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Antibodies

136 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
14-3-3s From manufacturers (100)
Biolegend polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB Human immunogen = full-length human 14-3-3 zeta protein 28 kDa 14-3-3ζ -
Biolegend polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB Human, Mouse immunogen = NIH-recombinant (partial), N-terminal 14-3-3 protein 30 kDa 14-3-3ζ/δ -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, serum)
ELISA, IH, WB Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat immunogen = synthetic corresp. to C-terminal sequence of sheep 14-3-3ε 14-3-3ε (C-terminal, 30kDa) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, serum)
ELISA, IH, WB Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3ε 14-3-3ε (N-terminal, 30kDa) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Mouse
(liquid, PBS, unpurified serum)
ELISA, WB Human immunogen = human YWHAE (aa1-256) with GST Tag 14-3-3 protein epsilon (YWHAE) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to a region of human 14-3-3η (YWHAH) 14-3-3 protein eta(YWHAH) -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, serum)
IH, WB Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 eta 14-3-3 protein eta(YWHAH) -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
IF, IH, IP, WB Human immunogen = recombinant human 14-3-3 sigma protein (26-30kD) 14-3-3 protein σ; no cross with other isoforms of 14-3-3 -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
IF, IH, IP, WB Human immunogen = recombinant human 14-3-3 sigma protein (26-30kD) 14-3-3 protein σ; no cross with other isoforms of 14-3-3 -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
IF, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to aa1-248 14-3-3 protein σ; (Stratifin) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, BSA, azide)
IH, IP, WB Human, Rat immunogen = recombinant human 14-3-3 sigma protein (26-30kD) 14-3-3 protein σ -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, azide, affinity purified)
IP, WB Human, Mouse, Rat immunogen = GST fusion protein corresponding to full-length human 14-3-3 sigma 14-3-3 protein σ; no cross-reaction with other 14-3-3 isoforms -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(liquid, serum)
WB Mammal (various), Bovine, Rabbit, Chicken, Sheep, Human, Mouse, Rat immunogen = synthetic peptide corresp. to acetylated N-terminal sequence of sheep 14-3-3 sigma 14-3-3 protein σ (SFN) -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, purified)
ELISA, IP, WB Bovine, Human, Mouse, Rat immunogen = recombinant 14-3-3 theta/tau (a single protein which is known by the alternative names 14-3-3θ and 14-3-3τ) 14-3-3 proteinθ/τ; no cross-reaction with 14-3-3ζ -
LifeSpan Biosciences, Inc. monoclonal
IgG1
Mouse
ELISA, WB Bovine, Human, Mouse, Rat - - -
LifeSpan Biosciences, Inc. polyclonal
IgG
Rabbit
(lyophilized, affinity purified)
IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresp. to a region of human 14-3-3τ (YWHAQ) 14-3-3τ (YWHAQ) -
LifeSpan Biosciences, Inc. monoclonal
IgG1, κ
Mouse
(liquid, PBS, glycerol, no preservative, affinity purified)
IP, WB Human immunogen = recombinant protein of human 14-3-3 gamma (26-33kD) recognizes beta, gamma, sigma and zeta isoforms of 14-3-3 -
Novus Biologicals monoclonal
IgG1
Mouse
(liquid, ascites)
WB Human immunogen = an N-terminal fragment of human 14-3-3 gamma specific for human non-modified N-terminus 14-3-3 gamma; naturally occurring form of 14-3-3 gamma in which the N-terminus Met is removed and the Val is acetylated isnot recognized by this antibody -
Novus Biologicals monoclonal
IgG2a
Mouse
(liquid, ascites)
WB Human immunogen = an N-terminal fragment of human 14-3-3 gamma where the N-terminal Val was acetylated specific for human 14-3-3 gamma; where the N-terminal Met is removed, resulting in an acetylated N-terminal Val. Unprocessed (non-modified) 14-3-3 gamma is not recognized by this antibody -
US Biological monoclonal
IgG1
Mouse
(liquid, ascites, HEPES, NaCl, BSA, glycerol, azide)
ELISA, IP, WB - immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled) wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences -
US Biological polyclonal
IgG
Rabbit
(liquid, HEPES, NaCl, BSA, glycerol, affinity purified)
