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Antibodies

60 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Organelles From manufacturers (60)
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, supernatant, PBS, no presrvative, protein A purified)
IH, IP Primate (various), Human - Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells -
Chemicon and Upstate are Millipore monoclonal
IgG1
Mouse
(liquid, supernatant, purified, concentrate, PBS, azide)
IF, IH, IP Human epitope on surface of intact mitochondria recognizes a 60kDa nonglycosylated protein component of mitochondria found in human cells -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(liquid, serum, purified, azide)
IC, WB Human, Monkey immunogen = Presenilin 1 peptide conjugated to KLH specific to mitochondria; recognizes a 62 kD protein of immunoblots of subcellular fractions enriched in mitochondrial fractions -
Molecular Probes Invitrogen monoclonal
IgG1
Mouse
(lyophilized)
ELISA, IF, IP, WB Bovine, Human, Mouse, Rat immunogen = OxPhos Complex I, isolated from bovine heart,bovine liver, and human heart recognizes Complex I (NADH-ubiquinoloxidoreductase), 39 kDa subunit. -
Biolegend monoclonal
IgG2b, κ
Rat
(liquid, affinity purified, PBS, azide)
FC, IF, IH, IP Mouse immunogen = mouse embryo 3T3 cells LAMP-1 -
Biolegend monoclonal
IgG2b, κ
Rat
(liquid, affinity purified, PBS, azide, conj. FITC)
FC, IF, IH Mouse immunogen = mouse embryo 3T3 cells LAMP-1 -
Biolegend monoclonal
IgG2b, κ
Rat
(liquid, affinity purified, PBS, azide, conj. Biotin)
FC, IF, IH Mouse immunogen = mouse embryo 3T3 cells LAMP-1 -
Biolegend monoclonal
IgG2b, κ
Rat
(liquid, affinity purified, PBS, azide, conj. Alexa Fluor® 488)
FC, IF, IH Mouse immunogen = mouse embryo 3T3 cells LAMP-1 -
Biolegend monoclonal
IgG2b, κ
Rat
(liquid, affinity purified, PBS, azide, conj. Alexa Fluor® 647)
FC, IF, IH Mouse immunogen = mouse embryo 3T3 cells LAMP-1 -
Biolegend monoclonal
IgG1, κ
Rat
(liquid, PBS, azide, affinity purified)
FC, IH, IP Mouse immunogen = membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells recognizes Mac-3 in macrophages, some myeloid cell lines -
DSHB U. Iowa monoclonal
IgG2a
Rat
(liquid, affinity purified, PBS, azide)
IB, IH, IP Mouse immunogen = Lamp-1 from NIH/3T3 mouse embryo fibroblast tissue culture cell membranes (epitope in N-terminus) 110 kDa Lamp-1; labels lysosome membranes -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, antisera, Tris, glycine, azide, affinity purified)
IF Human, Mouse, Rat immunogen = synthetic peptide: CKRSHAGYQT , corresp. to amino acids 407-417 of Human LAMP1 detects LAMP1 -
Novus Biologicals monoclonal
IgG
Rat
(liquid, ascites, PBS, glycerol, azide, protein-G purified)
IC, IP Human, Mouse, Rat immunogen = purified preparation of mouse liver lysosomal membranes detects LAMP2; will label the presumptive lysosomes and late endosomes in cells that have been permeabilized with saponin -
BD Pharmingen monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, stabilizer, azide, affinity purified, Alexa Fluor® 488)
IF Dog, Human, Mouse, Rat immunogen = rat C-terminal GM130 recombinant protein recognizes GM130, regardless of phosphoryl. status -
Abcam monoclonal
IgG2a
Mouse
(liquid, PBS, protein A purified)
EM, IC, IF, IH, WB Rat immunogen = recombinant full length rat protein recognizes GM130 -
Abcam polyclonal
IgG
Rabbit
(liquid, BSA, azide, glycerol, Tris-glycine, citric acid, EDTA, NaCl)
IC, IF, IH, WB Human immunogen = synthetic peptide (human) GM130 -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IC, IF, WB Drosophila immunogen = synthetic peptide derived from within residues 750 to the C-terminus of fruit fly (Drosophila melanogaster) GM130, conjugated to KLH GM130 -
Abcam monoclonal
IgG
Rabbit
(liquid, PBS, azide, glycerol, Tris-glycine saline, EDTA, citric acid)
IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues near the N-term of human GM130 GM130 -
Abcam polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, BBS, EDTA, affinity purified)
IC, IF Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 950 to the C-terminus of rat GM130 GM130 -
Abcam monoclonal
IgG
Rabbit
(liquid, supernatant, BSA, azide, glycerol, Tris-glycine saline)
FC, WB Human immunogen = synthetic peptide corresp. to residues near the N-term of human GM130 GM130 -
