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Antibodies

67 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Protein Translation From labs (1)
from Nahum Sonenberg, Department of Biochemistry and McGill Cancer Center, McGill University, Montreal, paperReference: Giorgi, 2007 polyclonal
Rabbit
WB Human immunogen = peptide ATSGSARK RLLKEED, derivedfrom eIF4AIII, aa 8-22, conj. to KLH eIF4A III -
Protein Translation From manufacturers (66)
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified)
WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) derived from residues surrounding Phe52 of human eIF4A detects endogenous levels of total eIF4A1, eIF4A2 and eIF4A3 proteins -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
WB S. cerevisiae immunogen = peptide mapping near the N-terminus of eIF4A of Saccharomyces cerevisiaeorigin detects eIF4A -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = amino acids186-330 mapping within an internal region of eIF4AI of human origin detects eIF4AI and eIF4AII -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
IF, WB Human, Mouse, Rat immunogen = peptide mapping at the N-terminus of eIF4AI of human origin detects eIF4AI -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
IF, IP, WB Human, Mouse, Rat immunogen = peptide mapping at the N-terminus of eIF4AII of human origin detects 46 kDa eIF4AII -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Human immunogen = recombinantGST-tagged eIF4AIII protein of human origin detects eIF4AIII -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified)
IH, WB Zebra fish, Human, Monkey, Mouse, Rat immunogen = peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E detects endogenous levels of total eIF4E -
Biolegend polyclonal
IgG
Rabbit
(affinity purified, PBS, glycerol, azide)
WB Human immunogen = recombinant (partial), N-terminal recognizes human eIF-4E -
Cell Signaling polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
WB Human, Monkey, Mouse, Rat immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol, azideAvailable as Alexa Fluor® 488 Conjugate, #2846)
IC, IF, IH, WB Human, Monkey, Mouse, Rat, Drosophila immunogen = synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1 detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46; may cross-react with 4E-BP2 and 4E-BP3 when phosphor. at equivalent sites -
Cell Signaling polyclonal
Rabbit
(liquid, protein-A and affinity purified, HEPES, saline, BSA, glycerol)
IP, WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) corresponding to residues surrounding Ser112 of human 4E-BP1 detects endogenous levels of total 4E-BP1, independent of phosphorylation -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
WB Human, Monkey, Mouse, Rat immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α. detects endogenous levels of of total eIF2α protein -
Cell Signaling monoclonal
iGg
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
IH, IP, WB Human, Monkey, Mouse, Rat, Drosophila immunogen = synthetic peptide (KLH-coupled) derived from the carboxy terminal sequence of eIF2α. detects endogenous eIF2alpha only when phosphorylated at Ser51; does not recognize eIF2alpha phosphorylated at other sites -
Cell Signaling polyclonal
Rabbit
(liquid, affinity purified, sodium HEPES, BSA, glycerol)
WB Human, Monkey, Mouse, Rat immunogen = synthetic phospho peptide (KLH coupled) corresp. to residues surrounding Ser209 of eIF4E detects endogenous levels of eIF4E only when phosphorylated at serine 209 -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
IF, IH, IP, WB Human immunogen = raised against a recombinant protein corresp. to the N-terminal 118 aa of human 4E-BP1 4E-BP1 -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Pig, Human, Mouse, Rat immunogen = raised against full length human 4E-BP1 4E-BP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = mapping at the N-terminus of human 4E-BP1 4E-BP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide, affinity purified)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = mapping at the C-terminus of mouse 4E-BP1 4E-BP1 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1 detection of translational repressor 4E-BP1 and, to a lesser extent, PHAS-2 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1 4E-BP1 phosphorylated at Thr36 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa1-117 representing full length rat 4E-BP1 4E-BP1 phosphorylated at Ser65 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp.to a short aa seq. containing phosphorylated Ser 65 and Thr 70 of human 4E-BP1 4E-BP1 phosphorylated at Ser65 and Thr70 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 70 of human 4E-BP1 4E-BP1 phosphorylated at Thr70 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr69 of human 4E-BP1 4E-BP1 phosphorylated at Thr69 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa29-68 mapping within an internal region of human 4E-BP1 detects 4E-BP1, 4E-BP2 and /or 4E-BP3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
IF, IH, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 36 of human 4E-BP1/2/3 detects 4E-BP1, 4E-BP2 and /or 4E-BP3 phosphorylated at Thr 36 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1/2/3 detection of Thr 45 phosphorylated 4E-BP1/2/3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
IF, IH, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 45of human 4E-BP1/2/3 detection of Thr 45 phosphorylated 4E-BP1/2/3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to phosphorylated Thr 45 of human 4E-BP1 detection of Thr 45 phosphorylated 4E-BP1 and correspondingly phosphorylated 4E-BP2 and 4E-BP3 -
Abcam polyclonal
IgG
Rabbit
(liquid, Tris-citrate/phosphate, azide, affinity purified)
EMSA, WB Pig, Human, Mouse, Rat immunogen = synthetic peptide (human) - which represents a portion of human eIF-4EBP1 encoded in part by exons 2and 3 detects alpha (weakly), beta and gamma (strongly) isoforms of eIF-4EBP1 -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
ELISA, IH, WB Human immunogen = Ni-NTA purified truncated recombinant eIF4EBP1 expressed in E. coli strain BL21 (DE3) specific for elF4EBP1 -
Abcam monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol)
FC, IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C eIF4EBP1 -
Abcam monoclonal
IgG
Rabbit
(liquid, Tris-glycine saline, BSA, azide, glycerol)
