Akt1
Anaspec
polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = 16 aa peptide from near the N-terminus of human Akt1 (Genebank AAH00479)
AKT1 (48kDa)
-
Akt1 paired Thr308
Anaspec
polyclonal
Rabbit
(liquid PBS, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide surrounding Threonine308 from human/rat/mouse AKT1
AKT1
-
Akt1 phos Thr308
Anaspec
polyclonal
Rabbit
(liquid PBS, azide, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide corresponding to human AKT1 phosphorylated at Threonine308
detects AKT1 Phosphorylated at Threonine 308 only
-
Akt1 phos Ser473
Anaspec
polyclonal
Rabbit
(liquid PBS, Proclin 300, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide corresponding to C-terminus of human AKT1 containing phosphorylated site of Serine473
detects AKT1 phos Ser473. Does not react to other phosphorylated sites or non-phosphorylated AKT1
-
Akt2 C-term
Anaspec
polyclonal
Rabbit
(liquid PBS, azide, affinity purified)
-
Human
Immunogen = synthetic peptide corresponding to the C-terminus of human AKT2/PBK β (differs from corresponding mouse and rat sequence by a single amino acid)
AKT2
-
Akt1 phos Th308
Anaspec
polyclonal
Rabbit
(liquid PBS, azide, affinity purified)
-
Human, Mouse
Immunogen = synthetic phosphopeptide corresponding to human AKT1 at the phosphorylation site of threonine 308
detects AKT
-
Akt1 phos Ser473
Anaspec
polyclonal
Rabbit
(liquid immunoaffinity purified IgG, 50 μg in 250 μl of 1X PBS containing 0.05% sodium azide)
-
Human, Mouse
Immunogen = synthetic phosphopeptide derived from the region of human AKT that contains serine 473 (PQFpSYSA)
detects AKT1 phos Ser473
-
Akt3
Anaspec
polyclonal
IgG
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified)
-
Zebra fish
Immunogen = synthetic peptide derived from the intermediate region of zebrafish Akt3 (Genebank XP_00192252)
Akt3
-
Akt3 C-term
Anaspec
polyclonal
IgG
Rabbit
(liquid, MOPS, BSA, glycerol, affinity purified)
-
Zebra fish
Immunogen = synthetic peptide derived from the C-terminus of zebrafish Akt3 (Genebank XP_00192252)
Akt3
-
Akt3 N-term
Anaspec
polyclonal
Rabbit
(liquid PBS, azide, affinity purified)
-
Human, Mouse
Immunogen = synthetic peptide corresponding to the N-terminus of AKT3/PBK γ
detects Akt3
-
Akt, immobilized (1G1)
Cell Signaling
monoclonal
IgG2a
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of human Akt
binds preferentially to Akt phosphorylated at Ser473; also binds to some non-phospho-Akt
-
Akt phos Thr308 duet
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb #2965 and Akt (pan) (C67E7) Rabbit mAb #4691)
-
Human, Monkey, Hamster, Mouse, Rat, Drosophila
-
assess protein activation status
-
Akt PhosphoPlus® Activation Kit
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Thr308) (C31E5E) Rabbit mAb # 2965, Phospho-Akt (Ser473) (D9E) XP™ Rabbit mAb # 4060 and Akt (pan) (C67E7) Rabbit mAb # 4691)
-
Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Xenopus, Drosophila
immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues surrounding either Ser473, Thr308 or from the C-terminal sequence of mouse Akt
detect endogenous levels of Akt only when phosphorylated at either Ser473 or Thr308; Also detects endogenous levels of total Akt1/2/3 independent of their phosphorylation state; no cross-reaction with related kinases.
-
Akt phos Ser473 (587F11)
Cell Signaling
monoclonal
IgG2b
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
reacts with endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Bovine, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of humanAkt (KLH-coupled)
reacts with endogenous Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
reacts with endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473
Cell Signaling
kit
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Chicken, Human, Hamster, Mouse, Rat
Akt pS473 (#9271) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt.Akt (#9272) = synthetic peptide derived from the C-terminal seq of mouse Akt.
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.Akt detects total Akt1, Akt2 and Akt3.
-
Akt phos Ser473
Cell Signaling
kit
Rabbit
(ready-to-use, Protein A and affinity purified)
-
Human
AktpSer473 (#9277) = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects Akt1 when phosphorylated at Ser473 and Akt2 and Akt3 when phosphorylated at equivalent sites.
