Tools

Back to the Top

Antibodies

29 Results

Name Source Clonality &
Isotype
Host &
Formulation
Method Reactivity Immunogen/
Epitope
Specificity Availability
Histones & HDACs From manufacturers (29)
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, antiserum, azide, glycerol)
WB Various vertebrate immunogen = synthetic peptide (C-SATVGPK APSGGKKA) corresp. to aa 121-135 of human histone H2A.X recognizes histone H2A.X -
Upstate and Chemicon are Millipore monoclonal
IgG1
Mouse
(liquid, protein-G purified, phosphate buffer, azide, glycerol; also available biotin conj., 16-193, or FITC conj., 16-202A)
IC, IF, WB Various vertebrate immunogen = peptide (C-KATQA [pS]QEY) corresp. to amino acids 134-142 of human histone H2A.X recognizes histone H2A.X phospho. at Ser139 -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, protein-A purified, Tris, NaCl, azide, glycerol)
IP, WB Various vertebrate immunogen = synthetic peptide (C-KATQA [pS]QEY) corresp. to aa 134-142 of human histone H2A.X recognizes histone H2A.X phospho. at Ser139 -
Upstate and Chemicon are Millipore kit
Unknown Host
(liquid, Protein-A Agarose/Salmon Sperm DNA, azide, buffers, EDTA)
ChIP Non-specific uses anti-acetyl Histone H4 polyclonal antibody (06-866) immuno precipitates acetylated histone H4. Works with all eukaryotic cell types. Quantitative PCR detection is recommended. -
Upstate and Chemicon are Millipore kit
Unknown Host
(liquid, Protein-A Agarose/Salmon Sperm DNA, buffers)
ChIP, IP Non-specific uses anti-acetyl Histone H4 polyclonal antibody (06-866) immuno precipitates transcriptionally active chromatin using anti-acetyl-histone H4. -
Chemicon and Upstate are Millipore kit
IgG
Rabbit
(in vitro toxicology assay, needs HCS imaging system equipped with beam-splitters and filters)
IF Various animal immunogen = Phospho-histone H3 for identifying and quantifying phospho-histone H3(Ser10) expression in cellular imaging applications -
Novus Biologicals polyclonal
Rabbit
(antisera, PBS, azide)
WB Human, Mouse immunogen = synthetic peptide corresponding to a sequence within amino acids 100-200 of human Histone H3 specific for Histone H3; ~17 kDa band in WB -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, purified, Tris-glycine, saline, glycerol, azide)
ChIP, WB Chicken, Human, Xenopus, S. cerevisiae immunogen = KLH-conjugated, synthetic peptide corresponding to amino acids 118-126 (CG-AVTKYTSSK) of human Histone H2B Recognizes and is specific for Histone H2B, MW ~15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, polyclonal IgG, buffer, azide, glycerol)
ELISA, IC, IH, WB Human immunogen = KLH-conjugated, synthetic peptide corresp. to amino acids 6-16 of human histone H2B (C-SAPAPKKG [pS]KK) detects phospho-Histone H2B , MW ~15 kDa -
Upstate and Chemicon are Millipore monoclonal
IgG
Rabbit
(liquid, protein-A purified, Tris-glycine, saline, azide, glycerolAvailable as FITC conj, 16-251)
FC Human immunogen = peptide containing the sequence KGpSKK in which pS corresponds to phospho-serine 14 of human histone H2B detects histone H2B phosphorylated on Serine 14, MW ~15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, purified, glycerol, azide)
WB Various vertebrate, Human immunogen = synthetic peptide containing an acetyl-lysine at residue 5 of human Histone H2B specific for acetyl-histone H2B (Lys5), 14-16 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, antiserum, glycerol, azide)
WB Human immunogen = peptide APAPK AcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F specific for acetyl-histone H2B (Lys12), 15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, antiserum, glycerol, azide)
WB Human immunogen = peptide containing the seqeunce GSAcKKAI in which AcK corresponds to acetyl-lysine at residue 15 of human Histone H2B specific for acetyl-histone H2B (Lys15), 15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, antiserum, glycerol, azide)
WB Human immunogen = peptide containing AITAcKAQ in which AcK corresponds to acetyl-lysine at residue 20 of human Histone H2B specific for acetyl-histone H2B (Lys20), 15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, Tris-glycine, saline, glycerol, azide)
