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Antibodies

63 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Oligodendrocyte Markers/Myelin From labs (2)
antibodies developed by W. Stallcup, paperReference:Ziskin, 2007,Kukley, 2007 polyclonal
Rabbit
IH - - NG2 -
antibodies developed by W. Stallcup, paperReference:Ziskin, 2007,Kukley, 2007 polyclonal
Guinea Pig
IH - - NG2 -
Oligodendrocyte Markers/Myelin From manufacturers (61)
Chemicon and Upstate are Millipore monoclonal
IgM
Mouse
(liquid, purified Ig, phosphate buffer, azide)
IC, IH Chicken, Human, Mouse, Rat immunogen = homogenate of white matter of corpus callosum from bovine brain Anti-O4 can be used in myelination, demyelination and remyelination studies and in regeneration experiments -
SMI™ COVANCE monoclonal
IgG1
Mouse
(liquid, ascites, high titer)
ELISA, IH, WB Bovine, Dog, Sheep, Pig, Human, Mouse, Rat - reacts with the 46 and 48 Mr subunit of the 94 Mr myelin CNPase dimer; detects developing and adult myelin, developing oligodendrocytes and Schwann cells -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, protein-G purified Ig, phosphate buffer, saline, azide)
ELISA, IH, WB Rat immunogen = cell line expressing a truncated form of NG2 recognizes the >280 kDa NG2 protein; reacts with native, recombinant and protein from cells which express NG2 -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, ascites fluid, no preservative)
FC, WB Human immunogen = human bone marrow stromal cell line, SV40 transformed reacts with a 220-240 kDa surface protein whose N-terminal sequence is identical to the rat NG2 chondrotin sulfate proteoglycan molecule -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, supernatant, purified, Tris, fetal calf serum, azide)
ELISA, IH, RIA, WB Bovine, Human, Rat immunogen = bovine myelin basic protein (epitope at aa 129-138) recognizes MBP -
Chemicon and Upstate are Millipore monoclonal
IgG
Mouse
(liquid, PBS, glycerol, azide, protein-A purified)
FA, WB Human immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected -
Chemicon and Upstate are Millipore monoclonal
IgG
Mouse
(liquid, PBS, glycerol, HRP conj.)
ELISA, FA, WB Human immunogen = synthetic peptide corresp. to the human myelin basic protein sequence phosphor. at Thr98 and coupled to tuberculin reacts with myelin basic protein phosphor. at Thr98, 33kDa; some preparations of MBP contain basal levels of phosphorylated MBP, which are detected -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, TrisS, saline, azide, protein-G purified)
FA, WB Various vertebrate immunogen = peptide containing the seqeunce PRpTPP which corresp. to the human myelin basic protein sequence phosphor. at Thr98 reacts with in-vitro phosphorylated myelin basic protein, ~18-23 kDa -
Abcam monoclonal
IgG1
Mouse
(liquid, ascites, azide)
ELISA, IH, WB Dog, Cow, Sheep, Pig, Human, Mouse, Rat immunogen = purified human brain CNPase reacts specifically with myelin CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, protein A purified)
IC, IF, IH, WB Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat immunogen = purified human CNPase CNPase -
Abcam monoclonal
IgG1
Mouse
(liquid, ascites, azide)
DB, ELISA, IC, IF, IH, IP, WB Dog, Cow, Sheep, Pig, Human, Mouse, Rat immunogen = purified full length native human protein reacts specifically with CNPase; localizes both CNP1 (46kD) and CNP2 (48kD) bands -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
WB Rabbit, Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH CNPase -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, azide)
IC, IF, WB Human, Mouse immunogen = synthetic peptide conj. to KLH CNPase -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
WB Human immunogen = synthetic peptide derived from within residues 200 - 300 of human CNPase, conj. to KLH CNPase, both isoforms (46 and 48 kDa) -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted)
IH Cat, Dog, Goat, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat immunogen = full length purified human CNPase CNPase -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, azide, protein L purified)
IC, IF Human, Mouse immunogen = peptide common to human (NP_149124, NCBI) and mouse (P16330, NCBI) CNPase CNPase -
Abcam monoclonal
IgG
Mouse
(liquid, PBS, azide, protein A purified)
WB Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 350 to the C-terminus of human CNPase, conj. to KLH CNPase -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa121-420 mapping at the C-terminus of mouse CNPase CNPase, both isoforms 1 (46kDa) and 2 (48 kDa) -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Mammal (various), Cow, Human, Mouse, Rat immunogen = epitope mapping near the N-terminus of human CNPase CNPase -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Mouse, Rat immunogen = epitope mapping within an internal region of mouse CNPase CNPase -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
ELISA, IF, IH, WB Bovine, Cat, Dog, Goat, Porcine, Rabbit, Sheep, Human, Monkey, Mouse, Rat immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) recognizes proteins of 46kD (CNP1) and 48kD (CNP2), identified as two forms of CNPase -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, affinity purified)
IC, IH, WB Bovine, Dog, Porcine, Rabbit, Sheep, Human, Mouse, Rat immunogen = purified human 2'-3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) recognizes human CNPase, 48 and 46kD polypeptides -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, azide, affinity purified)
ELISA, IC, IH, WB Bovine, Dog, Rabbit, Sheep, Human, Rat immunogen = purified human brain CNPase (2'-3'-cyclic nucleotide 3'-phosphodiesterase) whole protein recognizes CNPase, 48 and 46kD polypeptides -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Mammal (various), Cow, Human, Mouse, Rat immunogen = epitope mapping near the N-terminus of human CNPase CNPase -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, no preservative, ammonium sulfate ppt.)