-
Akt phospho Pathway Sampler Kit
Cell Signaling
kit
Rabbit
(multi antibody sampler kit; HEPES, saline, BSA, glycerol, azide)
-
Chicken, Human, Monkey, Mouse, Rat
various antibodies for phospho.Akt pathway: phospho Akt,pan Akt,phospho c-Raf,phospho GSK-3β,phospho PTEN,phospho PDK1
-
Akt phos Thr308 (244F9)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt.
reacts with Akt phosphor. at Thr308 only
-
Akt phos Thr308 (244F9)
Cell Signaling
monoclonal
IgG
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Human
Immunogen = synthetic phospho-peptide (KLH-coupled) corresp. to residues aroundThr308 of mouse Akt
reacts with Akt phosphorylated at Thr308 only
-
Akt phos Thr308 (L32A4)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
reacts with endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phopho-peptide corresp. to residues around Thr308 of mouse Akt
reacts with Akt phos Thr308 only. Does not detect Akt phosphorylated at other sites or related kinases
-
Akt phos Thr308
Cell Signaling
kit
Unknown Host
(capture ab = Akt rabbit #9272; detection ab = monoclonal Akt pThr308 #5103 PathScan®)
-
Human, Mouse
-
detects Akt when phosphorylated at Thr308
-
Akt phos Thr405
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phopho-peptide corresp. to residues around Thr451 of Akt2
reacts with endogenous levels of Akt1 only when phosphorylated at Thr450; also recognizes Akt2 and Akt3 when phosphorylated at the corresponding residues; not Akt phosphorylated at other sites
-
Akt phos Tyr326
Cell Signaling
polyclonal
Rabbit
(HEPES, NaCl, BSA, glycerol, azide, protein A/affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues surrounding Tyr326 of Akt (KLH coupled)
reacts with Akt only when phosphorylated at Tyr326; no cross-reaction with Akt phosphorylated at other sites
-
Akt Signal Stain®
Cell Signaling
kit
Rabbit
(includes: Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) Rabbit mAb #4858 and Akt (pan) (C67E7) Rabbit mAb #4691PTEN (D4.3) Rabbit mAb #9188)
-
Human, Mouse
immunogen = synthetic phospho-peptides corresp. to residues surrounding the phosphorylation site(s) of interest, and synthetic peptides derived from the C-terminal seq. of mouse Akt or from the C-terminal seq. of human PTEN
paraffin-embedded tissues or cells with antibodies directed against proteins involved in Akt signaling
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488)
-
Human
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (193H12)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647)
-
Human
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (587F11)
Cell Signaling
monoclonal
IgG2b
Mouse
(liquid, HEPES, NaCl, BSA, glycerol, azide, biotinylated)
-
Human, Hamster, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
detects endogenous Akt only when phosphorylated at Ser473; no detection of Akt phosphorylated at other sites or related kinases such as PKC and p70 S6 kinase
-
Akt phos Ser473 (736E11)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of mouse Akt (KLH-coupled)
detects Akt only when phosphorylated at Ser473 and Akt2 and Akt3 only when phosphorylated at equivalent sites
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®488)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, conj. AlexaFluor®647)
-
Human, Mouse, Rat
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects Akt only when phosphorylated at Ser473
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Bovine, Dog, Chicken, Zebra fish, Human, Hamster, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Ser473
-
Akt phos Thr308 (244F9H2)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
reacts with endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®488)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
Akt phos Thr308 (C31E5E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, PBS, BSA, azide; conj. AlexaFluor®647)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues aroundThr308 of mouse Akt (KLH-coupled)
detects endogenous levels of Akt only when phosphorylated at Thr308
-
AKT/PKB (B052M)
Biodesign Meridian LifeScience
monoclonal
IgG1
Mouse
(lyophilized, supernatant, PBS, PEG, sucrose, azide, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminus (aa 466-480) coupled to KLH
AKT/PKB
-
AKT/PKB
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, serum, KPS, azide)
-
Chicken, Human, Mouse, Rat
Immunogen = synthetic peptide (C R P H F P Q F S Y S A S G T A) corresp. to the C-terminus (aa 460-480) of human, rat, mouse and chicken AKT proteins coupled to KLH
AKT
-
AKT/PKB
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non-phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C)
detects total Akt protein (aa 306-310)
-
AKT/PKB
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic non-phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S)
detects total Akt protein (aa 471-475)
-
AKT/PKB phos Ser473
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human
Immunogen = synthetic phosphopeptide derived from human Akt around the phosphorylation site of serine 473 (Q-F-Sp-Y-S)
detects Akt only when phosphorylated at Ser473
-
AKT/PKB phos Thr308
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
-
Human, Mouse, Rat
