Amyloid-β protofibrils

Clonality: monoclonal
Host: Unknown Host
Isotype: IgG2a
Methods: ELISA, IB, WB
Source: mAb158
(Hillevi Englund, Department of Public Health/Molecular Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden)
Lord, 2009
Englund, 2007.
Recently, Anna Lord reported that the mAb158 sandwich ELISA detects synthetic protofibrils, as well as protofibrils in biological samples from a variety of mouse models
Formulation: supernatant, protein-G purified
Reacts in: Human


immunogen = 30 μg Aβ1–42 Arc protofibrils


beta amyloid protofibrils; does not bind to other amyloids or to amyloid precursor protein (APP). The conformation selectivity of mAb158 is exclusive for amyloid-β (Aβ) as it did not display any binding to other amyloid fibrils; mAb158 did not immuno precipitate α-APPs from cell media or full-length APP from mouse brain homogenate; binds to Aβ fibrils in addition to oligomeric Aβ; has some cross-reactivity with monomeric Aβ

Comments / Questions

No Available Comments

Make a comment or submit a question

To make a comment you must login or register.


News Citations

  1. San Diego: Oligomers Live Up to Bad Reputation, Part 2

Paper Citations

  1. . An amyloid-beta protofibril-selective antibody prevents amyloid formation in a mouse model of Alzheimer's disease. Neurobiol Dis. 2009 Dec;36(3):425-34. PubMed.
  2. . Sensitive ELISA detection of amyloid-beta protofibrils in biological samples. J Neurochem. 2007 Oct;103(1):334-45. PubMed.

Further Reading

No Available Further Reading