WB Human immunogen = synthetic peptide in which there is an acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B specific for acetylated histone H2B, 15 kDa -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, Tris-glycine, glycerol, azide)
DB, WB Human immunogen = peptide containing the sequence VTacKYT in which acK corresponds to acetylated lysine 120 of human histone H2B specific for Histone H2B acetylated on lysine 120, ~15 kDa -
Cell Signaling polyclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerol)
FC, IC, IF, IH, WB Human, Mouse, Rat immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingSer139 of human H2A.X detects endogenous levels of histone H2A.X phospho. at Ser139, 15 kDa -
Cell Signaling monoclonal
IgG
Rabbit
(liquid, HEPES, saline, BSA, glycerolAlso available Alexa Fluor® 488, Cat# 9719Alexa Fluor® 647, Cat# 9720)
FC, IC, IF, IH, WB Human, Mouse immunogen = synthetic phospho-peptide(KLH-coupled) corresp. to residues surroundingSer139 of human H2A.X detects histone H2A.X phospho. at Ser139 -
Cell Signaling polyclonal
IgG
Rabbit
(liquid, affinity purified, HEPES, saline, BSA, glycerol)
IC, IF, WB Human, Monkey immunogen = synthetic peptide (KLH-coupled) corresp. to residues near the carboxy-terminus of human histone H2A.X recognizes endogenous levels of histone H2A.X independent of phosphorylation and ubiquitination -
AnaSpec monoclonal
IgG2a
Mouse
(liquid, ascites, protein-A purified, PBS, BSA, azide)
IF, IH Human immunogen = a nuclei of myeloid leukemia biopsy cells detects Histone H1 -
Upstate and Chemicon are Millipore monoclonal
IgG
Rabbit
(liquid, protein A purified rabbit IgG in Tris-Glycine, saline, glycerol, azide)
ChIP, WB Mammal (various), Human, Mouse immunogen = a BSA-conjugated, synthetic peptide containing the sequence …RT[me3K]QT… in which me3K corresponds to trimethyl-lysine 4 of human histone H3 specific for Trimethyl-Histone H3 (Lys4) -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, rabbit IgG in Tris-Glycine, saline, glycerol, azide)
WB Mouse, Rat immunogen = a KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes specific for MeCP2 -
Upstate and Chemicon are Millipore monoclonal
IgG
Rabbit
(liquid, supernatant, azide)
ChIP, WB Chicken, Human, Mouse, Rat, S. cerevisiae immunogen = KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3 recognizes Histone H3 near the C-terminus -
Upstate and Chemicon are Millipore polyclonal
Rabbit
(liquid, Protein-A purified, Tris-glycine, saline, azide, glycerol)
ChIP, IC, WB Human, Mouse, Rat immunogen = KLH-conjugated peptide corresponding to amino acids 1-20 of human histone H3 (ARTKQTARKST GGKAPRKQLC) detects histone H3, Mr 17 kDa. Does not effectively recognize acetylated/phosphor. histone H3. -
Upstate and Chemicon are Millipore polyclonal
Rabbit
(liquid, whole antiserum, unpurified, azide)
ChIP, WB Eukaryote, Human, Tetrahymena immunogen = KLH-conjugated peptide corresp. to a.a. 2-19 of Tetrahymena histone H4 (AGGAcKGGAcKGM GAcKVGAAcKRHS-C), acetylated on lysines 5, 8, 12 and 16 specific for acetylated histone H4, 10 kDa; weakly cross-reacts with acetylated histone H2B from HeLa cells -
Upstate and Chemicon are Millipore polyclonal
Rabbit
(liquid, purified, Tris-glycine, saline, azide)
ChIP, IC, IP, WB Human, Mouse, Tetrahymena immunogen = KLH-conjugated peptide corresp. to a.a. 1-20 of Tetrahymena histone H3(ARTKQTAR[K*] STGG[K*] APRKQLC), where K* is acetylated specific for acetylated histone H3 -
Upstate and Chemicon are Millipore polyclonal
IgG
Rabbit
(liquid, rabbit serum, azide)
ChIP, WB Mammal (various), Human, Mouse immunogen = a KLH-conjugated, branched synthetic peptide containing the sequence ..Rme2KSTG.., in which me2K corresponds to dimethyl-lysine at residue 9 of human histone H3 specific for Dimethyl-Histone H3 (Lys9) -
Abcam polyclonal
IgG
Rabbit
(liquid, whole antiserum, azide)
ChIP, ELISA, IF, IH, IP, WB Human, Mouse, Rat, S. cerevisiae, S. pombe, Drosophila immunogen = Synthetic peptide: GKGLGKAcGGAKC. specific for Histone H4 acetylated at lysine 12 -
Santa Cruz polyclonal
IgG
Rabbit
(liquid, PBS, azide, gelatin)
ELISA, IF, IP, WB Human immunogen = epitope corresp. to aa 916-1215 mapping at the C-terminus of HDAC6 of human origin detects HDAC6, 160 kDa -