Host: Unknown Host
Methods: ELISA, IB, WB
(Hillevi Englund, Department of Public Health/Molecular Geriatrics, Rudbeck Laboratory, Uppsala University, Uppsala, Sweden)
Recently, Anna Lord reported that the mAb158 sandwich ELISA detects synthetic protofibrils, as well as protofibrils in biological samples from a variety of mouse models
Formulation: supernatant, protein-G purified
Reacts in: Human
immunogen = 30 μg Aβ1–42 Arc protofibrils
beta amyloid protofibrils; does not bind to other amyloids or to amyloid precursor protein (APP). The conformation selectivity of mAb158 is exclusive for amyloid-β (Aβ) as it did not display any binding to other amyloid fibrils; mAb158 did not immuno precipitate α-APPs from cell media or full-length APP from mouse brain homogenate; binds to Aβ fibrils in addition to oligomeric Aβ; has some cross-reactivity with monomeric Aβ
- Lord A, Gumucio A, Englund H, Sehlin D, Sundquist VS, Söderberg L, Möller C, Gellerfors P, Lannfelt L, Pettersson FE, Nilsson LN. An amyloid-beta protofibril-selective antibody prevents amyloid formation in a mouse model of Alzheimer's disease. Neurobiol Dis. 2009 Dec;36(3):425-34. PubMed.
- Englund H, Sehlin D, Johansson AS, Nilsson LN, Gellerfors P, Paulie S, Lannfelt L, Pettersson FE. Sensitive ELISA detection of amyloid-beta protofibrils in biological samples. J Neurochem. 2007 Oct;103(1):334-45. PubMed.
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