Actin from cow brain tissue γ-Actin -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Mammal (various), Avian (various) immunogen = chicken gizzard actin α-smooth muscle, α-skeletal and α-cardiac (sarcomeric) isoforms of Actin; no cross-reaction with cytoplasmic actins -
Santa Cruz monoclonal
IgG2b
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Chicken, Human, Rat immunogen = chicken gizzard actin α- and γ- Actin isotypes of smooth muscle cells; no cross-reaction with skeletal or cardiac cells -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Mammal (various), Avian (various) immunogen = human vascular muscle Actin all muscle actins; no cross-reaction with cytoplasmic actins -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Human immunogen = human myocardium Actin muscle α and γ actin isomers; no cross-reaction with β-actin isomers -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
IF, IP, WB Human, Mouse, Rat immunogen = peptide mapping near the C-terminus of human Arp2 actin related protein 2 (Arp2) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
IF, IP, WB Human, Mouse, Rat immunogen = peptide mapping near the N-terminus of human Arp2 actin related protein 2 (Arp2) -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
IF, IH, IP, WB Human, Mouse, Rat immunogen = peptide corresp. to aa 311-394 mapping at the C-terminus of Arp2 actin related protein 2 (Arp2) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping near the N-terminus of Saccharomyces cerevisiae Arp2 actin related protein 2 (Arp2) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping near the N-terminus of Saccharomyces cerevisiae Arp2 actin related protein 2 (Arp2) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
IF, IH, IP, WB Mammal (various), Cow, Human, Mouse, Rat immunogen = peptide mapping near the C-terminus of human Arp3 actin related protein 3 (Arp3) -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = aa 1-110 of human Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
IF, IP, WB Mammal (various), Cow, Human, Mouse, Rat immunogen = peptide mapping near the N-terminus of human Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Human, Mouse, Rat immunogen = aa 1-110 mapping at the N-terminus of human Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
IF, IP, WB S. cerevisiae immunogen = aa 1-152 peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
IP, WB S. cerevisiae immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp3 actin related protein 3 (Arp3) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp9 actin related protein 9 (Arp9) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp9 actin related protein 9 (Arp9) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping at the N-terminus of Saccharomyces cerevisiae Arp7 actin related protein 7 (Arp7) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
WB S. cerevisiae immunogen = peptide mapping at the C-terminus of Saccharomyces cerevisiae Arp7 actin related protein 7 (Arp7) -
Abcam monoclonal
IgG1, κ
Mouse
(liquid,PBS, BSA, azide, protein G purified)
IC, IF, IH, IP, WB Dog, Rabbit, Chicken, Cow, Pig, Human, Mouse, Rat, Dictyostelium immunogen = chicken gizzard actin reacts with all six known vertebrate isoforms of actin; also reacts with two cytoplasmic actins (β, λ) -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, ascites, azide)
IC, IF, IH, WB Rabbit, Chicken, Cow, Sheep, Pig, Human, Mouse, Rat immunogen = chicken gizzard actin pan-actin -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, azide, protein A purified)
ELISA, IC, IH, WB Mammal (various), Avian (various), Bovine, Cat, Dog, Goat, Porcine, Rabbit, Rodent (various), Chicken, Frog, E. Coli, Protozoa, Yeast, Various species, Donkey, Horse, Sheep, Human, Guinea Pig, Hamster, Mouse, Rat, Xenopus, C. elegans, Macaque, Rhesus, Drosophila immunogen = chicken gizzard actin; Epitope appears to be located in the N-terminal two thirds of the actin molecule, possibly near aa 50-70 pan-actin that recognizes all six isoforms of vertebrate actin, globular (G) and fillimentous (F) forms; labels myotubes and stains myoblasts and fibroblasts. -
Abcam polyclonal
IgG
Rabbit
(liquid,PBS, BSA, glycerol, azide)
ELISA, IH Chicken, Human immunogen = actin isolated from chicken back muscle α actin; crossreacts with β, γ and δ actin; no crossreactivity with other cytoskeletal proteins -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH Human, Mouse immunogen = synthesized non phosphopeptide derived from human Actin pan (α/β/γ) around the phosphorylation site of tyrosine 53/55 endogenous levels of total actin protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH Human, Mouse, Rat immunogen = synthetic peptide derived from human Actin endogenous levels of total actin protein -