Immunogen = synthetic phosphopeptide derived from human Akt around the phosphorylation site of threonine 308 (M-K-Tp-F-C)
detects Akt only when phosphorylated at Thr308
-
AKT/PKB pS473
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human
Immunogen = synthetic peptide corresponding to the C-terminus aa 460-480 of human, mouse, rat, chicken AKT proteins coupled to KLH
AKT phosphorylated Ser473. Minimal reactivity against non-phosphorylated AKT
-
AKT/PKB pT308
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid, PBS, azide, affinity purified)
-
Human
Immunogen = synthetic peptide corresponding to aa 302-311 of human AKT protein coupled to KLH
AKT phosphorylated T308. Minimal reactivity against non-phosphorylated AKT
-
AKT2/PKBβ
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid,affinity purified, PBS, azide)
-
Human
Immunogen = synthetic peptide corresponding to aa 455-468 of human AKT2 protein coupled to KLH
AKT2 (phosphorylated and unphosphorylated)
-
AKT2/PKBβ
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid,affinity purified, PBS, azide)
-
Human, Mouse, Rat
Immunogen = synthetic non-phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S)
detects total Akt2 protein (amino acids 472-476)
-
AKT2/PKBβ phos Ser474
Biodesign Meridian LifeScience
polyclonal
Rabbit
(liquid,affinity purified, PBS, azide)
-
-
Immunogen = synthetic phosphopeptide derived from human Akt2 around the phosphorylation site of serine 474 (Q-F-Sp-Y-S)
detects AKT2 only when phosphorylated at serine 474
-
Akt phos Ser473
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, Protein A and affinity purified)
-
Bovine, Dog, Pig, Human, Hamster, Mouse, Rat, Drosophila
Immunogen = synthetic phospho-peptide corresp. to residues around Ser473 of mouse Akt
reacts with Akt when phosphorylated at Ser473; also reacts with Akt2 and Akt3 when phosphorylated at corresponding residues. Does not recognize Akt phosphorylated at other sites, nor does it recognize phosphorylated forms of related kinases such as PKC or p70 S6 kinase
-
Akt phos Ser473 (D9E)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Bovine, Zebra fish, Human, Monkey, Hamster, Mouse, Rat, Drosophila
immunogen = synthetic phospho-peptide corresp. to residues surrounding Ser473 of humanAkt
reacts with Akt only when phosphorylated at Ser473
-
AKT1 (2147)
AbD Serotec
monoclonal
Fab bivalent
Mouse
(purified)
-
Human
Immunogen = human Akt1 (val106-Ala480, MW 77.8kDa) fusion protein; contains the complete kinase domain (aa 150 to 408) but not the N-terminal PH domain
-
-
Akt1/2/3 phos Tyr315/316/312
Biotrend
polyclonal
IgG
Rabbit
(liquid, IgG fraction, affinity purified)
-
Human, Mouse, Rat
-
-
-
Akt
Stressgen Enzo Life Sciences
polyclonal
Rabbit
(liquid, PBS, glycerol, azide, protein A purified)
-
Human, Mouse, Rat
Immunogen = synthetic peptide derived from the sequence of human Akt aa 88-100
recognizes Akt/PKB (~59kDa); detects both phosphorylated and unphosphorylated PKB
-
Akt phos Thr308
Stressgen Enzo Life Sciences
polyclonal
Ig
Rabbit
(liquid, Dulbecco's PBS, glycerol, BSA, azide, affinity purified)
-
Human, Mouse
Immunogen = phospho-peptide sequence conserved between mouse and rat Akt1 and Akt2
Akt1 phosphorylated at Thr308. No reactivity with non-phosphorylated Akt/PKB
-
Akt2
Stressgen Enzo Life Sciences
polyclonal
Ig
Rabbit
(liquid, tris-acetate, azide, affinity purified)
-
Bovine, Dog, Rabbit, Chicken, Sheep, Pig, Human, Monkey, Guinea Pig, Hamster, Mouse, Rat, Xenopus
Immunogen = synthetic peptide (A S I R E), derived from seq. near the C-terminus of human Akt2 aa 477-481, conjugated to KLH
Akt2/PKBβ(~65kDa). Not PKBα (Akt1) or PKBγ (Akt3)
-
Akt phos Ser473
Stressgen Enzo Life Sciences
polyclonal
Ig
Rabbit
(liquid, affinity purified)
-
Bovine, Chicken, Human, Mouse, Rat
Immunogen = synthetic phospho-peptide derived from the C-terminus of Akt
Akt1, Akt2, Akt 3 phosphorylated at Ser473. No reactivity with non-phosphorylated Akt/PKB
-
Akt1/2
Stressgen Enzo Life Sciences
polyclonal
Rabbit
(liquid, PBS, azide, glycerol, protein A affinity purified)
-
Human, Mouse, Rat
immunogen = synthetic peptide derived from the C-terminus of human Akt
recognizes Akt1/2 (~56kDa)
-
Akt1/2 phos Ser473
Stressgen Enzo Life Sciences
kit
(colorimetric kit: microtiter plate, conjugate, antibody, assay buffer 32, wash buffer concentrate, standard, TMB substrate, cell lysis buffer 5, stop solution 2)
-
Human, Mouse, Rat
-
detects Akt1/2 phospho-Ser473; recognizes Akt3 with low affinity
-
Akt
Stressgen Enzo Life Sciences
kit
(non-radioactive Akt kinase activity assay: antibody, microtiter plate, conjugate, antibody dilution buffer, wash buffer concentrate, TMB substrate, stop solution 2)
-
Non-specific
-
measurement of Akt in partially purified, purified, or crude enzyme preparations from any species
-
Akt1 (5G12)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, PEG, sucrose)
-
Dog, Human, Rat
Immunogen = synthetic peptide corresp. to human Akt1 (Protein Kinase Bα; PKBα)
Akt1/PKBα
-
Akt3 (3B12F3F10)
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(liquid, ascites)
-
Human
immunogen = Ni-NTA purified truncated recombinant Akt3 expressed in E. coli strain BL21 (DE3)
Akt3
-
Akt3 (3B12F3F10)
LifeSpan Biosciences, Inc.