FC, IH, WB Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat immunogen = native protein CNPase; clearly differentiates oligodendrocytes and Schwann cells from meurons, astrocytes etc. -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic protein CNPase -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
IC, IH Mouse immunogen = synthetic protein conj. KLH; seq. of the synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) CNPase -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic protein CNPase -
Cell Signaling polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol)
WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to human CNPase endogenous levels of total CNPase -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, PBS, azide, protein A purified)
IC, IH, WB Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat immunogen = purified human brain CNPase CNPase, 48 (CNP2) and 46 kD (CNP1); clearly differentiates oligodendrocytes and Schwann cells from neurons, astrocytes, etc. -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, PBS, no preservative, protein A purified)
IC, IH, WB Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat immunogen = purified human brain CNPase CNPase, 48 (CNP2) and 46 kD (CNP1), identified as two isoforms -
Chemicon and Upstate are Millipore polyclonal
IgY
Chicken
(liquid, PBS, azide, affinity purified)
IC, IH Human, Mouse immunogen = synthetic peptide from human/mouse CNPase CNPase -
Novus Biologicals monoclonal
IgG1
Mouse
(liquid, ascites, PBS, azide, affinity purified)
DB, ELISA, IC, IH, IP, WB Dog, Rabbit, Cow, Sheep, Pig, Human, Mouse, Rat immunogen = full length native protein (purified) (human) CNPase localizes both CNP1 (46kD) and CNP2 (48kD) -
Novus Biologicals polyclonal
Chicken
(liquid, serum, PBS, azide, affinity purified)
IC, IH Mouse immunogen = synthetic peptide was shared between the human (NP_149124, NCBI) and mouse (P16330, NCBI) sequences, conj. KLH CNPase -
Novus Biologicals polyclonal
Rabbit
(liquid, serum, PBS, azide, affinity purified)
IH, WB Rabbit, Sheep, Human, Mouse, Rat immunogen = endogenous rabbit 2,3-cyclic nucleotide-3-phospho-diesterase CNPase -
Novus Biologicals polyclonal
Rabbit
(liquid antisera, PBS, azide, affinity purified)
WB Human immunogen = residues 68-84 of the 46-48 (doublet) kDa human CNPase protein (epitope: RVIVDKYRD GTKMVSAD) detects 2039-cyclic nucleotide phosphodiesterase -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, protein-G purified, phosphate buffer, saline, BSA, no preservatives)
WB Human, Mouse, Rat immunogen = peptide corresp. to the human myelin basic protein sequence containing a.a. Thr98 recognizes MBP; 18-20 kDa by WB -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, supernatant, Tris/HCL, fetal calf serum, azide)
ELISA, IH Bovine, Rabbit, Sheep, Human, Guinea Pig, Rat immunogen = bovine myelin basic protein (epitope in the region of a.a. 119-131) recognizes MBP -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, culture supernatant, azide)
ELISA, IH Various species, Human immunogen = human myelin basic protein (epitope in between a.a. 67-74: YGSLPQ) recognizes MBP -
Chemicon and Upstate are Millipore monoclonal
IgG2b
Mouse
(liquid, supernatant, azide)
IH Various vertebrate, Human immunogen = human myelin basic protein (recognizes an epitope of MBP between 84-89, a.a. DENPVV) recognizes MBP -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, protein-G purified, phosphate buffer, saline, azide, glycerol)
IH, WB Mouse, Rat immunogen = HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222 NG2 -
Abcam monoclonal
IgG2a
Rat
(liquid, tissue culture supernatant, azide)
ELISA, IH, RIA, WB Rabbit, Cow, Sheep, Human, Guinea Pig, Mouse, Rat immunogen = full length bovine myelin basic protein (epitope in the region of a.a. 82-87) reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP -
Chemicon and Upstate are Millipore polyclonal
Chicken
(liquid, affinity purified, PBS, azide)
IC, IH Mouse immunogen = synthetic peptide corresp. to human/mouse PLP protein recognizes PLP [Proteolipid Protein] -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(liquid, protein-A purified, PBS, azide)
IH, WB Mammal (various) immunogen = myelin proteolipid protein, synthetic peptide (CGRGTKF) corresp. to the C-terminal peptide recognizes myelin PLP; cross reactivity to DM20 -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, affinity purified, PBS, azide)