Abcam monoclonal
IgG2b
Rat
(liquid, PBS, azide, protein A purified)
EM, IC, IF Rat, Drosophila immunogen = flight muscle extract from Lethocerus indicus (Waterbug) Actin in flight and non-flight muscles of Lethocerus and Drosophila; reacts with actin in flight muscles of all other insect species tested -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
IC, IF, IH Dog, Horse, Pig, Human, Mouse, Rat immunogen = full length native protein (purified) reacts with α-smooth muscle, α-skeletal andα-cardiac (sarcomeric) isoform of actin. It is a pan muscle actin and reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, ammonium sulfate ppt)
IC, IF, WB Invertebrate (various), Chicken, Various vertebrate immunogen = purified full length native Actin protein from chicken gizzards reacts with F and G actins, from both muscle and non-muscle tissues -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified)
IC, IF, IH Dog, Chicken, Pig, Human, Mouse, Rat immunogen = full length purified human Actin reacts with α- smooth muscle as well as α-skeletal and α-cardiac (sarcomeric) isoform of actin. Reacts with tumors arising from smooth muscle (leiomyosarcomas) as well as skeletal muscle (rhabdomyosarcomas) -
Abcam monoclonal
IgM
Mouse
(liquid, ascites, thimerosal)
IH, WB Rabbit, Human immunogen = rabbit muscle α-actin crossreactive with actin in eukaryotic cells including β, γ and δ no reactivity with other cytoskeletal components -
Abcam monoclonal
IgG2a
Mouse
(liquid, ascites, azide; for conj. PBS, BSA, azide, protein A or G purified)
DB, ELISA, IC, ID, IF, IH, WB Dog, Goat, Rabbit, Chicken, Carp, Cow, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat immunogen = synthetic peptide: S G P S I V H R K C F, corresp. to C- terminal aa 365-375 of human Actin, attached to Multiple Antigen Peptide (MAP) backbone epitope located on the C-terminal end of actin, conserved in all actin isoforms; no reaction with the epitope on the N-terminal end -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, ascites, azide)
ID, WB Rabbit, Cow, Pig, Human immunogen = full length purified native cytosolic actin from Amoeba proteus actin epitope present in gizzard, skeletal and cardiac muscle -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, ascites, azide)
IH, WB Rabbit, Snake, Cow, Sheep, Human, Guinea Pig, Rat immunogen = N-terminal decapeptide of α-smooth muscle actin for 1A4 and with purified rabbit striated muscle actin for 5C5 actin from muscle cells -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide derived from human α-sarcomeric Actin endogenous levels of total γ-Actin (45kDa) -
Abcam monoclonal
IgG1
Mouse
(liquid, ascites, azide)
DB, ELISA, IC, IF, IH, IM, WB Fish (various), Cat, Dog, Rabbit, Chicken, Cow, Opossum, Sheep, Pig, Human, Guinea Pig, Hamster, Mouse, Rat, Drosophila immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. to aa1-14 of Xenopus laevis β-Actin, conj. to KLH β-Actin -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IC, IF, IH, WB Chicken, Cow, Pig, Human, Mouse, Rat immunogen = human synthetic peptide corresp. to N-terminus of smooth muscle α-actin α-smooth muscle actin;not β- and γ-cytoplasmic, striated muscle, and myocardium actins -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, supernatant, tris-borate, NaCl, azide)
ELISA, IC, IF, IH, WB Rabbit, Pig, Baboon, Human immunogen = N-terminal decapeptide of α-smooth muscle actin α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, affinity purified, conj. FITC)
FC, IH, WB Zebra fish, Human immunogen = N-terminal decapeptide of α-smooth muscle actin α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, PBS, BSA, protien A affinity purified, conj. FITC)
FC, IH, WB Rabbit, Zebra fish, Cow, Baboon, Human, Mouse, Rat immunogen = synthetic peptide: M C E E E D S T A L acetylated at the N-terminus, corresp. to aa 1-10 of α-smooth muscle Actin α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins -
Abcam monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, glycerol, azide)
FC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide: corresp. to human N-terminus α-smooth muscle Actin α-smooth muscle actin -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, conj. FITC)
IF, IH Fish (various), Cat, Dog, Rabbit, Chicken, Cow, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Drosophila immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. to aa1-14 of Xenopus laevis β-Actin, conj. to KLH β-Actin -
Abcam polyclonal
IgG
Rabbit
(liquid, BSA, Tris citrate/phosphate, azide, affinity purified)
