Neurofilament 150&200kD (JJ29?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (D21?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain
150 & 200 kD, phosphatase sensitive
-
Neurofilament 150&200kD (JJ28?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (JJ32?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150&200kD (JJ42?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific
-
Neurofilament 150&200kD (JJ47?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya
-
Neurofilament 150&200kD (JJ51?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axon& perikarya
-
Neurofilament 150&200kD (JJ8?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 150kD
NF 150 & 200 kD, COOH terminal tail domain, axonspecific, phosphatase insensitive
-
Neurofilament 150kD (C21?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain Neurofilament
NF 150 kD and GFAP, amino terminal rod, phosphatase insensitive,does not stain astrocytes
-
Neurofilament 200kD (A9?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = chicken brain
NF 200 kD, axon specific, phosphatase sensitive
-
Neurofilament 200kD (FF1?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, COOH terminal tail domain, phosphatase sensitive
-
Neurofilament 200kD (II19?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, cell bodies positive, phosphatase insensitive
-
Neurofilament 200kD (II20?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, cell bodies positive, phosphatase insensitive
-
Neurofilament 200kD (II20?)
Dahl and Bignami Lab, William Patterson Universitycontact Eileen Gardner
monoclonal
-
-
immunogen = bovine brain Neurofilament, 200kD
NF 200 kD, phosphatase sensitive
-
Neurofilament H
RMO24(Virginia Lee, U of Penn, CNDR, Philadelphia, PA)used in paper by Giasson, 2002
monoclonal
-
-
phosphorylated NF heavy subunit
-
Neurofilament 70kD
Fitzgerald
monoclonal
IgG
Mouse
-
-
-
Neurofilament 68 kD (NR4)
Sigma Aldrich
monoclonal
IgG1
Mouse
(ascites, azide)
-
Chicken, Pig, Human, Rat
immunogen = neurofilaments purified from pig spinal cord
68 kD neurofilament polypeptide in cultured cells or tissue preparations, not with other intermediate filaments
-
Neurofilament, Pan-Axonal (SMI-312)
None
monoclonal
IgG1 cocktail
Mouse
(liquid, ascites, concentrate)
-
Mammal (various)
-
visualizes axons in an area-specific maturation patternin human fetal brain; visualizes aberrantly sprouting axons in neuriticplaques derived from cortico-cortical fibers in A.D. and identifies loss of synaptic circuitry
Discontinued
Neurofilament 68 kD
Chemicon and Upstate are Millipore
polyclonal
Rabbit
(antiserum, azide)
-
Bovine, Human, Monkey, Mouse, Rat
immunogen = highly purified monkey neurofilament polypeptide
Stains 68 kD neurofilaments in neurons of the centraland peripheral nervous system. Preferentially stains nerve cell bodies.Low reactivity with NF-M or NF-H
-
Neurofilament 200 kD (N52)
Sigma Aldrich
monoclonal
IgG1
Mouse
(ascites, azide)
-
Rabbit, Pig, Human, Monkey, Hamster, Mouse, Rat
epitope in the tail domain of enzymatically de phosphorylatedpig neurofilament H-subunit
200kD neurofilament protein, phosphor. and non phosphor.