ELISA, IH, IP, WB - immunogen = synthetic phospho-(Ser) 14-3-3 binding motif peptides (KLH coupled) binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position; no cross-reaction with corresp. non-phosphorylated sequences or with other phospho-Thr/Ser/Tyr -containing motifs -
US Biological monoclonal
IgG2a, κ
Mouse
(liquid, PBS, azide, protein G affinity purified)
ELISA, WB Human immunogen = recombinant human 14-3-3 epsilon (1-255aa) purified from E. coli wide range of peptides containing this phosphorylated 14-3-3 binding motif; no cross-reaction with corresponding non-phosphorylated sequences -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purfied)
WB Bovine, Human, Mouse, Rat immunogen = recombinant protein from human YWHAE (aa1-255) 14-3-3 protein epsilon (YWHAE) -
LifeSpan Biosciences, Inc. polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
WB Human, Mouse, Rat immunogen = recombinant protein corresp. to aa1-248 from human SFN 14-3-3 protein σ; (Stratifin) -
LifeSpan Biosciences, Inc. monoclonal
IgG
Mouse
(liquid, PBS, BSA, glycerol, azide, affinity purified)
IF, IH, WB Dog, Rabbit, Human, Monkey, Mouse, Rat - recognizes a ~31 kDa protein, corresponding to the apparent molecular weight of the beta and zeta isoforms of 14-3-3 -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
IC, IF, IP, WB Human, Rat immunogen = recombinant full length human 14-3-3 gamma protein 14-3-3 protein (beta, gamma and zeta isoforms) -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, PBS, azide, BSA, thimerisol)
IP, WB Human, Mouse, Rat immunogen = a mixture of 14-3-3 fusion proteins (beta, eta, tau, and sigma) 14-3-3 isoforms -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide with the sequence D K S E L V Q K A K L A E Q A E R Y D, mapping to the N-terminal domain of human 14-3-3 14-3-3 isoforms (alpha, beta, zeta, and theta) -
Abcam monoclonal
IgG1
Mouse
(liquid, HEPES, NaCl, glycerol, BSA, no preservative, protein A purified)
ELISA, IP, WB - immunogen = synthetic phospho(Ser) 14-3-3 binding motif peptides (KLH coupled) binds peptides/ proteins containing phospho-Ser surrounded by Pro at the +2 position and Arg/Lys at the -3 position. No crossreactivity is observed with corresp. nonphosphorylated seq. -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, WB Cow, Human, Mouse, Rat, Xenopus, C. elegans immunogen = 20 aa synthetic peptide derived from the N-terminus of the human 14-3-3 beta/alpha protein broadly reactive with members of the 14-3-3 family of proteins (~29–32kDa) -
Abcam polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
WB Human, Rat immunogen = phosphopeptide corresp. to aa residues surrounding the phosphorylation site Ser58 of rat 14-3-3 protein specific for the ~29k 14-3-3 protein phosphorylated at Ser58 -
Abcam polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, affinity purified)
WB Human, Mouse, Rat, Xenopus immunogen = synthetic peptide corresp. to aa residues from the C-terminal region of human 14-3-3 protein, conj. to KLH 14-3-3 protein -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
WB Human, Mouse, Rat immunogen = full length E coli-derived fusion protein 14-3-3 protein -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
IP, WB Human immunogen = recombinant full length human 14-3-3 gamma protein 14-3-3 protein -
Abcam polyclonal
IgG
Sheep
(liquid, PBS, azide, protein G purified)
WB Human immunogen = full length native 14-3-3 protein purified from human brain recognises the lower molecular weight subunit (26 kDa) of 14-3-3 -
Abcam monoclonal
IgG2a
Mouse
(liquid, supernatant, no preservative)
WB Rat immunogen = synthetic peptide, corresp. to aa208-244 of rat 14-3-3 14-3-3 protein (33 kDa) -
Abcam monoclonal
IgG
Rabbit
(liquid, tris-glycine, NaCl, BSA, glycerol, azide)
FC, IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues in the C-terminal of human 14-3-3 alpha + beta specific for 14-3-3 alpha + beta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, sucrose, affinity purified)
IH, WB Human immunogen = synthetic peptide, corresp. to a region between aa71-120 of human 14-3-3η 14-3-3eta (28kDa) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
ELISA, IC, IF, IH, WB Human, Mouse, Rat immunogen = synthetic peptide derived from internal of human 14-3-3 gamma detects endogenous levels of total 14-3-3 gamma protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, IC, IF, IH, WB Human, Mouse immunogen = synthetic non-phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-TP-Q-G) detects endogenous levels of total 14-3-3 zeta + delta protein. -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