Abcam polyclonal
IgG
Rabbit
(liquid, BSA, PBS, azide, affinity purified)
WB Drosophila immunogen = synthetic peptide derived from within residues 1-100 of fruit fly (Drosophila melanogaster) GM130, conj. to KLH GM130 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping at the C-terminus of human GM130 GM130 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IH, IP, WB Human, Mouse, Rat immunogen = raised against aa191-255 mapping within an internal region of human GM130 GM130 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = raised against aa191-255 mapping within an internal region GM130 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa191-255 mapping within an internal region of human GM130 GM130 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Rat immunogen = recombinant rat GM130 GM130 -
Santa Cruz monoclonal
IgG2a
Mouse
(liquid, PBS, azide, gelatin)
IF, IH, IP, WB Rat immunogen = recombinant rat GM130 GM130 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Mouse, Rat immunogen = epitope mapping within an internal region of rat GM130 GM130 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Human immunogen = epitope mapping within an internal region of human GM130 GM130 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, IP, WB Mouse, Rat immunogen = epitope mapping near the N-terminus of rat GM130 GM130 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope corresp. to a short aa seq. containing phosphorylated Ser of human GM130 GM130 phosphorylated at Ser25 -
Novus Biologicals monoclonal
IgG
Rabbit
(liquid, ascites, azide, glycerol, Tris-glycine saline, EDTA, citric acid)
IC, IH, IP, WB Human, Mouse, Rat immunogen = synthetic peptide corresp. to residues near the N-term of human GM130 GM130 -
Novus Biologicals monoclonal
IgG2a
Mouse
(liquid, PBS, purified)
IH, WB Human, Mouse, Rat immunogen = recombinant full length protein (rat) GM130 -
AnaSpec polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
ELISA, IF, WB Human, Mouse, Rat immunogen = a 15 amino acid peptide from near the center of human LAMP-1 detects LAMP-1 -
AnaSpec polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, azide)
IH, WB Human, Mouse immunogen = a 17 amino acid peptide from near the center of human LAMP-2 detects LAMP-2 -
Cell Signaling polyclonal
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
IP, WB Human, Monkey immunogen = a synthetic peptide corresponding to the amino-terminal sequence of human GM130 detects endogenous levels of total GM130 protein -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol, azide)
WB Human, Monkey, Mouse immunogen = a synthetic peptide surrounding Ser140 of human LAMP1 detects endogenous levels of total LAMP1 protein -
DSHB U. Iowa monoclonal
IgG1
Mouse
(liquid, available as supernatant, concentrate, ascites)
IB Human immunogen = human Lamp-2 Lamp-2 -
Novus Biologicals polyclonal
IgG
Rabbit
(liquid, affinity purified, PBS, BSA, azide)
WB Human immunogen = synthetic peptide corresp. to residues G(a.a. 368)QVMESVRQ LQMERDK(a.a. 383) of human GM130 detects GM130; an approx. 135 kDa band -
Novus Biologicals polyclonal
IgG
Mouse
(liquid, antisera, no preservativeAlso available purified, H00002801-B01P)
ELISA, IF, IH, WB Human immunogen = GOLGA2 (1 a.a. ~ 345 a.a) full-length human protein detects GOLGA2/GM130; reactive against immunizing recombinant protein, transfected lysate and tissue lysate for WB -
Novus Biologicals monoclonal
IgG1
Mouse
(liquid, purified IgG, PBS, no preservative)
ELISA, WB Human immunogen = GOLGB1 (NP_004478, 3 a.a. ~ 92 a.a) partial recomb. protein with GST tag detects giantin; reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control -
Alexis Enzo Life Sciences monoclonal
IgG1
Mouse
(affinity purified, gel filtrated (FPLC), lyophilized)
ELISA, FC, IC, IH, IP Mammal (various), Avian (various), Human, Monkey immunogen = Giantin from Caco-2 cell Golgi membrane fraction Recognizes giantin -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(serum, affinity purified, PBS, azide)
ELISA, WB Rat immunogen = synthetic peptide derived from the cytosolic region of the rat Lamp-2 (lgp96) protein detects the ~96 kDa rat Lamp-2 (lgp96) -
BD Pharmingen monoclonal
IgG2a, κ
Rat
(liquid, affinity purified, aqueous, buffered, azide, FITC conj.)
