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Antibodies

39 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Gap Junctions From labs (1)
antibodies developed by MA Bhat, Reference: Ziskin et al,, 2007 polyclonal
Guinea Pig
- - immunogen = sequences corresp. to the cytoplasmic region of NCP1 NCP1 -
Gap Junctions From manufacturers (38)
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
IF, IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide derived from the mid region of human ZO-1, which differs from dog, mouse, and rat by one conservative amino acid replacement reacts with the ~225 kDa ZO-1 protein; bands of unknown origin may be observed at lower molecular weights. -
Zymed/ Invitrogen monoclonal
IgG1
Mouse
(ascites, PBS, azide, protein-A purifiedAlso available FITC conj; 33-9111)
IF, WB Dog, Human immunogen = human recomb. ZO-1 fusion protein encompassing amino acids 334-634 reacts with the ZO-1α+ and ZO-1α- isoforms; is specific for ZO-1 proteins; cross-reactivity with the related ZO-2 protein has not been observed -
Zymed/ Invitrogen monoclonal
IgG1
Mouse
(ascites, PBS, azide, protein-A purifiedAlso available FITC conj, 33-1511 andHRP conj, 33-1520)
ELISA, IF, WB Dog, Human, Mouse, Rat immunogen = GST fusion protein consisting of the C-terminal (150 a.a.) of human occludin fused to GST reacts specifically with mammalian occludin -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
IF, IH, WB Human immunogen = synthetic peptide derived from the N-terminal region of the human, rat, mouse, dog, and orangutan occludin proteins reacts specifically with occludin -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
IF, IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide derived from the C-terminal region of the human occludin protein reacts with occludin, ~65 kDa -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
IF, WB Dog, Human immunogen = GST-fusion protein containing the C-terminal region of human occludin protein reacts with occludin, ~65 kDa -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified using a GST-Occludin coupled gel)
ELISA, IF, IP, WB Dog, Human, Mouse, Rat immunogen = GST fusion protein consisting of the C-terminal 150 aa of human occludin fused to GST reacts specifically with mammalian occludin -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
ELISA, IH, IP, WB Dog, Human, Guinea Pig, Mouse, Rat immunogen = 69 kD fusion protein corresp. to aa 463-1109 of human ZO-1 cDNA; the sequence lies N-terminal to the 80 aa region (the α-motif) present in the a+ isoform but absent in the a- isoform due to alternative splicing reacts with the ZO-1α+ and ZO-1α- isoforms. Reactivity with dlg has not been characterized -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserua, PBS, BSA, azide, purified)
IH Human immunogen = peptide sequence from the N-terminal region of human ZO-1 staining of ZO-1, in membrane, and may display cytoplasmic staining in tumor cells -
Zymed/ Invitrogen polyclonal
Ig
Rabbit
(antiserum, PBS, azide, affinity purified)
IF, IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide derived from the N-terminal region of human, dog, mouse, and rat ZO-1 reacts with the ~225 kDa ZO-1 protein; bands of unknown origin may be observed at lower molecular weights. -
Abcam polyclonal
IgG
Rabbit
(liquid, PBS, BSA, azide, affinity purified)
IC, IF, WB Human, Mouse, Rat immunogen = synthetic peptide derived from within residues 1350 to the C-terminus of mouse Caspr, conjugated to KLH Caspr/NCP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping near the N-terminus of human Caspr Caspr/NCP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping near the C-terminus of human Caspr Caspr/NCP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping near the N-terminus of human Caspr Caspr/NCP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping within an internal region of human Caspr2 Caspr2 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = epitope corresp. to aa1251-1316 mapping near the C-terminus of human Caspr Caspr/NCP1 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human immunogen = epitope mapping near the N-terminus of human Caspr2 Caspr2 -
Santa Cruz polyclonal
IgG
Goat
(liquid, PBS, azide, gelatin, affinity purified)
ELISA, IF, WB Human, Mouse, Rat immunogen = epitope mapping near the C-terminus of human Caspr2 Caspr2 -
Novus Biologicals polyclonal
Mouse
(mouse antisera, glycerol, no preservative)
ELISA, WB Human immunogen = OCLN (1 a.a. ~ 522 a.a) human protein reacts with occludin -
Novus Biologicals monoclonal
IgG
Rat
(ascites, azide)
IH, IP, WB Dog, Porcine, Mouse, Rat immunogen = DOC-JR-plasma membranes from mouse liver reacts with ZO-1 (membrane associated, non-integralmembrane protein) -
Invitrogen Corporation monoclonal
IgG1
Mouse
(affinity purified, liquid, PBS, azide)
ELISA, IH, WB Human, Mouse, Rat immunogen = Synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26 monoclonal reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5 kDa; anti-Cx26 appears to exhibit minimal cross reaction with Cx30 by western blotting -
Invitrogen Corporation monoclonal
IgG2a
Mouse
(protein-A purified, liquid, PBS, azide)
ELISA, IH, WB Human, Mouse, Rat immunogen = a 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single amino acid and from the human sequence by two (non-consecutive) amino acids monoclonal reacts with the Connexin 26 protein; no cross-reactivity with the closely related Connexin 30 protein has been observed. -