ELISA, IH, WB Human immunogen = synthetic peptide selected within aa 200~300 of human 14-3-3 beta, conj. KLH binds both transcript variants of 14-3-3 (alpha and beta) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
ELISA, IH, WB Human immunogen = synthetic peptide selected within aa 50~150 of human 14-3-3 beta (N-terminal), conj. KLH binds to both isoforms of 14-3-3 (alpha and beta) -
Abcam monoclonal
IgG
Rabbit
(liquid, Ig fract., tris-glycine, NaCl, BSA, glycerol, azide)
FC, IH, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues in the N-terminal of human 14-3-3 alpha + beta specific for 14-3-3 alpha + beta -
Abcam monoclonal
IgG
Rabbit
(liquid, PBS, PMSF, glycerol, no preservative, protein G purified)
IC, IH, WB Dog, Rabbit, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat immunogen = lysed transformed amnion cells (AMA) (human) specific for 14-3-3 alpha + beta -
Abcam polyclonal
IgG
Rabbit
(liquid, HEPES, BSA, NaCl, no preservative, glycerol, protein A purified)
ELISA, IH, IP, WB - immunogen = synthetic phospho (Ser) 14-3-3-binding-motif peptides binds peptides and proteins containing a motif composed of phospho-Ser with proline at the +2 position and arginine or lysine at the -3 position -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum)
ELISA, IH, WB Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide from within N-terminus of 14-3-3 epsilon 14-3-3epsilon; may not react with recombinant protein expressed in E. coli that is not N-acetylated -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IC, IF, WB Human, Mouse, Rat immunogen = synthetic peptide (human) from an internal region detects endogenous levels of total 14-3-3eta -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, no preservative, protein G purified)
IP, WB Human immunogen = recombinant full length human 14-3-3 gamma protein 14-3-3γ; no crossreactivity with other isoforms of 14-3-3 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, NaCl, glycerol, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthetic phosphopeptide from human 14-3-3-zeta, around the phosphorylation site of serine 58 (R-S-pS-W-R) endogenous levels of 14-3-3 zeta only when phosphorylated at serine 58 (28kDa) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, protein G purified)
ELISA, IH, WB Human immunogen = synthetic peptide selected within aa 200-300 of human 14-3-3 zeta (C-terminal) specific for 14-3-3 zeta + delta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, IH, WB Human, Mouse, Rat immunogen = synthesized non-phosphopeptide derived from human 14-3-3 zeta around the phosphorylation site of serine 58 (R-S-Sp-W-R) endogenous levels of total 14-3-3 zeta protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified)
IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal) 14-3-3beta -
Abcam monoclonal
IgG2b, κ
Mouse
(liquid, PBS, no preservative, glycerol, protein A purified)
ELISA, WB Human, Mouse, Rat immunogen = recombinant full length human protein 14-3-3beta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide from C-terminus of human 14-3-3 epsilon detects endogenous levels of total 14-3-3 epsilon protein -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum)
ELISA, IH, WB Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide from C-terminus of 14-3-3 epsilon 14-3-3epsilon -
Abcam polyclonal
IgY
Chicken
(liquid, PBS, no preservative, affinity purified)
WB Cow, Human, Mouse, Rat immunogen = full length protein human 14-3-3 epsilon 14-3-3epsilon -
Abcam monoclonal
IgG2a, κ
Mouse
(liquid, PBS, azide, protein G purified)
ELISA, WB Human immunogen = recombinant full length human protein purified from E. coli 14-3-3epsilon -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, no BSA, glycerol, no preservative protein G purified)
IC, IF, IH, IP, WB Human immunogen = recombinant full length human 14-3-3σ specific to 14-3-3 sigma; shows no cross-reaction with other isoforms of 14-3-3 -
Abcam polyclonal
IgG
Rabbit
(liquid, whole serum, azide)
IH Human immunogen = synthetic peptide: S N E E G S E E K G P E V, corresp. to internal seq. aa69-81 of human 14-3-3 sigma specific to 14-3-3 sigma -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
ELISA, WB Human, Mouse immunogen = synthetic peptide human 14-3-3 sigma detects endogenous levels of total 14-3-3 sigma protein -