IP, WB Human, Mouse immunogen = details of the immunogen are not available β and γ actin; epitope N-terminus of human γ actin -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IC, IF, WB Fish (various), Dog, Rabbit, Chicken, Zebra fish, Cow, Sheep, Human, Mouse, Rat, Xenopus, Rhesus, Chinese hamster immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin β-Actin -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, azide, affinity purified)
FC, IC, IF, WB Dog, Rabbit, Chicken, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, African green monkey, Chinese hamster immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin β-Actin; not adult cardiac, smooth or skeletal muscle actin -
Abcam monoclonal
IgG
Mouse
(liquid, PBS, azide, affinity purified, conj. HRP)
WB Dog, Rabbit, Chicken, Various species, Cow, Horse, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, S. cerevisiae, African green monkey, Chinese hamster, Drosophila immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH β-Actin -
Abcam monoclonal
IgG3, κ
Mouse
(liquid, Tris-HCl, NaCl, Na citrate. azide, protein A purified)
WB Cat, Dog, Rabbit, Chicken, Cow, Pig, Human, Mouse, Rat, Xenopus, S. cerevisiae, S. pombe, Chinese hamster, Drosophila immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH β-Actin (42kDa) -
Abcam monoclonal
IgM
Mouse
(liquid, PBS, azide, purified)
ELISA, FC, IH, WB Rabbit, Human, Rat immunogen = human monocytes and U397 cell line; epitope = located between residues 120 and 226 filamentous actin (F-actin); binds to the N-terminal region of actin, but not to the extreme N-terminal 40 aa. Stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IC, IF, WB Chicken, Cow, Human, Mouse, Rat, Xenopus, Drosophila immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human Actin, conj. to KLH β and λ actin; probably recognises all the other known forms of human actin -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
IH Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat immunogen = synthetic peptide of human N-terminal(unfortunately, the aa seq. is considered commercially sensitive) smooth muscle actin -
Abcam monoclonal
IgG
Rabbit
(liquid, tris-glycine saline, supernatant, BSA, glycerol, azide)
FC, IC, IF, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues near the N-terminus of human Actin muscle actin -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, PBS, BSA, azide, protein A purified)
IH Rabbit, Chicken, Cow, Baboon, Human, Mouse, Rat immunogen = synthetic peptide: M C E E E D S T A L, corresp. to N-terminal aa 1-10 of α-smooth muscle actin α-smooth muscle actin;not fibroblasts (β- and γ-cytoplasmic), striated muscle (α-saccomeric), and myocardium (α-myocardial) actins -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, thimerosal, protein A purified, conj. HRP)
WB Cat, Dog, Rabbit, Chicken, Frog, Carp, Sea Urchin, Various species, Cow, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus, Drosophila immunogen = synthetic peptide: D D D I A A L V I D N G S G L corresp. 100% to a sequence from: Xenopus borealis (Kenyan clawed frog), Lytechinus pictus (Painted sea urchin) and Engystomops pustulosus (Tungara frog), conj. to KLH β-Actin; not adult cardiac and skeletal muscle β-Actin -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, tris-borate saline, supernatant, azide)
IH Human immunogen = SDS extracted protein fraction of human myocardium muscle specific α and γ actin isomers; not β isomers -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, affinity purified)
IC, WB Rabbit, Chicken, Various species, Cow, Human, Mouse, Rat immunogen = aa 151-320 of human β-Actin β-Actin -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, BSA, azide, affinity purified)
IC, IF, WB Fish (various), Dog, Rabbit, Chicken, Cow, Human, Mouse, Rat, Xenopus, Chinese hamster immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin β-Actin (40kDa); no cross-reaction with adult cardiac, smooth or skeletal muscle actin -
Abcam monoclonal
IgG
Mouse
(liquid, supernatant, tris-borate, NaCl, azide)
IH, WB Human, Mouse, Rat immunogen = purified rabbit striated muscle actin α-skeletal and α-cardiac muscle actins; not smooth muscle tissue -
Abcam polyclonal
IgG
Goat
(liquid, PBS, azide, affinity purified)
IC, IF, WB Fish (various), Dog, Rabbit, Chicken, Various species, Cow, Human, Monkey, Mouse, Rat, Chinese hamster immunogen = synthetic peptide derived from within residues 1-100 of human β-Actin, conj. to KLH β-Actin -
Abcam polyclonal
IgG
Rabbit
(liquid, KPBS, azide, affinity purified)
ELISA, IH, WB Various species immunogen = synthetic peptide: T F Q Q M W I S K Q, corresp. to aa 359-368 of β-Actin β-Actin -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, azide)
EM, IC, IF, IH, WB Cat, Dog, Rabbit, Chicken, Carp, Cow, Sheep, Pig, Human, Guinea Pig, Mouse, Rat, Drosophila immunogen = synthetic peptide: D D D I A A L V I D N G S G L, conj. KLH β-Actin -