-
Neurofilament H (NF-01)
None
monoclonal
IgG1
Mouse
(affinity purified, PBS, azide)
-
Various species
immunogen = pellet of pig brain cold stable proteins after microtubule depolymerization
reacts with conserved phosphory. epitope of NF-H
discontinued
Neurofilament M (NF-09)
None
monoclonal
IgG2a
Mouse
(affinity purified, PBS, azide)
-
Various species
immunogen = pellet of pig brain cold stable proteins after microtubule depolymerization
reacts with both phosphory. and non-phosphory. forms of NF-M
discontinued
Neurofilament 200 kD
Sigma Aldrich
polyclonal
IgG
Rabbit
(IgG fract, PBS, azide)
-
Various species
immunogen = purified neurofilament 200 from bovine spinalcord
200 kD neurofilament polypeptide
-
Neurofilament H & M, phospho (SMI-31)
None
monoclonal
IgG1
Mouse
(liquid, ascites, concentrate)
-
Mammal (various), Chicken, Frog
phosphorylated epitope in extensively phosphorylated neurofilament H
phosphorylated neurofilament H and, to a lesser extent, neurofilament M is detected in most mammalian species, in thick and thin axons but not cell bodies
Discontinued
Neurofilament 160/200 kD
Autogen Bioclear
monoclonal
IgG1
Mouse
-
Human
phosphory- lated epitope present on 160 & 200 kD Neurofilament polypeptides
-
α-Internexin (2E3)
A B R - Thermo Scientific
monoclonal
IgG1
Mouse
-
Mouse
-
-
-
Neurofilament 160/200 kD (MIG-N18)
Pierce Thermo Scientific
monoclonal
IgG1 κ
Mouse
(liquid, ascites, azide)
-
Human
phosphory- lated epitope on the 160- and 200-kD polypeptidesof purified human neurofilament
Human neurofilament
-
Neurofilament H non-phospho (SMI-32)
None
monoclonal
IgG1
Mouse
(liqiud, ascites, concentrate)
-
Mammal (various)
non phos- phorylated epitope neurofilament H
neuronal cell bodies, dendrites and some thick axonsin the central and peripheral nervous systems, but not thin axons;reflects extent of aberrant phosphorylation of neurofilaments in Alzheimer'sdisease. SMI 32 visualizes two bands (200 and 180 kDa) which merge into a single NFH line on two-dimensional blots
Discontinued
Neurofilament (200 kDa & 68 kDa)
Anaspec
monoclonal
IgG1 ,κ
Mouse
(liquid, supernatant, PBS, BSA, azide)
-
Cat, Rabbit, Human, Mouse, Rat
immunogen = a neurofilament purified from human brain
reacts with neurofilament
-
Neurofilament 165 kD (2H3)
DSHB U. Iowa
monoclonal
IgG1
Mouse
-
Mouse, Rat
immunogen = rat brain membranes
Neurofilaments 165 kD
-
Neurofilament 160kD (BF10)
Novocastra
monoclonal
IgG2a
Mouse
(supernat, lyophilized, azide)
-
Human
immunogen = Triton x 100 insoluble rat brain protein
Neurofilament 160kD, cytoplasmic labelling of neuronalaxons
-
Neurofilament 160kD (NN-18)
Novocastra
monoclonal
IgG1
Mouse
(supernat, lyophilized, azide)
-
Human
immunogen = porcine spinal cord
Neurofilament 160kD, cytoplasmic labelling of neuronalaxons
-
Neurofilament 200kD (N52.1.7)
Novocastra
monoclonal
IgG1
Mouse
(supernat, lyophilized, azide)
-
Human
immunogen = Carboxy-terminal segment of enzymatically dephosphorylated porcine neurofilament H
both phosphorylated and un phosphorylated forms of Neurofilament200kD, cytoplasmic labelling of neuronal axons
-
Neurofilament 200kD (RT97)
Novocastra
monoclonal
IgG1
Mouse
(supernat, lyophilized, azide)
-
Human
immunogen = Triton x 100 insoluble rat brain protein
Neurofilament 200kD, cytoplasmic labelling of neuronalaxons
-
Neurofilament 68kD (DA2)
Novocastra
monoclonal
IgG1
Mouse
(supernat, lyophilized, azide)
-
Amphibian (various), Bovine, Porcine, Mouse, Rat