Abcam polyclonal
IgG
Goat
(liquid, TBS, BSA, azide, affinity purified)
ELISA, WB Human immunogen = synthetic peptide: D L H T L S E D S Y K D S T from the internal region of human 14-3-3 sigma protein seq. (NP_006133.1) 14-3-3 sigma protein -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, glycerol, azide, protein G purified)
IC, IF, IH, IP, WB Human, Mouse immunogen = recombinant full length protein (human) recognizes human 14-3-3 sigma protein, 26-30kD; no crossreaction with other isoforms of 14-3-3 -
Abcam monoclonal
IgG1
Mouse
(liquid, Tris-glycine, NaCl, glycerol, azide, protein G purified)
IH, WB Human, Mouse, Rat immunogen = recombinant full length human 14-3-3σ recognizes 14-3-3 sigma and does not crossreact with other 14-3-3 isoforms. It has been specifically tested against 14-3-3 gamma, beta, zeta, and epsilon -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified, prediluted)
IH Human immunogen = recombinant human 14-3-3σ specific to 14-3-3 sigma -
Abcam monoclonal
IgG1
Mouse
(liquid, PBS, glycerol, azide, protein G purified)
IP, WB Cow, Human, Mouse, Rat immunogen = recombinant full length protein (human) recognises 14-3-3-theta/tau isoform; no reaction with the 14-3-3 zeta isoform -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, NaCl, affinity purified)
ELISA, IC, IF Human, Mouse immunogen = synthetic phosphopeptide derived from human 14-3-3 Tau around the phosphorylation site of serine 232 (S-D-Sp-A-G) detects 14-3-3 Tau only when phosphorylated at serine 232 -
Abcam polyclonal
IgG
Mouse
(liquid, whole serum, glycerol, no preservative)
WB Mouse immunogen = vector coding for a partial recombinant fusion protein, corresp. to aa51-151 of mouse 14-3-3 Tau detects 14-3-3 Tau -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, no preservative, glycerol, protein A purified)
WB Human, Mouse, Rat immunogen = recombinant full length protein (human) 14-3-3beta -
Abcam monoclonal
IgG1, κ
Mouse
(liquid, PBS, no preservative, no BSA, protein G purified)
IP, WB Human immunogen = recombinant full length human 14-3-3 gamma protein 14-3-3γ; no crossreactivity with other isoforms of 14-3-3 -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
ELISA, IH, WB Chicken, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide corresp. to acetylated N- terminal seq. of sheep 14-3-3 Tau recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, IC, IF Human, Mouse immunogen = synthetic non-phosphopeptide derived from human 14-3-3 Tau near phosphorylation site of serine 232 (S-D-Sp-A-G) recognises 14-3-3 tau; may not react with recombinant proteins that are not N-acetylated -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, NaCl, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide derived from internal sequence of human 14-3-3 Tau detects endogenous levels of total 14-3-3 Tau protein -
Abcam polyclonal
IgG
Goat
(liquid, TBS, BSA, azide, affinity purified)
ELISA, WB Human immunogen = Synthetic peptide: C-D D R K Q T I D N S Q, corresp. to internal seq. aa136-146 of human 14-3-3 Tau (NP_006817.1) detects endogenous levels of total 14-3-3 Tau protein -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, azide, glycerol, affinity purified)
ELISA, IH Human, Mouse immunogen = synthetic phosphopeptide derived from human 14-3-3 zeta + delta around the phosphorylation site of threonine 232 (S-D-Tp-Q-G) detects endogenous levels of 14-3-3 zeta + delta only when phosphorylated at threonine 232 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IH, WB Human, Mouse, Rat immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta specific for 14-3-3 zeta (30kDa) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, NaCl, glycerol, azide, affinity purified)
ELISA, WB Human, Mouse, Rat immunogen = synthetic peptide from an internal region of human 14-3-3 zeta specific for 14-3-3 zeta (30kDa) -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, affinity purified)
ELISA, WB Cow, Human, Mouse, Rat, Xenopus, C. elegans immunogen = synthetic peptide derived from the N-terminus of human 14-3-3 alpha/beta protein broadly reactive with the ~29-32 kDa members of the 14-3-3 family of proteins -
Abcam monoclonal
IgG
Mouse
(liquid, PBS, no preservative, glycerol, PMSF, protein G purified)
IC, WB Dog, Cow, Sheep, Pig, Human, Monkey, Mouse, Rat immunogen = tissue / cell preparation (lysed transformed human amnion cells) 14-3-3beta/zeta -