WB Rat immunogen = Synthetic Olig-2 peptide from rat recognizes Olig-2, a band at ~32 kDa in rat brain extract -
Novus Biologicals polyclonal
Rabbit
(liquid, whole antisera, azide)
IH Human, Rat immunogen = human myelin basic protein isolated from normal human brain reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord; also stains peripheral nerves -
Novus Biologicals polyclonal
Rabbit
(liquid, affinity purified antisera, PBS, azide)
FC, IH, WB Human, Mouse, Rat epitope at residues 49-62 of MBP, (DRGAPKR GSGKDSH) detects primarily the 22 kDa isoform of myelin basic protein -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, purified ascites, PBS, no preservatives)
FC, IH, IP, WB Human immunogen = tissue / cell preparation (Human - a 375P cell crude extract) detects MCSP; cell surface marker specific for epidermal stem cells -
Chemicon and Upstate are Millipore monoclonal
IgG2a
Mouse
(liquid, protein-A purified, PBS, saline, azide)
FC, IF, IP Human immunogen = human M14 melanoma cell extract depleted of fibronectin and bound by lens culinaris lectin-Sepharose™ recognizes mature chondroitin sulfate proteoglycan core glycoprotein of 250 kDa as well as precursor polypeptides of 210, 220 and 240 kDa -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, PBS, azide, protein-A affinity purified)
ELISA, IC, IH, IP, WB Human, Monkey, Mouse, Rat immunogen = immunoaffinity purified NG2 Chondroitin Sulfate Proteoglycan from rat identifies both the intact proteoglycan and the core protein by Western blot and ELISA -
R&D Systems monoclonal
IgM
Mouse
(filtered solution in PBS, trehalose, lyophilized)
FC, IH Chicken, Human, Mouse, Rat immunogen = white matter of corpus callosum from bovine brain recognizes Oligodendrocyte cell surface marker O1 -
Chemicon and Upstate are Millipore polyclonal
Ig
Rabbit
(liquid, purified Ig, Tris, glycine)
IC, IH, IP, WB Human, Mouse, Rat immunogen = recombinant mouse Olig-2 recognizes Oligodendrocyte marker Olig-2; recognizes the ~32 kDa Olig-2 protein by Western blot -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
IH Human, Rat immunogen = myelin basic protein isolated from normal human brain reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves -
Chemicon and Upstate are Millipore monoclonal
IgG3
Mouse
(liquid, purified, Potassium Phosphate, saline, azide)
ELISA, IC, IH, WB Bovine, Rabbit, Chicken, Human, Mouse, Rat immunogen = Synaptic plasma membranes from bovine hippocampus recognizes Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cellsGalC is a very useful, specific marker for oligodendroglial lineage -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, protein-A purified, phosphate buffer, saline, azide)
IC, IH, WB Bovine, Dog, Rabbit, Sheep, Pig, Human, Mouse, Rat immunogen = purified human brain CNPase recognizes CNPase, 48 and 46 kD polypeptides by SDS-PAGE; differentiates clearly oligodendrocytes and Schwann cells from neurons, astrocytes, etc -
AnaSpec polyclonal
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
IH Human immunogen = a Myelin basic protein isolated from a human brain reacts with Myelin Basic Protein -
AnaSpec polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
WB Pig, Human, Mouse, Rat immunogen = a synthetic peptide corresponding to the N-terminus of human myelin basic protein reacts with MBP-11 -
AnaSpec polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
WB Human, Mouse, Rat immunogen = a synthetic peptide corresponding to the amino acid sequence 139-151 of mouse proteolipid protein recognizes PLP -
AnaSpec polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
ELISA, WB Human, Mouse, Rat immunogen = a synthetic peptide corresponding to the amino acid sequence 178-191 of mouse proteolipid protein recognizes PLP -
StemCell Technologies monoclonal
IgM
Mouse
(liquid, purified Ig, phosphate buffer, azide)
IF Mouse immunogen = homogenate of white matter of corpus callosum from bovine brain O4 can be used to detect oligo dendrocytes in as early as day 3 cellcultures of brains from mouse embryos -
EMD Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, azide, undiluted ascites)
ELISA, IC, IH, WB Bovine, Dog, Porcine, Sheep, Human, Mouse, Rat immunogen = purified, human myelin CNPase recognizes the ~46 and ~48 kDa subunits of the 94 kDa myelin CNPase protein -