FC, IC Mouse immunogen = glycoproteins purified from BALB/c mouse embryo 3T3 cell line detects LAMP-2 -
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 555 conj.)
IF Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 488 conj.)
IF Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, protein stabilizer, azide, Alexa Fluor® 647 conj.)
IF Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
DSHB U. Iowa monoclonal
IgG2a
Rat
(liquid, affinity purified, PBS, azide)
IB, IH, IP Mouse immunogen = Lysosomal membrane glycoprotein B (lgp-B), from mouse liver homogenate 110 kDa Lamp-2; labels presumptive lysosomes and late endosomes -
ABGENT polyclonal
Ig
Rabbit
(liquid, PBS, azide, Protein A affinity purified)
ELISA, IH, WB Human, Mouse Immunogen = KLH conjugated synthetic peptide between 131~161 amino acids from the N-terminal region of human LAMP1 recognizes LAMP1, 44.8 kDa by WB -
Abcam monoclonal
IgG1
Mouse
(liquid, IgG fraction, borate, no preservativeAlso available as Phycoerythrine conj. ab25367)
FC, IF, IH, WB Human, Monkey, Mouse - recognizes LAMP1, ~48 kDa by WB -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IH, IP, WB Human, Mouse, Rat immunogen = peptide corresp. to amino acids 1-145 representing full length Tom20 of human origin recognizes Tom20 -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified, PBS, azide, gelatin)
ELISA, IF, WB Human, Mouse immunogen = apeptide mapping within an internal region of OPA1 (dynamin family) of human origin. for detection of Optic atrophy 1 gene protein -
Chemicon and Upstate are Millipore polyclonal
IgG1
Rabbit
(liquid, raw rabbit serum)
WB Human, Mouse, Rat - recognizes mitofilin, mitochondrion inner membrane protein -
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, FITC conj.)
IF Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
R&D Systems monoclonal
IgG2a
Mouse
(filtered solution, protein-G affinity purified, PBS, trehalose, lyophilized)
IH, WB Human immunogen = purified, E. coli-derived, recombinant human High Temperature Requirement Protein-2 (rhHtrA2; aa 134-458) detects the mitochondria-processed form of endogenous human HtrA2/Omi. -
BD Transduction monoclonal
IgG2a
Mouse
(Aqueous buffered solution, affinity purified, BSA, glycrol, azide)
IF, WB Human, Mouse, Rat immunogen = rat Tim23 aa. 5-126. detects Tim23, 23 kDa by WB -
Sigma Aldrich polyclonal
Rabbit
(buffered aqueous solution, affinity isolated, PBS, azide)
IB, IF Mouse, Rat immunogen = a synthetic peptide corresponding to amino acid residues 404-415 of mouse LAMP2 with N-terminal added cysteine, conjugated to KLH detects LAMP-2 -
R&D Systems polyclonal
IgG
Rabbit
(filtered solution, affinity purified, PBS, trehalose, lyophilized)
IC, WB Human, Mouse immunogen = E. coli­derived recombinant human HTRA2/Omi Ala134­Glu45 detects full length and mitochondria ­processed HTRA2/Omi -
BD Transduction monoclonal
IgG1
Mouse
(aquaeous buffered solution, BSA, glycerol, azide, affinity purified)
IF, WB Dog, Human, Mouse, Rat immunogen = Rat GM130, aa. 869-982 recognizes GM130, 130 kDa -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin.Also available as sc-18822 PE or sc-18822 FITC conjugates for FC, and Alexa Fluor® for FC or IF)
FC, IC, IP, WB Human immunogen = adherentspleen cells of human origin detects LAMP-2 -