Antibody

FLAG (M2)

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Clonality: monoclonal
Host: Mouse
Isotype: IgG1
Source: Sigma Aldrich
Cat# A9469
References:
Wakabayashi, 2009
Serneels, 2009
Tsuda, 2008
Osawa, 2008
Sato, 2007
(all, unspecified formulation)
Formulation: purified Ig, buffered aqueous glycerol solution, TBS, glycerol, stabilizer, preservative, AP conj.<br />also available as HRP conj., <a href="http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A8592" target="_new">A8592</a> or biotin labeled, <a href="http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/datasheet2/f9291dat.Par.0001.File.tmp/f9291dat.pdf" target="_new">F9291</a>
Reactivity:
Reacts in: Various species, Mouse, Rat

Specificity

Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent; useful in detection of FLAG fusion proteins expressed in murine host

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References

Paper Citations

  1. Wakabayashi T, Craessaerts K, Bammens L, Bentahir M, Borgions F, Herdewijn P, Staes A, Timmerman E, Vandekerckhove J, Rubinstein E, Boucheix C, Gevaert K, De Strooper B. Analysis of the gamma-secretase interactome and validation of its association with tetraspanin-enriched microdomains. Nat Cell Biol. 2009 Nov;11(11):1340-6. PubMed.
  2. Serneels L, Van Biervliet J, Craessaerts K, Dejaegere T, Horré K, Van Houtvin T, Esselmann H, Paul S, Schäfer MK, Berezovska O, Hyman BT, Sprangers B, Sciot R, Moons L, Jucker M, Yang Z, May PC, Karran E, Wiltfang J, D'Hooge R, De Strooper B. gamma-Secretase heterogeneity in the Aph1 subunit: relevance for Alzheimer's disease. Science. 2009 May 1;324(5927):639-42. Epub 2009 Mar 19 PubMed.
  3. Tsuda H, Han SM, Yang Y, Tong C, Lin YQ, Mohan K, Haueter C, Zoghbi A, Harati Y, Kwan J, Miller MA, Bellen HJ. The amyotrophic lateral sclerosis 8 protein VAPB is cleaved, secreted, and acts as a ligand for Eph receptors. Cell. 2008 Jun 13;133(6):963-77. PubMed.
  4. Osawa S, Funamoto S, Nobuhara M, Wada-Kakuda S, Shimojo M, Yagishita S, Ihara Y. Phosphoinositides suppress gamma-secretase in both the detergent-soluble and -insoluble states. J Biol Chem. 2008 Jul 11;283(28):19283-92. PubMed.
  5. Sato T, Diehl TS, Narayanan S, Funamoto S, Ihara Y, De Strooper B, Steiner H, Haass C, Wolfe MS. Active gamma-secretase complexes contain only one of each component. J Biol Chem. 2007 Nov 23;282(47):33985-93. PubMed.

External Citations

  1. A8592
  2. F9291

Further Reading

No Available Further Reading