Invitrogen Corporation monoclonal
IgG1
Mouse
(protein-A purified, liquid, PBS, azide)
IF, WB Mouse, Rat immunogen = synthetic peptide derived from rat Connexin 43 monoclonal reacts specifically with both phosphorylated and non-phosphorylated forms of Connexin 43; does not react with the Connexin 43 dimer, but reacts with non phosphorylated forms migrating at ~41 kD and phosphorylated forms migrating between 43 kD and 45 kD -
Invitrogen Corporation polyclonal
Rabbit
(antiserum, affinity purified, PBS, azide)
IH, WB Mouse, Rat immunogen = a 13 amino acid synthetic peptide derived from the C-terminus of the mouse Connexin 26 protein. This mouse sequence differs from the rat sequence by a single amino acid and from the human sequence by two (non-consecutive) amino acids polyclonal detects the Connexin 26 protein; no cross-reactivity with the closely related Connexin 30 protein has been observed -
Invitrogen Corporation polyclonal
Rabbit
(affinity purified, liquid, PBS, azide)
ELISA, IF, IH, WB Mouse, Rat immunogen = peptide corresponding to a portion of the cytoplasmic loop of rat Connexin-26 polyclonal detects predominantly Connexin 26, 26 kDa; a few cross reactive bands were detected in the 45-50 kDa range -
Invitrogen Corporation polyclonal
Rabbit
(affinity purified, liquid, HEPES, saline, BSA, glycerol)
WB Rat immunogen = phosphopeptide corresp. to amino acid residues surrounding the phosphoserine 368 of rat connexin 43 polyclonal specific for the ~43 kDa connexin 43 protein phosphorylated at serine 368 -
Invitrogen Corporation monoclonal
IgG1
Mouse
(affinity purified, liquid, PBS, azide)
ELISA, EM, IF, IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresp. to a cytoplasmic sequence located near the C-terminus of rat Connexin 43 monoclonal CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated, but will recognize Connexin 43 if it is phosphorylated at sites besides Ser368 -
Invitrogen Corporation monoclonal
IgG1
Mouse
(ascites, protein-A purified, liquid, PBS, BSA, azide, Alexa Fluor 488 conj.)
ELISA, EM, IF, IH, WB Dog, Human, Mouse, Rat immunogen = synthetic peptide corresponding to a cytoplasmic sequence located near the C-terminus of rat Connexin 43 monoclonal CX-1B1 is specific for one of the unphosphorylated forms of Connexin 43; CX-1B1 recognizes Connexin 43 only when the serine at residue 368 is unphosphorylated. CX-1B1 will, however, recognize Connexin 43 if it is phosphorylated at sites besides Ser368. -
Santa Cruz polyclonal
Rabbit
(purified, liquid, PBS, azide, gelatin)
ELISA, IF, IH, IP, WB Human, Mouse immunogen = apeptide mapping near the C-terminus of connexin 26 of human origin polyclonal detects Connexin 26, 26 kDa -
Santa Cruz polyclonal
Goat
(affinity purified, liquid, PBS, azide, gelatinAlso available as rabbit rabbit, sc-7261-R)
ELISA, IF, IP, WB Human, Mouse, Rat immunogen = a peptide mapping at the N-terminus of connexin 26 of human origin polyclonal for detection of connexin 26, and toa lesser extent, connexin 30 -
Santa Cruz monoclonal
IgG1
Mouse
(liquid, PBS, azide, gelatin)
IF, IP, WB Dog, Human, Mouse, Rat immunogen = aminoacids 241-254 within the cytoplasmic domain of connexin 43 of rat origin monoclonal CXN-6 is recommended for detection of connexin 43. -
Santa Cruz monoclonal
IgM
Mouse
(ascites, azide)
IF, IH, IP, WB Cow, Human, Mouse, Rat immunogen = aminoacids 362-381 of connexin 43 of human origin monoclonal CXN-6 is recommended for detection of connexin 43. -
Santa Cruz polyclonal
IgG
Goat
(liquid, affinity purified IgG, PBS, azide, gelatin.Also available sc-6560 AC, agarose conj.)
ELISA, IF, IP, WB Dog, Zebra fish, Cow, Human, Mouse, Rat, Xenopus immunogen = a peptide mapping at the C-terminus of connexin 43 of human origin recommended for detection of connexin 43. -
Santa Cruz polyclonal
IgG
Rabbit
(liquid IgG, PBS, azide, gelatin)
ELISA, IF, IP, WB Zebra fish, Human, Mouse, Rat, Xenopus immunogen = aminoacids 233-382 mapping at the C-terminus of connexin 43 of human origin recommended for detection of connexin 43. -
Invitrogen Corporation polyclonal
Rabbit
(affinity purified, liquid, PBS, azide)
ELISA, IH, WB Human, Mouse, Rat immunogen = peptide corresponding to a segment of the 3rd cytoplasmic domain (C-terminal portion) of rat connexin 43 polyclonal detects Connexin 43, 43 kDa; reactivity of this antibody with connexin 43 is independent of phosphorylation status -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(affinity purified, liquid, Tris, BSA, azide)
ELISA, IC, IP, WB Bovine, Mouse, Rat immunogen = a 19 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse Cx43, coupled to KLH immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence shows 100% conserved with rat and bovine, and 84% with chicken and human (16/19 aa). -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(purified, liquid, phosphate buffer, saline, azide)
ELISA, IF, IH, IP, WB Human, Mouse, Rat immunogen = a 23 amino acid C-terminal peptide sequence within the cytoplasmic domain of mouse Cx43 immunogenic peptide sequence is specific for Cx43 and no significant homology is seen with other connexins. The mouse Cx43 peptide sequence is 100% homologous with rat and human Cx43 -
Chemicon and Upstate are Millipore polyclonal
Rabbit
(affinity purified, liquid, Tris, BSA, azide)
ELISA, IH, WB Mouse immunogen = a 19 amino acidpeptide sequence located near the C-terminal, second cytoplasmic domain of mouse Cx26; conjugated to KLH as a carrier protein specific for Connexin-26 -