immunogen = enzymatically dephosphorylated porcine neurofilaments
Human neurofilament 68kD, cytoplasmic labelling of neuronalaxons
-
Neurofilament 68kD (NR4)
Novocastra
monoclonal
IgG1
Mouse
(supernat, lyophilized, azide)
-
Mammal (various)
immunogen = porcine spinal cord
Porcine neurofilament 68kD, cytoplasmic labelling ofneuronal axons
-
Neurofilament NF-L (2F11)
DAKO
monoclonal
IgG1 κ
Mouse
(supernatant, Tris, azide)
-
Cat, Dog, Rabbit, Cow, Horse, Opossum, Swine, Human, Mouse, Rat
immunogen = neurofilament isolated from normal adult human brain
labels neurons, neuronal processes, peripheral nerves, etc; cytoplasmic, 70 kDa unit (NF-L) in WB
-
Neurofilament NF-L (2F11)
DAKO
monoclonal
IgG1 κ
Mouse
(ready to use, buffered, stabilizer, azide)
-
Human
immunogen = neurofilament isolated from normal human adult brain
specifically labels neuronal cells; intended for use inimmunohistochemistry together with Autostainer Link instruments; labels neurons (axons) of the central andperipheral nervous system
-
Neurofilament NF-L (2F11)
DAKO
monoclonal
IgG1 κ
Mouse
(ready to use, buffered, stabilizer, azide)
-
Human
immunogen = neurofilament isolated from normal human adult brain
intended foruse in IH together with Dako Autostainer /Autostainer Plus instruments; labels neurons of the central andperipheral nervous system
-
Neurofilament (RT97)
DSHB U. Iowa
monoclonal
IgG1
Mouse
-
Chicken, Human, Rat
immunogen = semi-purified adult Wistar rat neurofilament
poly phosphorylated epitopes on neurofilaments, and toa lesser extent, on other molecules, e. g. rhodopsin and histones
-
Neurofilament Associated Antigen (3A10)
DSHB U. Iowa
monoclonal
IgG1
Mouse
-
Chicken, Mouse, Rat
immunogen = chick nervous tissue, assorted
Neurofilaments associated
-
Neurofilament 165 kD (B10)
DSHB U. Iowa
monoclonal
IgM
Mouse
-
Mouse, Rat
immunogen = mouse (stage E18) mid- and hindbrain (wholecells)
Neurofilaments 165 kD, in maturing primary sensory andmotor neurons
-
Neurofilament Associated Protein (E/C8)
DSHB U. Iowa
monoclonal
IgM
Mouse
-
Avian (various)
immunogen = quail, embryonic day 7 dorsal root ganglia
neurofilament associated protein, NAPA-73
-
Neurofilaments, primary sensory and motor (E1.9)
DSHB U. Iowa
monoclonal
IgM
Mouse
-
Mouse, Rat
immunogen = mouse (stage E11) CNS (whole cells)
Neurofilaments 165 kD, in growing primary sensory andmotor neurons
-
Neurofilament 160 kD (4H6)
DSHB U. Iowa
monoclonal
IgG
Mouse
-
Avian (various)
immunogen = chick optic nerve, developmental stage E10
Neurofilaments 160 kD
-
Neurofilament (2F11)
Diagnostic Biosystems
monoclonal
IgG1 ,κ
Mouse
(tissue culture supernatant, azide)
-
Human
immunogen = purified neurofilaments from human brain
reacts with the 70 and 200 kDa component of the polypeptide subunit
-
Neurofilament 200kD (RT97)
Diagnostic Biosystems
monoclonal
IgG1
Mouse
(tissue culture supernatant, azide)
-
Rat
immunogen = Triton x 100 insoluble rat brain protein
stains strongly with neurofilaments of 200 kD
-
Neurofilament (2F11)
Diagnostic Biosystems
monoclonal
IgG1 ,κ
Mouse
(pretitered, ready to use)
-
Human
immunogen = purified neurofilaments from human brain
reacts with the 70 and 200 kDa component of the polypeptide subunit
-
Neurofilament H (RMdO 20)
Cell Signaling
monoclonal
IgG1
Mouse
(liquid, purified Ig, azide)
-
Human, Mouse, Rat
immunogen = rat neurofilament
detects endogenous levels of total Neurofilament-H protein, 180-220 kDa
-