Abcam monoclonal
IgG2b, κ
Mouse
(liquid, PBS, azide, sucrose, protein A purified)
IP, WB Mouse, Rat immunogen = recombinant full length rat protein 14-3-3beta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, azide, BSA, affinity purified, prediluted)
IH Human, Mouse, Rat immunogen = synthetic peptide (unfortunately, the aa seq. is considered to be commercially sensitive) (human) (N-terminal) 14-3-3beta -
Abcam polyclonal
IgG2a, κ
Rabbit
(liquid, PBS, no preservative, protein G purified)
WB Human immunogen = recombinant fragment corresp. to aa71-170 of human 14-3-3 eta 14-3-3eta -
Abcam monoclonal
IgG2b, κ
Mouse
(liquid, PBS, no preservative, protein G purified)
WB Human immunogen = recombinant fragment corresp. to aa70-170 of human 14-3-3 gamma 14-3-3γ -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, no preservative)
ELISA, WB Chicken, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 gamma 14-3-3 gamma protein -
Abcam monoclonal
IgG, κ
Mouse
(liquid, PBS, BSA, azide, protein G purified)
IP, WB Human immunogen = recombinant full length human 14-3-3 gamma protein recognizes both zeta and gamma isoforms. This antibody shows no crossreactivity with other isoforms of 14-3-3 -
Abcam monoclonal
IgG1
Mouse
(liquid, glycerol, azide, PBS, conj. agarose)
IP, WB Cow, Human, Mouse, Rat immunogen = recombinant full length protein (human) reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta -
Abcam monoclonal
IgG1
Mouse
(liquid, glycerol, azide, PBS, conj. HRP)
IP, WB Cow, Human, Mouse, Rat immunogen = recombinant full length protein (human) reacts specifically with the Tau + Theta isoform and does not react with 14-3-3 zeta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, glycerol, azide, affinity purified)
WB Human, Mouse immunogen = recombinant frag., corresp. to N-terminal aa1-150 of 14-3-3 zeta specific for 14-3-3 zeta + delta -
Abcam polyclonal
IgG
Mouse
(liquid, whole serum, glycerol, no preservative)
WB Mouse immunogen = recombinant fusion protein corresp. to aa51-150 of mouse 14-3-3 zeta specific for 14-3-3 zeta (30kDa) -
Abcam monoclonal
IgG2a
Mouse
(liquid, ascites)
WB Sheep, Rat immunogen = tissue / cell preparation (sheep pineal gland) 14-3-3 zeta -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified, prediluted)
IH Human, Mouse, Rat immunogen = synthetic peptide: A G D D K K G I V D Q S Q Q A Y, corresp. to aa134 - 149 of human 14-3-3 zeta specific for 14-3-3 zeta -
Abcam polyclonal
IgG
Sheep
(liquid, BSA, azide, glycerol, TBS, affinity purified)
WB Cow, Sheep, Human, Mouse, Rat immunogen = synthetic phosphopeptide corresp. to aa surrounding phospho Serine185 recognizes 14-3-3 phospho Serine185. It is specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue -
Abcam polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
IH, WB Mammal (various) immunogen = Synthetic peptide: K M K G D Y Y R Y L A E, corresp. to aa124-135 of 14-3-3 eta + gamma + zeta recognizes eta, gamma and zeta isoforms at 27kDa -
Abcam polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
WB Various vertebrate immunogen = HPLC purified synthetic peptide (K M K G D Y Y R Y L A E) corresp. to the zeta, gamma and eta isoforms of 14-3-3 recognizes eta, gamma and zeta isoforms -
Abcam polyclonal
IgG
Rabbit
(liquid, whole serum, no preservative)
WB Rabbit, Chicken, Cow, Sheep, Human, Mouse, Rat immunogen = synthetic peptide from the N-terminal area of sheep 14-3-3 eta 14-3-3eta -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Arabidopsis thaliana immunogen = epitope mapping near the C-terminus of Arabidopsis thaliana 14-3-3 14-3-3 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Arabidopsis thaliana immunogen = epitope corresp. to aa 21-75 mapping within an internal region of Arabidopsis thaliana 14-3-3 14-3-3 and to a lesser extent 14-3-3-like proteins -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = raised against aa 1-246 representing full length human 14-3-3β 14-3-3 -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = raised against aa 1-246 representing full length human 14-3-3β 14-3-3 -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = raised against aa 1-246 representing full length human 14-3-3β 14-3-3 protein family -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, FC, IF, IH, IP, WB Human, Mouse, Rat immunogen = epitope mapping at the N-terminus of human 14-3-3β detects members of 14-3-3 protein family -