FC, IH, IP, WB Human immunogen = synthetic peptide corresp. to residues near the C-term of human 4E-BP1 recognises 4E-BP1 (eIF4E-binding protein) also known as PHAS -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = Synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 64 (R-N-Sp-P-V) detects endogenous levels of eIF4EBP1 only when phosphorylated at serine 64 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic non phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) recognises endogenous levels of total eIF4EBP1 protein; may cross-react with 4E-BP2 and 4E-BP3 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of Threonine 70 (A K T P P) detects endogenous levels of total eIF4EBP1 protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic non-phosphopeptide derived from human eIF4EBP1 around the phosphorylation site of serine 65 (R-N-Sp-P-V) detects endogenous levels of total eIF4EBP1 protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
IC, IF, WB Human, Mouse immunogen = 14 aa synthetic peptide from near the C-terminus of human eIF4EBP1 eIF4EBP1 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH Human, Mouse, Rat immunogen = synthetic phosphopeptide derived from human eIF4EBP1 around the phosphorylation site threonine 70 (A-K-Tp-P-P) detects endogenous levels of eIF4EBP1 only when phosphorylated at threonine 69 -
Abcam monoclonal
IgG
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide)
IH, WB Human immunogen = a phospho specific peptide corresp. to residues surrounding threonine 70 of human eIF4EBP1 detects eIF4EBP1 phosphorylated at threonine 70 -
Abcam monoclonal
IgG
Rabbit
(liquid, BSA, supernatant, tris-glycine saline, glycerol, azide)
WB Human, Mouse immunogen = a phospho-peptide corresponding to residues surrounding threonine 37 of eIF4EBP1 detects eIF4EBP1 phosphorylated at threonine 37 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, glycerol, azide, affinity purified)
WB Human, Mouse immunogen = synthetic phosphopeptide derived from the region of human eIF4EBP1 that contains threonine 46; seq. is conserved in human, mouseand rat recognises eIF4EBP1 that is phosphorylated at threonine 46 -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
WB Human, Mouse, Rat immunogen = recombinant human eIF4EBP1 protein expressed in E. coli specific for elF4EBP1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat - detects endogenous levels of 4E-BP1 protein -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, gelatin, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat - detects endogenous levels of 4E-BP1 protein -
Novus Biologicals monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
FC, IH, IP, WB Human immunogen = synthetic peptide corresp. to residues near the C-term of human4E-BP1 4E-BP1 -
Novus Biologicals monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
FC, IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues near the N-term of human eIF4EBP1 (aa16-33): P A T R R V V L G D G V Q L P P G D C 4E-BP1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide)
WB Human, Mouse immunogen = human 4E-BP1 / initiation factor 4E binding protein 1 peptide 4E-BP1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, antiserum, PBS, azide, glycerol, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthesized non-phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) detects endogenous levels of total 4E-BP1 protein; may cross-react with 4E-BP2 and 4E-BP3 -
Novus Biologicals monoclonal
IgG
Mouse
(liquid, ascites, azide)
ELISA, WB Human immunogen = Ni-NTA purified truncated recombinant 4EBP1 expressed in E. coli strain BL21 (DE3) 4E-BP1 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat - detects endogenous levels of 4E-BP1 phosphorylated at Ser64 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IH Human, Mouse, Rat - detects endogenous levels of 4E-BP1 phosphorylated at Thr69 -
Novus Biologicals monoclonal
IgG
Rabbit
(liquid, ascites, tris-glycine, NaCl, BSA, glycerol, azide)
WB Human, Mouse immunogen = phospho-specific peptide corresp. to residues surrounding serine 65 of human 4E-BP1 detects 4E-BP1 phosphorylated at serine 65 -
Novus Biologicals monoclonal
IgG
Rabbit
(liquid, ascites, azide)
IH, WB Human immunogen = phospho-specific peptide corresp. to residues surrounding threonine 70 of human 4E-BP1 detects 4E-BP1 phosphorylated at threonine 70 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 36 (S-T-Tp-P-G) detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 36; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthesized phosphopeptide derived from human 4E-BP1 around the phosphorylation site of threonine 45 (S-T-Tp-P-G) detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 45; may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites -
EMD Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative)
IB, WB Zebra fish, Human, Monkey, Mouse, Rat, Drosophila immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr37 and Thr46 phosphorylation sites of mouse 4EBP1, coupled to KLH recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr37 and Thr46 -
EMD Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, glycerol, no preservative)
IB, IP, WB Human, Mouse, Rat, Drosophila immunogen = synthetic phosphopeptide corresp. to aa surrounding the Thr70 phosphorylation sites of rat 4EBP1 recognizes the ~20 kDa 4EBP1 protein, phosphorylated at Thr70 -
EMD Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, BBS, EDTA, NaCl, no preservative)
EMSA, IB, WB Human, Mouse, Rat immunogen = synthetic peptide (S P E D K R A G G E E S Q F E M D I) corresp. to aa101-118 of human PHAS-I, conjugated to KLH recognizes the ~19-25 kDa multiple phosphorylation states of native and recombinant PHAS-I protein -
AnaSpec polyclonal
IgG
Rabbit
(liquid, purified, PBS, azide)
ELISA, IC, WB Human, Mouse immunogen = 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1 dertects 4E-BP1 -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, purified, Tris-glycine, saline, azide)
IP, WB Mammal (various), S. cerevisiae immunogen = Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography recognizes EF-1α (Elongation factor-1 alpha), Mr 53 kDa -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = apeptide mapping at the N-terminus of eIF3η of human origin detects eIF3η -