Antibody

Myosin light chain, phos ser19 (MF-20)

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Clonality: monoclonal
Host: Mouse
Isotype: IgG1
Methods: IF
Source: Cell Signaling
Cat# 3675 (Mab) and Cat# 3671 (poly, rabbit).
Reference:
Rex CS, 2010
Formulation: mono: liquid, HEPES, BSA, glycerol, azide;<br />poly: liquid, protein-A affinity purified, HEPES, saline, BSA, glycerol, azide;
Reactivity:
Reacts in: Bovine, Pig, Human, Mouse, Rat
plus chicken, D. melanogaster, Xenopus and zebrafish for the rabbit antibody

Immunogen/Epitope

Immunogen = a synthetic phosphopeptide corresponding to residues surrounding Ser19 of human myosin light chain 2

Specificity

detect endogenous levels of myosin light chain 2 (smooth muscle) only when phosphorylated at serine 19. These antibodies do not cross-react with the cardiac isoform of myosin light chain 2

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References

Paper Citations

  1. . Myosin IIb regulates actin dynamics during synaptic plasticity and memory formation. Neuron. 2010 Aug 26;67(4):603-17. PubMed.

Further Reading

No Available Further Reading