monoclonal
Mouse
(liquid, ascites)
-
Human
immunogen = Ni-NTA purified truncated recombinant Akt3 expressed in E. coli strain BL21 (DE3)
Akt3
-
Akt2 (8B7)
Alexis Enzo Life Sciences
monoclonal
IgG2a
Mouse
(lyophilized, PBS, glycerol, azide, PEG, sucrose, purified)
-
Human
Immunogen = synthetic peptide corresp. to human Akt2
Akt2
-
Akt, phos Ser473 (11E6)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(lyophilized supernatant, azide, PEG, sucrose, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminal (PQFpSYSA) of human Akt (protein kinase B; PKB) phosphorylated at Ser473
Ser473-phosphorylated Akt; no cross-reaction with dephosphorylated Akt or unrelated serine-phosphorylated proteins.
-
Akt3 (66C1247.1)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(liquid PBS, BSA, azide, Protein G purified)
-
Human
Immunogen = synthetic peptide corresp. to aa 119-136 of human Akt3.
detects Akt3, ~50 kDa by WB
-
Akt (5C10)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(lyophilized supernatant, azide, PEG, sucrose, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminal human Akt
recognizes a c-terminal epitope (aa 466-480) of Akt/PKB (phosphorylated and non-phosphorylated)
-
Akt
Alexis Enzo Life Sciences
polyclonal
Rabbit
(liquid PBS, BSA, azide, affinity purified)
-
Mouse
Immunogen = synthetic phospho-peptide corresp. to aa 302-314 of mouse Akt (PKB)
Akt/PBK (phosphorylated and non-phosphorylated)
-
Akt
Alexis Enzo Life Sciences
polyclonal
Rabbit
(liquid, neat serum, azide)
-
Mouse
Immunogen = recombinant mouse Akt
Recognizes mouse Akt; no cross with other species
-
Akt, dephos Ser473 (11A11)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(lyophilized, PBS, azide, PEG, sucrose, purified)
-
Dog, Human, Mouse, Rat
Immunogen = synthetic peptide corresp. to C-terminal (QFpSYS A) of human Akt (protein Kinase B; PKB) dephosphorylated at Ser472/473/474
Akt dephosphorylated at Ser473; no cross-reaction with akt phosphorylated at Ser473
-
Akt (19G7)
Alexis Enzo Life Sciences
monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide, protein A purified)
-
Dog, Human, Hamster, Mouse, Rat
Immunogen = recombinant mouse Akt (protein kinase B; PKB)
Detects Akt1 (PKB-α) and Akt2 (PKB-β) strong than Akt3 (PKB-γ). Detects also phosphorylated forms of Akt
-
Akt1 (6D109)
LifeSpan Biosciences, Inc.
monoclonal
IgG1 , κ
Mouse
(lyophilized, purified)
-
Dog, Human, Mouse, Rat
immunogen = synthetic peptide sequence (aa466-480) located at the C-terminus of Akt (KLH coupled)
Akt1
-
Akt1/2 (N-19)
Santa Cruz
polyclonal
IgG
Goat
(liquid, PBS, gelatin, azide)
-
Human, Mouse, Rat, Xenopus
Immunogen = peptide mapping at the N-terminus of human Akt1
Akt1 and Akt2
-
p70 S6 Kinase phos Thr389 (108D2)
Cell Signaling
monoclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol)
-
Human, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401
-
p70 S6 Kinase phos Thr389
Cell Signaling
polyclonal
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, azide)
-
Human, Monkey, Mouse, Rat
Immunogen = synthetic phospho-peptide corresp. to residues around Thr389 of human p70 S6 kinase
detects endogenous levels of p70 S6 kinase only when phosphorylated at threonine 389; also detects p85 S6 kinase when phosphorylated at the analogous site (Thr412), and possibly S6KII phosphorylated at Thr401
-